Plant growth-promoting complex microbial inoculant produced based on ecological relationship among strains and application thereof

A compound bacterial agent and plant technology, which is applied in the field of agricultural microorganisms, can solve the problems of unstable field application effect and difficult to guarantee the biological function of microbial fertilizers.

Active Publication Date: 2021-08-31
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, the main bottleneck restricting the development of the bio-fertilizer industry is the instability of field application effects. After the functional microorganisms in the fertilizer are a

Method used

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  • Plant growth-promoting complex microbial inoculant produced based on ecological relationship among strains and application thereof
  • Plant growth-promoting complex microbial inoculant produced based on ecological relationship among strains and application thereof
  • Plant growth-promoting complex microbial inoculant produced based on ecological relationship among strains and application thereof

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[0031] 1. Strain screening and isolation and identification

[0032] (1) Primary screening of strains and identification of 16S rDNA

[0033] The strain SQR9-gfp was inserted into the cucumber pot, and the inoculation amount was 10 6 CFU / g, take the cucumber root system after two weeks, wash the root surface soil with deionized pure water, and place it on the semi-solid Swarming medium. Under the microscope, SQR9-gfp emits green fluorescence ( figure 1 ), pick the surrounding fusion strains, streak them on the plate, and perform 16S rDNA sequencing identification to obtain more than 100 strains of bacteria, which are stored in a glycerol tube in a -80°C refrigerator.

[0034] (2) Rescreening and phenotypic identification of strains

[0035] Based on the 16S rDNA sequence, a phylogenetic tree was established, and the strains on the same branch of the phylogenetic tree were subjected to paired strain confrontation experiments using Swarming semi-solid medium ( figure 2 ), ...

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Abstract

The invention provides a plant growth-promoting complex microbial inoculant produced based on ecological relationship among strains and application thereof. The plant growth-promoting complex microbial inoculant is prepared by mixing bacterial liquids of bacillus amyloliquefaciens SQR9, bacillus amyloliquefaciens RF-CU-1, lysine bacillus RF-CU-2 and brevibacillus pulcherrimus RF-CU-3. The bacillus synthetic flora can cooperate with each other and remarkably promote growth of cucumber plants, and under the sterile water culture condition in a laboratory, the growth-promoting effect is remarkably improved along with improvement of diversity of inoculated flora.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a design method for producing a plant growth-promoting compound microbial agent based on the ecological relationship among bacterial strains and its application. Background technique [0002] Plant rhizosphere microorganisms are considered to be the second genome of plants, which play a key role in the host's nutrient absorption and assisting plants in coping with various biotic and abiotic stresses. The application of plant rhizosphere growth-promoting bacteria to improve crop yield and quality is a modern One of the important trends in the development of green agriculture. At present, the main bottleneck restricting the development of the bio-fertilizer industry is the instability of field application effects. After the functional microorganisms in the fertilizer are applied to the soil, they face the rejection and competition of indigenous microorganisms, and it is dif...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N63/20A01P21/00C12R1/07C12R1/01
CPCC12N1/20A01N63/22A01N63/20
Inventor 沈其荣徐志辉刘妍张瑞福邵佳慧解继驭
Owner NANJING AGRICULTURAL UNIVERSITY
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