Milk fermented product filtrate as well as preparation method and application thereof
A technology for fermentation products and milk, applied in the field of milk fermentation product filtrate and its preparation, can solve problems such as difficult to overcome difficult problems, and achieve the effects of simplifying production steps, easy operation and ensuring stability
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Embodiment 1
[0035] [Example 1] The preparation method of milk fermentation product filtrate
[0036] Preparation of milk fermentation product filtrate: Weigh a certain amount of milk and water according to the ratio of 1:9 to obtain fermentation culture solution; after sterilizing at 95°C for 20 minutes, insert probiotics at an amount of 60g / ton, among which Lactobacillus plantarum and each 30g / ton of Bifidobacterium lactis to obtain a fermentation system. Ferment the fermentation system in a 37°C incubator for 48 hours to obtain a fermentation product; centrifuge the fermentation product at 6500r / min and a centrifugal radius of 9cm for 20min, discard the precipitate, collect the centrifuged supernatant, and filter it with a 0.22μm filter membrane After filtering, the supernatant was autoclaved at 108° C. for 20 minutes to obtain a sterilized fermentation filtrate, which was the milk fermentation product filtrate provided by the present invention.
Embodiment 2
[0037] [Example 2] Application of milk fermented product filtrate as cosmetics
[0038] 1. Safety testing of milk product fermentation filtrate
[0039] The human patch test is mainly used to detect the irritation of cosmetic final products or raw materials. The present invention conducts a human body closed patch test on the milk fermented product filtrate obtained in Example 1, aiming at evaluating its potential skin irritation.
[0040] 1. Test object
[0041] Choose 30 suitable volunteers, randomly selected in the age range of 18-60 years old.
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Abstract
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