Method for extracting and salting organic acids out of fermentation liquor

A fermented liquid and organic acid technology, applied in the field of bioengineering, can solve the problems of low efficiency, high cost, difficult removal of bacteria, protein and glucose, and achieve low viscosity, low cost, and favorable effects of industrial operation

Active Publication Date: 2011-02-23
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low efficiency, high cost, and difficulty in removing bacteria, protein, and glucose in extracting target products from organic acid ferme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the salting-out extraction separation of formic acid

[0013] A formic acid fermentation simulation liquid with a concentration of 20g / L was prepared. Take 4.7g of simulated solution, add 2.8g of dipotassium hydrogen phosphate and 2.5g of methanol, stir evenly, and let it stand at room temperature to form two phases, the upper phase is an alcohol phase rich in formic acid, and the lower phase is a salt phase rich in other components. The distribution coefficient of formic acid is 34.8, and the recovery rate is 98.6%.

Embodiment 2

[0014] Embodiment 2: the salting-out extraction separation of lactic acid

[0015] The lactic acid fermentation broth was filtered through a hollow fiber membrane to obtain a clear filtrate with a lactic acid concentration of 170 g / L. Take 5.6 g of filtrate, add 1.4 g of dipotassium hydrogen phosphate and 3 g of ethanol, stir evenly, and let stand at room temperature to form two phases, the upper phase is an alcohol phase rich in lactic acid, and the lower phase is a salt phase rich in other components. The partition coefficient of lactic acid was 7.0, and the recovery rate was 98.7%.

[0016] Take 5.6g of lactic acid fermentation broth with a concentration of 170g / L, add 1.4g of dipotassium hydrogen phosphate and 3g of ethanol, stir evenly, leave at room temperature to separate phases, the upper phase is the alcohol phase rich in lactic acid, and the lower phase is rich in other ingredients The salt phase, bacteria and protein are between the two phases. The partition coeff...

Embodiment 3

[0020] Embodiment 3: the salting-out extraction separation of acetic acid

[0021] The acetic acid fermentation broth was filtered through a hollow fiber membrane to obtain a clarified filtrate with an acetic acid concentration of 40 g / L. Take 5.6 g of filtrate, add 1.4 g of dipotassium hydrogen phosphate and 3 g of ethanol, stir evenly, and let stand at room temperature to form two phases, the upper phase is an alcohol phase rich in acetic acid, and the lower phase is a salt phase rich in other components. The partition coefficient of acetic acid is 5.4, and the recovery rate is 98.8%.

[0022] Take 5.6 g of acetic acid fermentation broth with a concentration of 40 g / L, add 1.4 g of dipotassium hydrogen phosphate and 3 g of ethanol, the partition coefficient of acetic acid is 6.9, the recovery rate is 98.8%, and the removal rates of protein and bacteria are 81.9% and 100% respectively .

[0023] Acetic acid fermentation simulated liquid with a concentration of 40g / L was pre...

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Abstract

The invention belongs to the technical field of bioengineering, and relates to a method for extracting and salting organic acids out of fermentation liquor. The method is characterized in that: soluble inorganic or organic salt solids or concentrated solution thereof and an extracting agent are added into the organic acid fermentation liquor, stirred for uniform mixing, and stood at room temperature for phase splitting; when two phases are formed by the salting-out and extraction, an upper phase is a solvent phase rich in the organic acids, while a lower phase is a salt phase rich in other components; if the extraction operation is directly performed without pretreating the fermentation liquor, a solid phase layer rich in bacteria and proteins is formed between the two phases; when three phases are formed, the upper phase is the solvent phase, a middle phase is the phase rich in the organic acids and the lower phase is the salt phase rich in the other components; therefore, the aim of extracting and separating the organic acids is fulfilled. The method solves the problems of difficulties in product separation, high cost and the like in the conventional process for separating the organic acids by a fermentation method, is simple, ensures separation time, low cost and high recovery rate, and is an organic acid separation method with industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to the separation technology of microbial fermented liquid, in particular to a method for salting out and extracting organic acids in fermented liquid. Background technique [0002] Organic acids (formic acid, acetic acid, lactic acid, succinic acid, citric acid, malic acid, fumaric acid, adipic acid, long-chain dibasic acids, etc., among which the long-chain dibasic acids are organic acids with more than ten carbon atoms) as a chemical industry with broad production prospects Raw materials and intermediates are widely used in many fields. Among them, acetic acid is mainly used to manufacture polyvinyl acetate and cellulose acetate (also known as cellulose acetate), and can also be used to synthesize various derivatives, such as methyl acetate, ethyl acetate, propyl acetate, butyl acetate, etc. At the same time, acetic acid is also an excellent solvent for paint and paint industry...

Claims

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Application Information

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IPC IPC(8): C07C55/10C07C59/08C07C53/08C07C51/48C07C55/21C07C53/02
CPCC07C51/42C07C51/50C07C51/48C07C53/02C07C53/08C07C55/10C07C55/21C07C59/08
Inventor 修志龙宋志远魏博超孙亚琴
Owner DALIAN UNIV OF TECH
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