Technique for preparing sponge produced from collagen

A collagen sponge and preparation process technology, applied in bandages, absorbent pads, medical science and other directions, can solve the problems of poor wound surface adhesion, poor product appearance, low collagen protein content, etc., to increase flexibility and adhesion properties, improve clinical application effect, and improve the effect of hemostasis

Active Publication Date: 2007-02-21
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The collagen produced by this process has low protein content and high salt content, and due to the large amount of collagen precipitated after dialysis, the freeze-dried product has poor appearance, hard texture and poor adhesion to the wound surface, and is easy to fall off from the wound surface affect the hemostatic effect

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0035] The preparation process of collagen sponge is carried out at a temperature of 0-15°C from extraction to dialysis, and the process is as follows:

[0036] Step 1. Preparation of bovine Achilles tendon fragments

[0037] Put the washed beef Achilles tendon slices into the tissue masher, add an appropriate amount of cold distilled water, turn on the machine to mash the beef Achilles tendon into pieces, pick out the fascia, wash with cold distilled water, and soak in normal saline for 1-2 hours , take it out, place it in silk and squeeze dry;

[0038] Step 2. Collagen Extraction

[0039] First, place 100 g of dried bovine Achilles tendon fragments in a glass jar, add 500 ml of 1% acetic acid, and allow it to swell. Then add 500ml of 1% acetic acid containing 10g of sodium chloride, and stir with a mixer for more than 30min. Then add in the 1% acetic acid 1000ml that contains 5 grams of pepsin, make it stir evenly;

[0040] Then after the digestion starts, add acetic aci...

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PUM

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Abstract

A process for preparing the collagen sponge includes such steps as extracting collagen in acetic acid by digestion of pepsinum, filtering, taking supernatant, neutralizing by sodium hydroxide, salting out in sodium chloride solution, centrifugal separation of deposit, dissolving it in acetic acid, dialyzing by acetic acid and then by the solution of bisodium hydrogen phosphate, and freeze drying.

Description

technical field [0001] The invention relates to a preparation process of a collagen sponge, in particular to a purified freeze-dried collagen sponge, which can be used for hemostasis and repair of various burns, wounds and surgical wounds, as well as filling of ulcers and residual cavities. Background technique [0002] Collagen is a large family composed of a variety of glycoprotein molecules. It is the main protein component of connective tissue, accounting for about 25% of the total protein in the body. Collagen is a structural protein of the extracellular matrix in which collagen molecules aggregate into supramolecular structures. Collagen is a family of proteins. More than 16 different types of collagen have been discovered. Type I, type II, type III and type IV collagen are discovered through primary structure analysis at the protein level, and others are Discovered by similarity analysis of collagen domains at the nucleic acid level. Collagen, like other proteins, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32
Inventor 顾其胜蒋丽霞
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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