Nanofiber vascular prostheses and preparation method

A technology of nanofibers and artificial blood vessels, applied in fiber processing, spinning solution preparation, medical science, etc., can solve the problems of unfavorable gelatin membranes, achieve the effects of preventing blood penetration, improving physical and mechanical properties, and promoting endothelialization

Active Publication Date: 2010-05-19
南通双辉医疗器械科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gelatin film shrinks and curls violently after cross-linking, which is not conducive to obtaining a flat gelatin film, and this problem has not been effectively solved

Method used

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  • Nanofiber vascular prostheses and preparation method
  • Nanofiber vascular prostheses and preparation method
  • Nanofiber vascular prostheses and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A nanofiber artificial blood vessel is made by the following method:

[0039] (1) dissolving gelatin in glacial acetic acid to prepare 20 mL of gelatin solution with a mass fraction of 15%;

[0040] (2) Add 1 mL of an aqueous solution of glutaraldehyde with a mass percentage concentration of 50% and mix well to make it moderately pre-crosslinked, and add 5 mL of an aqueous solution of heparin sodium with a mass concentration of 0.9% to form a gelatin-heparin sodium spinning solution. Silk stock solution; the spinning stock solution is loaded into the electrospinning equipment, and the electrospinning process is adopted (process parameters: voltage 15kV, receiving distance 15cm, solution flow rate 0.6mL / h, collection roller speed 100r / min, needle No. 5 needle), the fibers ejected and formed from the spinning nozzle in the electrospinning equipment are collected on the collection roller, and the electrospinning is continued for 5 hours to form a nanofiber non-woven film t...

Embodiment 2

[0044] A nanofiber artificial blood vessel is made by the following method:

[0045] (1) dissolving gelatin in glacial acetic acid to prepare 20 mL of gelatin solution with a mass fraction of 15%;

[0046] (2) Add 1 mL of an aqueous solution of genipin with a concentration of 10% by mass and mix well to make it moderately pre-crosslinked, and add 5 mL of an aqueous solution of sodium heparin with a concentration of 1.5% to form gelatin-sodium heparin spinning Silk stock solution; the spinning stock solution is loaded into the electrospinning equipment, and the electrospinning process is adopted (process parameters: voltage 15kV, receiving distance 15cm, solution flow rate 0.5mL / h, collection roller speed 100r / min, needle No. 5 needle), continuous electrospinning for 5h, the fibers ejected and formed from the spinning nozzle in the electrospinning equipment are collected on the collection roller, and the nanofiber non-woven film tube is formed on the collection roller, and the ...

Embodiment 3

[0050] A nanofiber artificial blood vessel is made by the following method:

[0051] (1) dissolving collagen in glacial acetic acid to prepare 20 mL of gelatin solution with a mass fraction of 15%;

[0052] (2) Add 0.001 mL of an aqueous carbodiimide solution with a mass percentage concentration of 60% and mix well to make it moderately pre-crosslinked, and add 5 mL of a 3% heparin sodium aqueous solution to form collagen-heparin Sodium spinning stock solution; the spinning stock solution is loaded into the electrospinning equipment, and the electrospinning process is adopted (process parameters: voltage 15kV, receiving distance 15cm, solution flow rate 0.5mL / h, collection roller speed 100r / min, needle No. 5 needle), continuous electrospinning for 5h, the fibers ejected and formed by the spinning nozzle in the electrospinning equipment are collected on the collection roller, and the nanofiber non-woven film tube is formed on the collection roller, the shaft of the collection r...

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Abstract

The invention discloses a nanofiber vascular prostheses and a preparation method. The preparation method is as follows: mixing the solution of gelatin and glacial acetic acid with the aqueous solution of a crosslinker evenly to pre-crosslink the solution of gelatin and glacial acetic acid, adding heparin sodium aqueous solution to prepare spinning dope and collecting the formed fibers on a collecting roll by using the electrospinning process to form a nanofiber nonwoven membrane tube; dissolving polyurethane into the mixed solvent of tetrahydrofuran and N,N-dimethylformamide to prepare polyurethane spinning dope and continuously collecting the formed fibers on the collecting roll which has collected the nanofiber nonwoven membrane tube by using the electrospinning process; and taking off the nanofiber nonwoven membrane tube covered by the polyurethane fiber nonwoven membrane tube structure from the collecting roll and then soaking the nanofiber nonwoven membrane tube into the aqueous solution of a post-crosslinker to carry out post-crosslinking treatment, thus preparing the nanofiber vascular prostheses. The inner layer of the vascular prostheses of the invention can improve the blood compatibility and the outer layer has biological stability and can improve the physical and mechanical properties.

Description

technical field [0001] The invention relates to an artificial blood vessel and a preparation method, in particular to a nanofiber artificial blood vessel and a preparation method. Background technique [0002] Due to the poor self-repair ability of human blood vessels after serious injury, it cannot be quickly recovered, so artificial blood vessels have attracted more and more attention in recent years. So far, people have developed artificial blood vessels made of various materials, such as polyurethane, polylactic acid, polytetrafluoroethylene, nylon, etc. These materials have certain biocompatibility, but they cannot meet the needs of artificial blood vessels. [0003] With the development of technology, people began to use natural biopolymers to modify the inner layer of these materials to construct an environment for endothelial cell adhesion and growth to improve biocompatibility. Natural biopolymers have good biocompatibility and often exhibit biological activities s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/24A61L27/22A61L27/18D01D1/02D01D5/00
Inventor 冯亚凯肖若芳郭锦棠赵海洋孟繁茹
Owner 南通双辉医疗器械科技有限公司
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