Common rapid detection method for various pesticide residues in soybeans

Abstract

The invention discloses a common rapid detection method for various pesticide residues in soybeans. The common rapid detection method is characterized by particularly comprising the following steps: homogenizing and extracting a soybean sample by acetonitrile; carrying out salting-out and centrifuging to obtain a supernatant; purifying by using a solid phase extraction column to obtain a sampling solution; preparing a mixed standard solution and preparing a base material adding standard curve; simultaneously detecting 306 types of pesticide residues in the soybeans by a gas chromatography-tandem mass spectrometer; finally, calculating the concentration. The common rapid detection method has the advantages that the effect of the base material and background interferences are reduced and the anti-interference capability of the method is improved greatly; the analysis sensitivity is improved; the detection linear range is 0-0.2microgram/milliliter; the detection is limited to be 0.01mg/kg; the recycling rate is 60%-120%; the relative standard deviation RSD is less than or equal to 20%; the recycling rate is ideal and the precision degree is good; the common rapid detection method is simple and rapid, is low in background interferences, good in selectivity and high in sensitivity.

Description

technical field [0001] The invention relates to a method for detecting pesticide residues, in particular to a general rapid detection method for various pesticide residues in soybeans. Background technique [0002] Pesticides are widely used in agricultural production to increase yields, but the hazards of pesticide residues are also becoming more and more serious. Developed countries have successively established minimum concentration limits for pesticides in vegetables, such as the European guideline 91 / 414 / EEC, the U.S. Food Quality Protection Act (FQPA)] and Japan’s positive list system with the world’s most stringent food limit standards. Since the early 1990s, my country began to study pesticide multi-residue analysis methods, and successively introduced a series of national standard analysis methods. Pesticide multi-residue detection technology has developed rapidly in my country. [0003] At present, for the analysis of multi-component pesticide residues, techniques...

AI Technical Summary

Problems solved by technology

Relatively poor qualitative accuracy due to GC or LC being limited by the detector

With the popularity of mass spectrometry detectors, GC-MS has been widely used. The existing national standards for the determination of multiple pesticide residues in soybeans, such as GB / T19649-2006 Determination of 475 pesticides and related chemical residues in grains, have published Gas chromatography-mass spectrometry is used to detect pesticide residues in fruits and vegetables, but due to the lack of sensitivity of single-stage mass spectrometry itself and poor anti-interference ability, the detection limit (LOQ) is generally between 50-100ug / kg , it is difficult to meet the increasingly stringent pesticide residue testing requirements of the European Union and Japan, especially the uniform standard requirements (10mg / kg) in the positive list system of Japan

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