Method for extracting heparin sodium by utilizing pork lungs

Abstract

The invention relates to a method for extracting heparin sodium by utilizing pork lungs, which has the following steps of: grinding fresh pork lungs into pork lung paste; adding deionized water, lysis agent and preservative; reacting to obtain pork lung serous fluid; adding deionized water and sodium chloride; reacting to obtain pork lung alkaline hydrolysis liquid; slowly heating the pork lung alkaline hydrolysis liquid and adding heparin sodium protamex; after heat preservation and reaction, slowly heating and continuously carrying out heat preservation to obtain pork lung enzymolysis liquid; replenishing sodium chloride after cooling the pork lung enzymolysis liquid; reacting to obtain pork lung salt hydrolysis liquid; heating and carrying out heat preservation on the pork lung salt hydrolysis liquid; adding composite protein precipitation agent; stirring; collecting clear liquid after static placing; concentrating the clear liquid and then adding ethanol; precipitating overnight to obtain precipitate; and dehydrating and drying to obtain a crude product of the heparin sodium. According to the invention, the production cost of the heparin sodium can be reduced, the product quality and the yield can be improved, the large-scale production can be easily realized, meanwhile, the usage amount of chemical reagents is reduced, the emission of waste is reduced, and no waste gas orwaste water is discharged. The economic benefit and the social benefit are obvious.

Description

technical field [0001] The invention relates to the technical field of biopharmaceutical production, in particular to a method for extracting heparin sodium from pig lungs. Background technique [0002] Heparin sodium has the function of preventing blood coagulation and dissolving thrombus. It is an excellent anticoagulant drug. Since its discovery in the early 20th century, it has been widely used in the production of cardiovascular and cerebrovascular drugs and in the cosmetics industry. European and American countries have extracted a large amount of heparin sodium from bovine lungs and sheep livers, but later due to the prevalence of mad cow (sheep) disease, and years of experiments have shown that only heparin extracted from healthy pig organs has the same molecular structure as the heparin in the human body. Consistently, the pig small intestine gradually became the main source of heparin. [0003] At present, the crude heparin sodium production in my country mostly u...

AI Technical Summary

Problems solved by technology

The traditional process includes tissue homogenization and autolysis, extraction of heparin and lipoprotein complexes with alkaline ammonium sulfate or sodium chloride, ethanol degreasing, enzymatic hydrolysis and precipitation, etc. The traditional process is cumbersome, and the prepared heparin has low grade and light color Yellow, low potency (less than 20u / mg), low product yield, difficult to guarantee quality, low production efficiency and unable to scale production

[0005] Some domestic institutions are also researching the extraction of porcine lung heparin, but because the extraction rate of the product is extremely low (the extraction rate per kilogram of porcine lung slurry is less than 15,000 international units), the cost is high and it does not have industrial value, and it contains many heparins, etc. Impurities such as polysulfides and proteins make it difficult for the product quality to meet the sales standard and cannot be produced on a large scale