Preparation method of heparin and twin factor synergistically regulated P(LLA-CL)/collagen bilayer intravascular stent

Abstract

The invention relates to a preparation method of a heparin and twin factor synergistically regulated P(LLA-CL) / collagen bilayer intravascular stent. The method comprises the following steps: uniformly mixing P(LLA-CL) with collagen in a solvent to obtain a composite spinning solution; dissolving heparin sodium and VEGF in a dilution solution to obtain an internal layer supported medicine solution; dissolving PDGF in the dilution solution to obtain an external layer supported medicine solution; carrying out coaxial electrostatic spinning with the internal layer supported medicine solution as a core layer and the spinning solution as a shell layer to obtain a intravascular stent internal layer; and carrying out bidirectional conjugate electrostatic spinning with the external layer loaded medicine solution as a core layer and the spinning solution as a shell layer to obtain an intravascular stent external layer, continuously receiving the intravascular stent external layer at the outer side of the intravascular stent internal layer to obtain a bilayer intravascular stent, and cross-linking to obtain the heparin and twin factor synergistically regulated P(LLA-CL) / collagen bilayer intravascular stent. The intravascular stent provided by the invention has excellent mechanical performances and biocompatibility, has natural blood vessel simulating components, structure and functions, is in favor of realizing in situ regeneration of blood vessel tissues and reconstruction of a multilayer structure, and has important applications in the blood vessel tissue engineering.

Description

technical field [0001] The invention belongs to the field of preparation methods of double-layer vascular stents, and in particular relates to a preparation method of P(LLA-CL) / collagen double-layer vascular stents coordinated and regulated by heparin and geminifactors. Background technique [0002] The application of tissue engineered vascular stents should mainly meet the following requirements: First, vascular stents should have excellent biomechanical properties and vascular compliance, as well as excellent biocompatibility, to meet the support and histocompatibility of the vascular body structure Secondly, the lumen of the vascular stent should have long-term antithrombotic function, avoid the intimal hyperplasia of smooth muscle cells, accelerate endothelialization, and maintain the unobstructed blood flow; at the same time, the outer layer of the stent should promote the smooth muscle cell wrapping. Axial alignment and three-dimensional penetration can regulate the ph...

AI Technical Summary

Problems solved by technology

However, if dense and random nanofibers are used for the outer layer of vascular stents, there are many disadvantages: first, the nano / micro fibers obtained by traditional electrospinning have a small pore size, which is not conducive to the three-dimensional osmotic growth of smooth muscle cells; secondly, random Nanofibers are not conducive to inducing the growth of smooth muscle cells around the axis, and cannot achieve the effect of bionic natural vascular smooth muscle layer

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