Aqueous enzymatic method for extracting rapeseed oil and recovering protein

A rapeseed oil and protein technology, which is applied in the production, fermentation, and fat production of fat oil/fat, can solve the problems of low oil-containing protein, difficult demulsification and separation, low oil yield, etc., and achieves less environmental pollution and simple process equipment. , The effect of improving oil yield

Inactive Publication Date: 2013-12-11
YANTAI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for extracting rapeseed oil and recovering protein by aqueous enzymatic method, which solves the problems of low oil yield and difficulty in demulsification and separation in the current

Method used

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  • Aqueous enzymatic method for extracting rapeseed oil and recovering protein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Raw material processing: the raw rapeseed is crushed into rapeseed cake according to the conventional method, and the citric acid solution with pH 4.5-5.5 is soaked at high temperature (80-120℃) for 0.1-2h, and then the solid-liquid ratio is 1:2-1 :20 Replenish citric acid solution, refining, plate and frame filtration to separate and remove the crust. The material liquid is cooled to 30-60°C.

[0028] 2) Enzymatic hydrolysis: add 50-500U / g composite cellulase according to the dry weight of rapeseed, enzymatically hydrolyze at 30-60℃ for 2-6h, then add 100-500U / g acid protease, enzymatically hydrolyze at 30-60℃ 2- 6h. After the enzymolysis is completed, the temperature is raised to 80-120°C and kept for 5-10min to inactivate the enzyme.

[0029] 3) Residue separation: the enzymatic hydrolysis solution is separated by a horizontal centrifuge to obtain liquid phase 1 and the residue.

[0030] 4) Demulsification: Add concentrated sulfuric acid to liquid phase 1 to adjust th...

Embodiment 2

[0034] 1) Raw material processing: The raw rapeseed is crushed into rapeseed cake according to the conventional method, and the acetic acid solution with pH 4.5-5.5 is soaked at high temperature (80-120℃) for 0.1-2h, and then the solid-liquid ratio is 1:2-1: 20 Supplement acetic acid, refining, plate and frame filtration to separate and remove the skin. The material liquid is cooled to 30-60°C.

[0035] 2) Enzymatic hydrolysis: add 50-500U / g composite cellulase according to the dry weight of rapeseed, enzymatically hydrolyze at 30-60℃ for 2-6h, then add 100-500U / g neutral protease and xylanase composite enzyme , Enzymatic hydrolysis at 30-60℃ for 2-6h. After the enzymolysis is completed, the temperature is raised to 80-120°C and kept for 5-10min to inactivate the enzyme.

[0036] 3) Residue separation: the enzymatic hydrolysis solution is separated by a horizontal centrifuge to obtain liquid phase 1 and the residue.

[0037] 4) Demulsification: Add sodium chloride solid to liquid ...

Embodiment 3

[0041] 1) Raw material processing: The raw rapeseed is crushed into rapeseed cake according to the conventional method, and soaked in 0.01M phosphoric acid solution at high temperature (80-120℃) for 0.1-2h, and then supplemented with phosphoric acid according to the solid-liquid ratio 1:2-1:20 Solution, refining, plate and frame filtration to separate and remove the skin shell. The material liquid is cooled to 30-60°C.

[0042] 2) Enzymatic hydrolysis: add 50-500U / g composite cellulase according to the dry weight of rapeseed, enzymatically hydrolyze at 30-60℃ for 2-6h, then add 100-500U / g trypsin and pectinase composite enzyme, 30 Enzymatic hydrolysis at -60℃ for 2-6h. After the enzymolysis is completed, the temperature is raised to 80-120°C and kept for 5-10min to inactivate the enzyme.

[0043] 3) Residue separation: the enzymatic hydrolysis solution is separated by a horizontal centrifuge to obtain liquid phase 1 and the residue.

[0044] 4) Demulsification: Add polyacrylamide ...

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Abstract

The invention relates to an aqueous enzymatic method for extracting rapeseed oil and recovering protein. The aqueous enzymatic method comprises following steps: raw material rapeseed is crushed, immersed in an acid solution at a high temperature (80 to 120 DEG C) for 0.1 to 2h, and grinded to obtain a pulp; the pulp is filtered so as to remove shells; the filtrate is subjected to enzymolysis for 2 to 6h at a temperature of 30 to 60 DEG C by adding 50-500U/g of composite cellulose, then 100-500U/g of protease and a composite enzyme of protease is added, and the mixture is subjected to enzymolysis for another 2 to 6h at a temperature of 30 to 60 DEG C; the mixture is filtered, the obtained residue can be used as forage directly after drying, and the obtained liquid phase is subjected to emulsion breaking, wherein emulsion breaking is realized by three methods; after emulsion breaking, the liquid phase is filtered to obtain liquid content and solid content; the solid content is subjected to spray drying so as to obtain rapeseed protein; and the liquid content is filtered so as to obtain rapeseed oil and a water phase, and the water phase is treated with a membrane so as to obtain soluble protein. The three methods of emulsion breaking comprise: A, a food flocculating agent is added for emulsion breaking; B, concentrated sulfuric acid or sodium hydroxide solution is added to adjust the pH value to the isoelectric point of the liquid phase for emulsion breaking; and C, sodium chloride solid is added for salting out emulsion breaking. Recovery rate and quality of the rapeseed oil and protein obtained by the aqueous enzymatic method are high, and no organic solvent is used in the whole process of the aqueous enzymatic method. Rapeseed is immersed in the acid solution before enzymolysis, so that the generation of antinutritional factors is avoided. Processing equipment required by the aqueous enzymatic method is simple, and cost is low.

Description

(1) Technical field [0001] The invention relates to a method for extracting rapeseed oil and recovering protein by an aqueous enzymatic method, belonging to a vegetable oil and vegetable protein extraction process in agricultural product processing, and is suitable for extracting rapeseed oil and low-denaturation and low-oil-containing protein from rapeseed. (2) Background technology [0002] At present, the commonly used methods for producing oil in the vegetable oil industry include squeezing method, pre-expression leaching method, and aqueous method. The squeezing method and the pre-squeezing leaching method use mechanical methods to destroy the cell wall, use damp heat to destroy the cell membrane and denature the protein, so that the oil can be smoothly squeezed or extracted. The pressing method has less supporting equipment and simple process, which can keep the original special flavor of the product, but this method has a high residual oil rate (up to 7-10%), high cost, hi...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04C12P21/06
Inventor 姜爱莉单守水鞠宝林剑
Owner YANTAI UNIV
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