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Method for preparing chitosan oligosaccharide by adopting cellulase spray-drying

A technology of cellulase and chitosan oligosaccharide, applied in fermentation and other directions, can solve the problems of high cost of physical degradation, large environmental pollution, and difficult reaction control, and achieves less environmental pollution, low production cost, and preparation time required. short effect

Inactive Publication Date: 2018-05-04
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the chemical degradation method needs to use a large amount of chemical reagents, which has great pollution to the environment, and the reaction is not easy to control
The cost of physical degradation is relatively high and is currently limited to the laboratory stage

Method used

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  • Method for preparing chitosan oligosaccharide by adopting cellulase spray-drying
  • Method for preparing chitosan oligosaccharide by adopting cellulase spray-drying
  • Method for preparing chitosan oligosaccharide by adopting cellulase spray-drying

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the preparation of chitosan oligosaccharide and the assay experiment of reducing sugar content

[0030] 1. Preparation of chitosan oligosaccharide by enzymatic hydrolysis of cellulase

[0031] Weigh 1.0g of chitosan and dissolve it in 0.2mol / L acetic acid-sodium acetate buffer solution with a pH value of 5.2 to prepare a chitosan solution with a mass concentration of 1.0%; weigh 0.4g of cellulase and dissolve it in a pH value of 5.2 0.2mol / L acetic acid-sodium acetate buffer solution to prepare a cellulase solution with a mass concentration of 0.4%; take 5mL chitosan solution, add 2mL cellulase solution, and react at 55°C for 4.5h to obtain the enzyme For solution, take 1mL of enzymatic solution and add 1mL of DNS reagent, after boiling in water for 5 minutes, add distilled water to make up to 10mL. Afterwards, use a centrifuge to centrifuge for 10 minutes, take the upper layer solution, use a UV spectrophotometer at a wavelength of 540nm, and use distill...

Embodiment 2

[0040] Embodiment 2, cellulase enzymatic hydrolysis preparation condition optimization experiment of chitosan oligosaccharide

[0041] 1. Chitosan concentration single factor experiment

[0042] Under the conditions of a fixed reaction temperature of 50°C and a reaction time of 4.5 h, 2 mL of cellulase solution with a pH value of 5.2 and 5 mL of chitosan solution were added, and the mass concentrations of chitosan were selected to be 0.5%, 0.8%, 1.0%, respectively. 1.2%, 1.6%, 2.0%, by measuring the reducing sugar content of the reaction as the evaluation index, the influence of the chitosan concentration on the reducing sugar content was investigated. The experimental results of the effect of chitosan concentration on reducing sugar content are as follows: figure 2 shown. Depend on figure 2 It can be seen that with the increase of chitosan concentration, the content of reducing sugar increases first and then decreases. When the concentration of chitosan is 1%, the conte...

Embodiment 3

[0056] Embodiment 3, spray drying process optimization experiment

[0057] 1. Selection of solid content

[0058] Under the condition of air inlet temperature of 180°C and feed rate of 700mL / h, the concentrated solution was concentrated to a solid concentration of 5%, 10%, 15%, 20%, and 25%, respectively, for single-factor comparative experiments. The dry chitosan oligosaccharide product moisture, recovery rate and spray-drying effect are used as evaluation indicators to investigate the experimental effects of different solid content and select the appropriate range of solid content. Comprehensive investigation and analysis of the influence of solid content on product moisture, recovery rate and spray drying effect, the experimental results are shown in Table 2.

[0059] Table 2: Effect of solid content on spray drying effect and product quality

[0060] Solid concentration (%)

[0061] It can be seen from Table 2 that the concentration of solids has an impact on p...

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Abstract

The invention belongs to the field of an oligosaccharide preparation method, and particularly relates to a method for preparing chitosan oligosaccharide by adopting cellulase spray-drying. The methodfor preparing the cellulase spray-drying comprises the following steps: dissolving a chitosan in an acetic acid-sodium acetate buffering solution, preparing a chitosan solution, adding a cellulase solution into the chitosan solution to perform enzymolysis, after the enzymolysis, dialyzing filtrate by adopting a dialysis method, concentrating a dialysis solution, and spray-drying. The method for preparing the chitosan oligosaccharide by adopting the cellulase spray-drying is mild in condition, simple, rapid, short in preparation time, low in price of cellulase, easy in obtaining the cellulase and low in production cost, and the prepared chitosan oligosaccharide is uniform in molecular weight distribution and uniform and consistent in color.

Description

technical field [0001] The invention belongs to the field of preparation methods of oligosaccharides, and in particular relates to a method for preparing chitosan oligosaccharides by spray drying with cellulase. Background technique [0002] Chitosan is a biodegradable and renewable natural amino polysaccharide that exists in large quantities in nature. It is composed of 2-glucosamine and 2-acetylglucosamine connected by β-1,4-glycosidic bonds. It is The only natural alkaline high polymer discovered so far is known as the sixth vital element of the human body besides carbohydrates, proteins, fats, minerals and vitamins. The degradation product of chitosan: chitosan oligosaccharide, not only has good water solubility, but also is easily absorbed by the human body. There are good prospects for expansion in agricultural product preservation and medicine. Oligochitosan has some common characteristics of sugars, and its activity is the highest when the degree of polymerization ...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12P19/14
CPCC12P19/14C12P19/26
Inventor 苏政权
Owner GUANGDONG PHARMA UNIV
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