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Method for simultaneously detecting multiple fungaltoxins in grains

A technology for mycotoxins and grains, which is applied in the field of detection of various mycotoxins in grains. It can solve the problems of complex matrix, few types of immunoaffinity antibodies, and high cost, and achieve improved detection efficiency, high recovery rate of standard addition, and accurate toxin content. Effect

Inactive Publication Date: 2017-05-31
PLANT PROTECTION & QUALITY & SAFETY OF AGRI PRODS INST ANHUI ACAD OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

Immunoaffinity chromatography is a separation system in which one of the antigens and antibodies is used as the ligand for the affinity adsorption of the other. Using this technology to pretreat the sample has strong specificity and a high degree of sample purification. However, the currently developed immunoaffinity chromatography There are few types of affinity antibodies, except for a few compounds (aflatoxins), which cannot purify multi-component compounds at the same time, and the cost is high, and the sample pretreatment operation is complicated. At present, the toxins with immunoaffinity antibodies include aflatoxins , ochratoxin A, zearalenone, T-2 toxin, HT-2 toxin and a few toxins
Solid phase extraction technology is based on the theory of liquid-solid phase chromatography. It uses selective adsorption and selective elution to enrich, separate and purify samples. It is a physical extraction process including liquid phase and solid phase; it can also be Think of it as a simple chromatographic process. This technology is not as selective in adsorption and purification as immunoaffinity technology. It can be used for the simultaneous purification of one or several structurally similar toxins, but it cannot be used for multiple toxins in complex matrices. Simultaneous adsorption and purification, commonly used in the pretreatment of detection of fumonisin, aflatoxin B1 and ochratoxin A in food
The matrix of grains is complex, especially when the purification of the sample matrix is ​​not complete, the target compounds cannot be effectively separated on the chromatographic column, and the target compounds cannot be accurately quantified, and all 10 kinds of mycotoxins cannot be detected by existing methods, so there is no There is a method capable of simultaneously detecting 10 mycotoxins in grains

Method used

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  • Method for simultaneously detecting multiple fungaltoxins in grains
  • Method for simultaneously detecting multiple fungaltoxins in grains
  • Method for simultaneously detecting multiple fungaltoxins in grains

Examples

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Embodiment 1

[0040] (1) Mix no less than 1000g of wheat or rice (hulled) or corn kernels evenly, take samples by quartering, put them into a food processor, grind them, pass through a 20-mesh sieve, make a sample, and put it into a sample bottle Seal it, mark it, and store it at -20°C until it is tested.

[0041] (2) Weigh 21.0 g of the sample, add 50 mL of acetonitrile water mixed solvent containing 1% (volume) acetic acid (acetonitrile: water = 84:16, V: V), shake for 30 min, ultrasonically extract for 10 min, add 5 g of sodium chloride, Centrifuge at 5000r / min for 3min. Take 2 mL of the supernatant, that is, the acetonitrile extract, and wait for purification.

[0042] (3) Take 2 mL of the above-mentioned acetonitrile extract in a 5 mL glass test tube, add 200 mg of anhydrous magnesium sulfate adsorbent, vortex and mix for 1 min, let it stand, pass through a 0.22 μm filter membrane, and obtain a sample analysis solution for liquid chromatography-tandem mass spectrometer Determination....

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Abstract

The invention discloses a method for simultaneously detecting multiple fungaltoxins in grains. The method comprises the steps of extracting a grain sample to be detected through an acetonitrile-water solution containing acid, salting out, purifying, centrifuging, and membrane filtering to obtain sample analytic liquid; utilizing an ultra-high performance liquid chromatography-tandem mass spectrometry for simultaneously detecting the multiple fungaltoxins. According to the method provided by the invention, the fungaltoxins are subjected to qualitation and quantitation by adopting a multi-reaction monitoring mode (MRM). After the grains are subjected to pretreatment, the ultra-high performance liquid chromatography-tandem mass spectrometry is utilized for simultaneously detecting extracted 10 fungaltoxins. The sample is extracted through the acetonitrile-water solution with a certain concentration and containing the acid, a sample extracting solution is salted out and purified, and the multi-reaction monitoring mode of the high performance liquid chromatography-tandem mass spectrometry is utilized for detecting, so that the defection efficiency is improved, the detection cost is rediced, the sensitivity is high, the repeatability is good, and the adding standard recovery is high.

Description

technical field [0001] The invention relates to a method for detecting multiple mycotoxins in grains, in particular to a method for simultaneously detecting 10 kinds of mycotoxins in grains by using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS), which belongs to food safety detection field. Background technique [0002] Mycotoxins are toxic metabolites produced by fungi under suitable environmental conditions, which have been a huge potential threat to humans, animals and plants since ancient times. In 1960, more than 100,000 turkeys were poisoned and died in the United Kingdom. Studies have found that moldy peanut cake powder imported from Brazil was included in the feed. Feeding rats with this peanut cake powder can induce liver cancer and substances that induce liver cancer It was confirmed to be a metabolite of Aspergillus flavus, named as aflatoxins (Aflatoxins). Moldy corn poisoning of horses and cattle, sweet potato black spot p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88G01N2030/884
Inventor 段劲生孙明娜高同春董旭王梅肖青青褚玥
Owner PLANT PROTECTION & QUALITY & SAFETY OF AGRI PRODS INST ANHUI ACAD OF AGRI SCI
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