Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent

A collagen and vascular stent technology, applied in the directions of blood vessels, stents, anticoagulation treatment, etc., can solve the problems of unfavorable smooth muscle cell growth around the axis, unfavorable three-dimensional osmotic growth of smooth muscle cells, small nano/micro fiber pore size, etc. Promotes periaxial growth and three-dimensional migration, excellent mechanical properties and biocompatibility, and inhibits smooth muscle cell proliferation

Active Publication Date: 2016-01-13
DONGHUA UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if dense and random nanofibers are used for the outer layer of vascular stents, there are many disadvantages: first, the nano/micro fibers obtained by traditional electrospinning have a small pore size, which is not conducive to the

Method used

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  • Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent
  • Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent
  • Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent

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Embodiment 1

[0039] Weigh 0.9 g of P(LLA-CL) and 0.3 g of collagen with an electronic balance, dissolve them in 10 mL of hexafluoroisopropanol, stir overnight until completely dissolved, and obtain P(LLA-CL) / collagen composite spinning solution; 0.3g heparin sodium and 20 μg VEGF were dissolved in 0.2mL diluent to obtain the drug solution loaded on the inner layer; 20 μg PDGF was dissolved in 0.3mL diluent to obtain the drug solution loaded on the outer layer, wherein the diluent had a pH of 7.2-7.4 PBS buffer solution and 0.1% bovine serum albumin BSA; prepare the inner layer of the blood vessel by coaxial electrospinning, the spinning parameters are as follows: the electrostatic high voltage is 16kV, the shell solution (P(LLA-CL) / collagen Protein composite spinning solution) advancing speed is 1.0mL / h, core layer solution (inner layer loaded drug solution) advancing speed is 0.1mL / h, receiving distance is 12cm, stainless steel rod rotation speed is 1000rpm; spinning is carried out 2 hours...

Embodiment 2

[0041] Weigh 0.75g of P(LLA-CL) and 0.25g of collagen with an electronic balance, dissolve them in 10mL of hexafluoroisopropanol, stir overnight until completely dissolved, and obtain P(LLA-CL) / collagen composite spinning solution; 0.75g heparin sodium and 20 μg VEGF were dissolved in 0.5mL diluent to obtain a drug solution loaded on the inner layer; 20 μg PDGF was dissolved in 0.5mL diluent to obtain a drug solution loaded on the outer layer, wherein the diluent had a pH of 7.2-7.4 PBS buffer and 0.1% bovine serum albumin BSA; prepare the inner layer of the tube by coaxial electrospinning, the spinning parameters are as follows: the electrostatic high voltage is 14kV, the shell solution (P(LLA-CL) / collagen Composite spinning solution) advancing speed is 1.0mL / h, core layer solution (inner layer loaded drug solution) advancing speed is 0.1mL / h, receiving distance is 12cm, stainless steel rod rotation speed is 1200rpm; spinning carries out after 5 hours , using the bidirectiona...

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Abstract

The invention relates to a preparation method of a heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent. The method comprises the following steps: uniformly mixing P(LLA-CL) with collagen in a solvent to obtain a composite spinning solution; dissolving heparin sodium and VEGF in a dilution solution to obtain an internal layer supported medicine solution; dissolving PDGF in the dilution solution to obtain an external layer supported medicine solution; carrying out coaxial electrostatic spinning with the internal layer supported medicine solution as a core layer and the spinning solution as a shell layer to obtain a intravascular stent internal layer; and carrying out bidirectional conjugate electrostatic spinning with the external layer loaded medicine solution as a core layer and the spinning solution as a shell layer to obtain an intravascular stent external layer, continuously receiving the intravascular stent external layer at the outer side of the intravascular stent internal layer to obtain a bilayer intravascular stent, and cross-linking to obtain the heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent. The intravascular stent provided by the invention has excellent mechanical performances and biocompatibility, has natural blood vessel simulating components, structure and functions, is in favor of realizing in situ regeneration of blood vessel tissues and reconstruction of a multilayer structure, and has important applications in the blood vessel tissue engineering.

Description

technical field [0001] The invention belongs to the field of preparation methods of double-layer vascular stents, and in particular relates to a preparation method of P(LLA-CL) / collagen double-layer vascular stents coordinated and regulated by heparin and geminifactors. Background technique [0002] The application of tissue engineered vascular stents should mainly meet the following requirements: First, vascular stents should have excellent biomechanical properties and vascular compliance, as well as excellent biocompatibility, to meet the support and histocompatibility of the vascular body structure Secondly, the lumen of the vascular stent should have long-term antithrombotic function, avoid the intimal hyperplasia of smooth muscle cells, accelerate endothelialization, and maintain the unobstructed blood flow; at the same time, the outer layer of the stent should promote the smooth muscle cell wrapping. Axial alignment and three-dimensional penetration can regulate the ph...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/24A61L27/18A61L27/54A61L27/58A61L33/10D04H1/728D04H1/76D04H1/4382D01D5/00A61F2/07
Inventor 莫秀梅吴桐王元非
Owner DONGHUA UNIV
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