Method for breaking dormancy of millet for breeding test

Abstract

The invention relates to the technical field of breeding, in particular to a millet dormancy breaking method for a breeding test, which comprises the following steps: soaking disinfected ears in a 0.1-0.3 mol / L KOH solution for 10-15 minutes, then washing with sterile water, fishing out, and airing surface moisture to obtain softened ears. Soaking the softened ears into an activating solution containing a composite abscisic acid removing agent, heating the activating solution to 25-40 DEG C in a water bath, and treating for 12-48 hours under the condition that the illumination intensity is 2000-3000Lx; wherein the composite abscisic acid removing agent is composed of oxalyl, edible torula yeast and gibberellin according to the mass ratio of (5-7): (2-3): (1-2), a solvent of the activating solution is sterile water with the agar mass content being 2-5%, and the ratio of the composite abscisic acid removing agent to the solvent is 1-3 g / L. Peat soil is fully wetted with sterile water, it is guaranteed that water does not drip after the wetted peat soil is held by hands to form clusters, a prefabricated substrate is obtained, the activated ears are buried in the prefabricated substrate to be cultured for 24 hours, and millet dormancy is fully broken.

Description

Technical Field

[0001] The invention belongs to the technical field of breeding, and in particular relates to a method for breaking millet dormancy for breeding experiments. Background Art

[0002] Millet is a plant belonging to the Gramineae family. It was called millet, millet, and sorghum in ancient times. It is an annual herb; the stalk is thick and has few tillers, and the narrow lanceolate leaves have obvious midrib and veinlets, and fine hairs; the panicle inflorescence is spike-shaped; the ear is 20 to 30 cm long; the spikelets grow in clusters on the tertiary stalks, and the spikelets are basically covered with prickles. Each ear has hundreds to thousands of seeds, which are very small, about 0.1 cm in diameter. The ears are generally golden yellow when mature, and the seeds are oval, and the grains are small and mostly yellow.

[0003] Seed dormancy refers to the state or phenomenon in which viable seeds do not germinate under suitable germination conditions. Seed dormancy is very common in plants and is a strategy for plants to adapt to adversity and protect species continuation during their long-term phylogenetic development. Conventional millet breeding methods include screening core germplasm, configuring hybrid combinations, and systematic breeding. If the dormancy of millet seeds can be fully broken before millet breeding, the efficiency of breeding experiments can be greatly increased.

[0004] The existing methods of breaking millet dormancy mostly use warm water soaking or soaking after adding an appropriate amount of plant growth hormone. However, this traditional method cannot effectively inhibit the growth-inhibiting plant hormones such as abscisic acid in the millet seeds, resulting in the inability to fully break the millet dormancy, and thus the effect is not good. Summary of the invention

[0005] In view of the deficiencies in the above-mentioned background technology, the present invention provides a method for breaking millet dormancy for breeding experiments, which can fully inhibit the growth inhibitory hormone abscisic acid in millet seeds, so that the dormancy of millet is fully broken in a short time. Specifically: The present invention provides a method for breaking millet dormancy for breeding experiments, comprising the following steps: S1: immersing the pretreated ears of grain into an activation solution for activation treatment, wherein the activation solution contains a composite abscisic acid remover; S2: After the activated ears of millet are buried in a prefabricated matrix for a period of time, the dormancy of the millet is fully broken.

[0006] As a further illustration of the present invention, the pretreatment process includes disinfection and softening of the ears of grain.

[0007] As a further illustration of the present invention, the specific process of disinfecting the ears of grain is as follows: soaking the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 1-3% for 15 to 20 minutes, then washing them with sterile water, and then taking them out and drying the surface moisture to obtain the disinfected ears of grain.

[0008] As a further illustration of the present invention, the specific softening process of the ears of grain is as follows: the ears of grain are immersed in a 0.1-0.3 mol / L KOH solution for 10-15 min, then rinsed with sterile water, and taken out and dried to obtain the softened ears of grain.

[0009] As a further illustration of the present invention, the composite abscisic acid remover is composed of oxalyl, edible torula yeast and gibberellin, wherein the mass ratio of oxalyl, edible torula yeast and gibberellin is 5-7:2-3:1-2.

[0010] As a further illustration of the present invention, the solvent of the activation solution is sterile water with an agar content of 2-5% by mass, and the ratio of the composite abscisic acid release agent to the solvent is 1-3 g / L.

[0011] As a further illustration of the present invention, the activation treatment conditions are treatment for 12 to 48 hours at a water temperature of 25-40°C and a light intensity of 2000 to 3000 Lx.

[0012] As a further illustration of the present invention, the prefabricated matrix is ​​obtained by fully moistening the peat soil with sterile water to ensure that the moistened peat soil does not drip water after being grasped into a ball.

[0013] As a further illustration of the present invention, the ears of grain are buried in the prefabricated matrix for 24 hours.

[0014] Compared with the prior art, the present invention has the following advantages: The invention adopts low-concentration alkali solution to alkali-etch the seed coat, so as to soften the soft seed coat and increase its water permeability, so that the activation liquid can quickly enter the seed coat and quickly contact the seed and the organic matter inside the seed coat, so as to play a role more quickly.

[0015] The composite abscisic acid release agent of the present invention can play a role in a short time, so as to achieve the purpose of inhibiting plant endogenous hormones such as "dormancy hormone" (abscisic acid) and the like. The physiological function of abscisic acid is to accelerate seed dormancy and inhibit its germination. Therefore, the present invention can quickly break the seed dormancy by removing the inhibitory effect of abscisic acid in the seeds. Specifically, oxalyl in the activation solution is the main abscisic acid release agent, which can significantly improve the germination rate of seeds inhibited by abscisic acid. Gibberellic acid belongs to a plant growth regulator, which can accelerate seed germination and synergize with oxalyl to further remove substances in the seeds that inhibit seed embryo growth, such as abscisic acid and ethylene, so as to promote the seed embryo to change from a dormant state to a growing state. Due to the action of active substances such as gibberellins and oxalyl in the activation solution and the growth hormone in the seeds, the oxygen available to the seeds in the activation solution is consumed. Due to the presence of the outer skin in the rice ear, the oxygen with too low a concentration cannot quickly enter the seed skin, so that the seeds are easily hypoxic. Therefore, a trace amount of edible round yeast is added to the activation solution, which quickly plays a role in warm water and can replenish the dissolved oxygen in the activation solution in time.

[0016] The invention buries the activated ears of millet in a peat soil matrix to further germinate the seed embryos, thereby fully breaking the dormancy of the millet. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A flow chart of a method for breaking millet dormancy for breeding experiments provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0018] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] The existing methods of breaking millet dormancy mostly use warm water soaking or soaking after adding an appropriate amount of plant growth hormone. However, this traditional method cannot effectively inhibit the growth-inhibiting plant hormones such as abscisic acid in the millet seeds, resulting in the dormancy of the millet cannot be fully broken, resulting in poor results.

[0020] The present invention provides a method for breaking millet dormancy for breeding experiments, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 1-3% for 15-20 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0021] Among them, the mass concentration of the sodium hypochlorite aqueous solution is 1%, 2%, 3% and the like; the immersion time is 15min, 16min, 17min, 18min, 20min and the like.

[0022] S2: Soak the sterilized ears of grain in a 0.1-0.3 mol / L KOH solution for 10-15 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0023] Among them, the concentration of KOH solution is 0.1mol / L, 0.2mol / L, 0.3mol / L, etc.; the immersion time is 10min, 11min, 12min, 13min, 15min, etc.

[0024] The invention adopts low-concentration alkali solution to alkali-etch the seed coat, so as to soften the soft seed coat and increase its water permeability, so that the activation liquid can quickly enter the seed coat and quickly contact the seed and the organic matter inside the seed coat, so as to play a role more quickly.

[0025] S3: Immerse the softened ears of grain in an activation solution containing a composite abscisic acid release agent, heat the activation solution in a water bath to 25-40°C, and treat for 12-48 hours under a light intensity of 2000-3000Lx; wherein the composite abscisic acid release agent is composed of oxalyl, edible round yeast and gibberellin in a mass ratio of 5-7:2-3:1-2, the solvent of the activation solution is sterile water with an agar mass content of 2-5%, and the ratio of the composite abscisic acid release agent to the solvent is 1-3g / L.

[0026] The composite abscisic acid release agent of the present invention can play a role in a short time, so as to achieve the purpose of inhibiting plant endogenous hormones such as "dormancy hormone" (abscisic acid) and the like. The physiological function of abscisic acid is to accelerate seed dormancy and inhibit its germination. Therefore, the present invention can quickly break the seed dormancy by removing the inhibitory effect of abscisic acid in the seeds. Specifically, oxalyl in the activation solution is the main abscisic acid release agent, which can significantly improve the germination rate of seeds inhibited by abscisic acid. Gibberellic acid belongs to a plant growth regulator, which can accelerate seed germination and synergize with oxalyl to further remove substances in the seeds that inhibit seed embryo growth, such as abscisic acid and ethylene, so as to promote the seed embryo to change from a dormant state to a growing state. Due to the action of active substances such as gibberellins and oxalyl in the activation solution and the growth hormone in the seeds, the oxygen available to the seeds in the activation solution is consumed. Due to the presence of the outer skin in the rice ear, the oxygen with too low a concentration cannot quickly enter the seed skin, so that the seeds are easily hypoxic. Therefore, a trace amount of edible round yeast is added to the activation solution, which quickly plays a role in warm water and can replenish the dissolved oxygen in the activation solution in time.

[0027] The temperature of the activation solution in the present invention is preferably 30-35°C. This temperature condition can accelerate the active substances in the composite abscisic acid release agent to take effect, accelerate its contact with the hormone substances inside the seeds, and will not destroy the activity of the composite abscisic acid release agent. The temperature of the activation solution can be, for example, 30°C, 31°C, 32°C, 33°C, 34°C, 35°C, etc.

[0028] Since oxalyl is a small molecule released by bacteria and edible round yeast is a yeast, 2-5% agar nutrients are added to the activation solution to accelerate the activation of fungi. The mass content of agar in the solvent can be, for example, 2%, 3%, 4%, 5%, etc.

[0029] The light intensity can be, for example, 2000Lx, 2300Lx, 2500Lx, 2700Lx, 3000Lx, etc.; the activation treatment time can be, for example, 12 hours, 18 hours, 24 hours, 30 hours, 36 hours, 42 hours, 48 ​​hours, etc. The mass ratio of oxalyl, edible round yeast and gibberellin can be, for example, 5:3:2, 6:3:1, 6:2:2, 7:2:1, etc.; the ratio of the composite abscisic acid remover to the solvent can be 1g / L, 2g / L, 3g / L, etc.

[0030] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet.

[0031] The invention buries the activated ears of millet in a peat soil matrix to further germinate the seed embryos, thereby fully breaking the dormancy of the millet.

[0032] The following is explained in conjunction with specific embodiments: Example 1

[0033] This embodiment provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 1% for 15 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0034] S2: Soak the sterilized ears of grain in a 0.1 mol / L KOH solution for 10 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0035] S3: Immerse the softened ears of grain in an activation solution containing a composite abscisic acid release agent, heat the activation solution to 25°C in a water bath, and then treat it for 12 hours under a light intensity of 2000Lx; wherein the composite abscisic acid release agent is composed of oxalyl, edible round yeast and gibberellin in a mass ratio of 5:3:2, the solvent of the activation solution is sterile water with a 2% agar mass content, and the ratio of the composite abscisic acid release agent to the solvent is 1g / L.

[0036] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet. Example 2

[0037] This embodiment provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 2% for 17 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0038] S2: Soak the sterilized ears of grain in a 0.2 mol / L KOH solution for 12 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0039] S3: Immerse the softened ears of grain in an activation solution containing a composite abscisic acid release agent, heat the activation solution to 30°C in a water bath, and then treat it for 24 hours under a light intensity of 2500Lx; wherein the composite abscisic acid release agent is composed of oxalyl, edible round yeast and gibberellin in a mass ratio of 6:2:2, the solvent of the activation solution is sterile water with a 3% agar mass content, and the ratio of the composite abscisic acid release agent to the solvent is 2g / L.

[0040] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet. Example 3

[0041] This embodiment provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 2% for 20 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0042] S2: Soak the sterilized ears of grain in a 0.2 mol / L KOH solution for 15 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0043] S3: Immerse the softened ears of grain in an activation solution containing a composite abscisic acid release agent, heat the activation solution to 35°C in a water bath, and then treat it for 24 hours under a light intensity of 2500Lx; wherein the composite abscisic acid release agent is composed of oxalyl, edible round yeast and gibberellin in a mass ratio of 7:2:1, the solvent of the activation solution is sterile water with a 3% agar mass content, and the ratio of the composite abscisic acid release agent to the solvent is 2g / L.

[0044] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet. Example 4

[0045] This embodiment provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 3% for 20 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0046] S2: Soak the sterilized ears of grain in a 0.3 mol / L KOH solution for 15 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0047] S3: Immerse the softened ears of grain in an activation solution containing a composite abscisic acid release agent, heat the activation solution to 40°C in a water bath, and then treat it for 48 hours under a light intensity of 3000Lx; wherein the composite abscisic acid release agent is composed of oxalyl, edible round yeast and gibberellin in a mass ratio of 6:3:1, the solvent of the activation solution is sterile water with a 5% agar mass content, and the ratio of the composite abscisic acid release agent to the solvent is 3g / L.

[0048] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet.

[0049] Comparative Example 1 This comparative example provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 2% for 20 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0050] S3: Immerse the disinfected ears of grain in an activation solution containing a composite abscisic acid remover, heat the activation solution to 35°C in a water bath, and then treat it for 24 hours under a light intensity of 2500Lx; wherein, the composite abscisic acid remover is composed of oxalyl, edible round yeast and gibberellin in a mass ratio of 7:2:1, the solvent of the activation solution is sterile water with a 3% agar mass content, and the ratio of the composite abscisic acid remover to the solvent is 2g / L.

[0051] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet.

[0052] Comparative Example 2 This comparative example provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 2% for 20 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0053] S2: Soak the sterilized ears of grain in a 0.2 mol / L KOH solution for 15 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0054] S3: Immerse the softened ears of grain in an activation solution containing gibberellin, heat the activation solution to 35°C in a water bath, and treat it for 24 hours under a light intensity of 2500Lx; wherein the ratio of gibberellin to solvent water is 0.2g / L.

[0055] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet.

[0056] Comparative Example 3 This comparative example provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 2% for 20 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0057] S2: Soak the sterilized ears of grain in a 0.2 mol / L KOH solution for 15 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0058] S3: Immerse the softened ears of grain in an activation solution containing a composite abscisic acid release agent, heat the activation solution to 35°C in a water bath, and then treat it for 24 hours under a light intensity of 2500Lx; wherein, the composite abscisic acid release agent is composed of oxalyl and gibberellin in a mass ratio of 7:3, the solvent of the activation solution is sterile water with a 3% agar mass content, and the ratio of the composite abscisic acid release agent to the solvent is 2g / L.

[0059] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet.

[0060] Comparative Example 4 This comparative example provides a method for breaking millet dormancy, comprising the following steps: S1: Soak the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 2% for 20 minutes, then rinse them with sterile water, and take them out and dry the surface moisture to obtain the sterilized ears of grain.

[0061] S2: Soak the sterilized ears of grain in a 0.2 mol / L KOH solution for 15 min, then rinse with sterile water, fish them out and dry the surface moisture to obtain the softened ears of grain.

[0062] S3: Immerse the softened ears of grain in an activation solution containing a composite abscisic acid release agent, heat the activation solution to 35°C in a water bath, and then treat it for 24 hours under a light intensity of 2500Lx; wherein, the composite abscisic acid release agent is composed of edible round yeast and gibberellin in a mass ratio of 2:1, the solvent of the activation solution is sterile water with an agar mass content of 3%, and the ratio of the composite abscisic acid release agent to the solvent is 0.5g / L.

[0063] S4: Use sterile water to fully moisten the peat soil, and ensure that the moistened peat soil does not drip water after being grasped in the hand to obtain a prefabricated matrix. Bury the activated ears of millet in the prefabricated matrix and culture them for 24 hours to fully break the dormancy of millet.

[0064] The germination potential and germination rate of the grains obtained in the above embodiments and comparative examples were statistically analyzed: The millet seeds obtained by the methods provided in Examples 2-3 and Comparative Examples 1-4 were treated using GBT5520-2011—Grain and Oil Inspection Germination Test Standard to determine the germination potential and germination rate of millet: The test method is as follows: Seeds were randomly selected from intact grain seeds that had been inspected for purity, and 100 seeds were placed in a group. The grain seeds were placed on filter paper germination beds according to the groups. After covering, the germination beds were placed in a constant temperature germination box at 30°C for germination tests.

[0065] After the germination test begins, in addition to maintaining the moisture and temperature required for germination, check the germination situation once a day. According to the specified deadlines for germination potential and germination rate, check the normal and abnormal germinated grains in time and keep records. The germination potential days are 3 days, and the germination rate days are 7 days.

[0066] Germination potential (X) and germination rate (Y) are calculated according to formula (1) and formula (2), respectively: (1) (2) In the formula: X is the seed germination potential, expressed as a percentage, %; Y is the seed germination rate, expressed as a percentage, %; M1 is the number of normal germinated grains within the germination potential days; M2 is the total number of normal germinated grains within the germination rate days; M is the total number of seeds in each group of samples.

[0067] The statistical results of germination potential and germination rate of grain seeds are shown in Table 1 below:

[0068] It can be seen from the above table that the average germination potential and the average germination rate of the grains treated by the methods provided in Examples 2 and 3 of the present application are significantly higher than those in the comparative example, that is, when the grains are not softened by the husk or lack a certain component in the composite abscisic acid release agent provided by the present application, the ideal germination effect cannot be achieved, which indicates that the various components of the composite abscisic acid release agent provided by the present application have obvious synergistic effects and are indispensable. It also indicates that the method for breaking the dormancy of millet provided by the present application has obvious advantages.

[0069] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0070] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for breaking millet dormancy for breeding experiments, characterized in that: The steps include: S1: immersing the pretreated ears of grain into an activation solution for activation treatment, wherein the activation solution contains a composite abscisic acid remover; S2: After the activated ears of millet are buried in a prefabricated matrix for a period of time, the dormancy of the millet is fully broken.

2. The method for breaking millet dormancy for breeding experiments as claimed in claim 1, characterized in that: The pretreatment process includes disinfection and softening of the ears of grain.

3. The method for breaking millet dormancy for breeding experiments as claimed in claim 2, characterized in that: The specific process of disinfecting the ears of grain is as follows: soaking the ears of grain in a sodium hypochlorite aqueous solution with a mass concentration of 1-3% for 15-20 minutes, then washing them with sterile water, and then taking them out and drying the surface moisture to obtain the disinfected ears of grain.

4. The method for breaking millet dormancy for breeding experiments as claimed in claim 2, characterized in that: The specific softening process of the grain ears is as follows: soak the grain ears in a 0.1-0.3 mol / L KOH solution for 10-15 minutes, then rinse them with sterile water, and then take them out and dry the surface moisture to obtain the softened grain ears.

5. The method for breaking millet dormancy for breeding experiments as claimed in claim 1, characterized in that: The composite abscisic acid remover is composed of oxalyl, edible torula yeast and gibberellin, wherein the mass ratio of oxalyl, edible torula yeast and gibberellin is 5-7:2-3:1-2.

6. The method for breaking millet dormancy for breeding experiments according to claim 1, characterized in that: The solvent of the activation solution is sterile water with an agar content of 2-5% by mass, and the ratio of the composite abscisic acid remover to the solvent is 1-3 g / L.

7. The method for breaking millet dormancy for breeding experiments according to claim 1, characterized in that: The activation treatment conditions are 12 to 48 hours at a water temperature of 25-40°C and a light intensity of 2000 to 3000 Lx.

8. The method for breaking millet dormancy for breeding experiments as claimed in claim 1, characterized in that: The peat soil is fully moistened with sterile water to ensure that the moistened peat soil does not drip when it is squeezed into a ball by hand to obtain a prefabricated matrix.

9. The method for breaking millet dormancy for breeding experiments according to claim 1, characterized in that: The ears of grain are buried in the pre-made substrate for 24 hours.

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