Method for extracting native natural collagen from animal skin or/and tendon

A collagen and animal skin technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of long extraction time and low yield, save extraction time, increase yield, and save energy Effect

Active Publication Date: 2008-07-30
无锡贝迪生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a new method for extracting undenatured natural collagen from animal skin or/and tendon, to overcome the long extraction tim

Method used

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  • Method for extracting native natural collagen from animal skin or/and tendon

Examples

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Comparison scheme
Effect test

Embodiment 1

[0028] (1) Raw material pretreatment: clean and fresh healthy pigskin is used as raw material. Remove the grease and impurities on the surface of the pigskin as much as possible, and then cut the raw materials into small pieces. After soaking in acetone for about 24hrs, take it out and dry it naturally, then crush the raw materials into crumbs with a pulverizer.

[0029] (2) Enzymolysis: Weigh 10 parts by weight of raw material scraps, add them to a reactor equipped with a stirrer and a thermometer, add 500 parts by weight of 0.5mol / L acetic acid solution, add 0.25 parts by weight of pepsin after soaking for about 1 hour, and then react The device is placed in an ultrasonic water bath with a power of 120W / 5g, and the stirrer is operated at about 20°C for about 24 hours for ultrasonic enzymolysis.

[0030] (3) Centrifugation: Dilute the enzymolysis solution 3 times with 0.5mol / L acetic acid solution and keep stirring. Centrifuge to remove the incomplete part of the enzymolysi...

Embodiment 2

[0035] (1) Raw material pretreatment: clean and fresh bovine Achilles tendon is used as raw material. Remove as much grease and impurities as possible from the surface of the Achilles tendon, then cut the ingredients into small pieces. After soaking in acetone for about 12hrs, take it out and dry it naturally, and then use a pulverizer to crush the raw materials into crumbs.

[0036] (2) Enzymolysis: Weigh 10 parts by weight of raw material scraps, add them to a reactor equipped with a stirrer and a thermometer, add 800 parts by weight of 0.5mol / L acetic acid solution, soak for about 1 hour, add 0.2 parts by weight of pepsin, and then react The device is placed in an ultrasonic water bath with a power of 100W / 5g, and the stirrer is operated at about 20°C for about 12 hours for ultrasonic enzymolysis.

[0037] (3) Centrifugation: Dilute the enzymolysis solution 5 times with 1mol / L acetic acid solution and keep stirring. Centrifuge to remove the incomplete part of the enzymoly...

Embodiment 3

[0042] (1) Raw material pretreatment: use clean and fresh sheepskin as raw material. Try to remove the grease and impurities on the surface of the raw material, and then cut the raw material into small pieces. After soaking in acetone for about 24hrs, take it out and dry it naturally, then crush the raw materials into crumbs with a pulverizer.

[0043] (2) Enzymolysis: Weigh 10 parts by weight of raw material scraps, add them to a reactor equipped with a stirrer and a thermometer, add 250 parts by weight of 1.0mol / L acetic acid solution, soak for about 1 hour, add 0.3 parts by weight of pepsin, and then react The device is placed in an ultrasonic water bath with a power of 120W / 5g, and the stirrer is operated at about 25°C for about 12 hours for ultrasonic enzymolysis.

[0044] (3) Centrifugation: Dilute the enzymolysis solution 3 times with 1.0mol / L acetic acid solution and keep stirring. Centrifuge to remove the incomplete part of the enzymolysis solution to obtain a crude...

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Abstract

The invention discloses a method of extracting non-denaturing natural collagen from the animal skin or / and the tendon, and the method comprises the following steps the raw material chips of the animal skin or / and the tendon in which the lipa and the impurity are removed, and which is soaked and per-processed through acetone is added into a reactor and soaked with acetate solution, stomach cnzyme is added after full soaking, the enzymolysis is performed under the irradiation of the ultrasonic wave, after enzymolysis, enzymolysis liquid is diluted with the acetate solution, the part that is not completely enzyme-decomposed is separated and removed in the enzymolysis liquid, obtained collagen aqueous solution is salted out with 2 mol/L NaCl or (NH4)2SO4 solution, collagen salted object obtained by separating and salting out suspended material is stirred and dialyzed with distilled water, and the product after being dialyzed is frozen out through the freezing dryer, namely, the collogen product is obtained. By adopting the method of the invention to prepare the collogen, the extraction time of the collogen can be greatly saved, the collogen production rate is improved, and the obtained collogen can completely retain the three unique spiral structures, which can be applied to the biological medicine material.

Description

technical field [0001] The invention relates to collagen preparation technology, in particular to a method for extracting undenatured natural collagen from skin or / and tendons of healthy animals. Background technique [0002] Collagen is the most abundant protein in mammals, accounting for 25%-30% of the total protein in the body, equivalent to 6% of body weight. It is the main component of tendon, cartilage, skin and blood vessel proteins, and has a wide range of sources. And because of its unique triple helical structure, as well as good biocompatibility and biodegradability, it is widely used in medical and health fields such as burns, tissue repair, wound hemostasis, corneal diseases, orthopedics, and drug controlled release systems. . [0003] At present, the extraction and preparation methods of collagen mainly include neutral salt method, acid method, alkali method and enzymatic method. As CN 1903918A and CN 1155549A have introduced respectively the method for raw m...

Claims

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Application Information

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IPC IPC(8): C07K1/14C12P21/06
Inventor 穆畅道李德富林炜
Owner 无锡贝迪生物工程股份有限公司
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