Method for extracting and salting organic acids out of fermentation liquor
A fermented liquid and organic acid technology, applied in the field of bioengineering, can solve the problems of low efficiency, high cost, difficult removal of bacteria, protein and glucose, and achieve low viscosity, low cost, and favorable effects of industrial operation
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Embodiment 1
[0012] Embodiment 1: the salting-out extraction separation of formic acid
[0013] A formic acid fermentation simulation liquid with a concentration of 20g / L was prepared. Take 4.7g of simulated solution, add 2.8g of dipotassium hydrogen phosphate and 2.5g of methanol, stir evenly, and let it stand at room temperature to form two phases, the upper phase is an alcohol phase rich in formic acid, and the lower phase is a salt phase rich in other components. The distribution coefficient of formic acid is 34.8, and the recovery rate is 98.6%.
Embodiment 2
[0014] Embodiment 2: the salting-out extraction separation of lactic acid
[0015] The lactic acid fermentation broth was filtered through a hollow fiber membrane to obtain a clear filtrate with a lactic acid concentration of 170 g / L. Take 5.6 g of filtrate, add 1.4 g of dipotassium hydrogen phosphate and 3 g of ethanol, stir evenly, and let stand at room temperature to form two phases, the upper phase is an alcohol phase rich in lactic acid, and the lower phase is a salt phase rich in other components. The partition coefficient of lactic acid was 7.0, and the recovery rate was 98.7%.
[0016] Take 5.6g of lactic acid fermentation broth with a concentration of 170g / L, add 1.4g of dipotassium hydrogen phosphate and 3g of ethanol, stir evenly, leave at room temperature to separate phases, the upper phase is the alcohol phase rich in lactic acid, and the lower phase is rich in other ingredients The salt phase, bacteria and protein are between the two phases. The partition coeff...
Embodiment 3
[0020] Embodiment 3: the salting-out extraction separation of acetic acid
[0021] The acetic acid fermentation broth was filtered through a hollow fiber membrane to obtain a clarified filtrate with an acetic acid concentration of 40 g / L. Take 5.6 g of filtrate, add 1.4 g of dipotassium hydrogen phosphate and 3 g of ethanol, stir evenly, and let stand at room temperature to form two phases, the upper phase is an alcohol phase rich in acetic acid, and the lower phase is a salt phase rich in other components. The partition coefficient of acetic acid is 5.4, and the recovery rate is 98.8%.
[0022] Take 5.6 g of acetic acid fermentation broth with a concentration of 40 g / L, add 1.4 g of dipotassium hydrogen phosphate and 3 g of ethanol, the partition coefficient of acetic acid is 6.9, the recovery rate is 98.8%, and the removal rates of protein and bacteria are 81.9% and 100% respectively .
[0023] Acetic acid fermentation simulated liquid with a concentration of 40g / L was pre...
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