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Anti-rheumatoid effective parts of Spiraea willow stems and branches, its preparation method and application

A technology of effective parts, Meadowsweet, applied in the field of medicine, can solve the problems of rare chemical components and pharmacological effects, and achieve the effect of inhibiting the pro-inflammatory cytokine tumor necrosis factor-α, high content of active ingredients, and clear ingredients

Inactive Publication Date: 2019-07-19
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rosaceae Spiraea genus Spiraea willowii is rich in resources. After searching the prior art literature, there are few research reports on its chemical constituents and pharmacological effects.

Method used

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  • Anti-rheumatoid effective parts of Spiraea willow stems and branches, its preparation method and application
  • Anti-rheumatoid effective parts of Spiraea willow stems and branches, its preparation method and application
  • Anti-rheumatoid effective parts of Spiraea willow stems and branches, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Screening of effective parts of Spiraea sibiricum stems and branches against rheumatoid arthritis

[0028] Preparation of Spiraea spiraea stem and branch extract and separated parts: Spiraea spiraea stems and branches 10 Kg, add 60% ethanol for reflux extraction three times, the amount of each time is 80L, 2 hours each time, filter, and combine the filtrate, Recover the ethanol under reduced pressure to obtain the extract. Add water to dissolve the extract. The amount is 5L. After chromatography on the macroporous adsorption resin column D101, the volume of the macroporous adsorption resin D101 is 5L. The eluent is eluted with water until the eluent is almost colorless. Deliquoring, concentrating under reduced pressure, and drying to obtain 475 g of water-eluted part of Spiraea stems and branches; eluting with 20% ethanol until the eluate is nearly colorless, collecting the eluate, concentrating under reduced pressure, and drying to obtain willow leaves 111 g of Spiraea st...

Embodiment 2

[0037] Determination of (+)-8'-hydroxypinoresin-8'- in the effective parts of the stems and branches of Spiraea serrata against rheumatoid arthritis by HPLC O -β-d-glucopyranoside, (7 R ,8 S )-Dihydrodehydrodiconiferin-9'- O -β-d-glucopyranoside, (+)-Cyclolignan-9- O- β-d-glucopyranoside and (+)-isolararchin-9- O -β-d-xylopyranoside content method

[0038] Chromatographic conditions and system suitability test

[0039] Use octadecylsilane-bonded silica gel as filler; use acetonitrile as mobile phase A and 0.1 phosphoric acid solution as mobile phase B, linear gradient elution: 0 min (A 10%, B 90%, volume ratio) → 10 min (A 10%, B 90%, volume ratio) → 30min (A 30%, B 70%, volume ratio) → 60 min (A 30%, B 70%); flow rate: 1.0 mL·min -1 ; The detection wavelength is 280nm. The number of theoretical plates press (+)-8'-hydroxypinoresin-8'- O -β-d-glucopyranoside calculation, not less than 6000.

[0040] Solution preparation

[0041] Preparation of reference solution

[0042] Weigh accurat...

Embodiment 3

[0048] Spiraea spiraea stems and branches 10 Kg (produced in Wangqi, Jilin), add 60% ethanol and reflux for three times, the amount of each time is 80L, 2 hours each time, filter, combine the filtrate, and recover the ethanol under reduced pressure to obtain the extract. Extract Add water to dissolve the substance. The amount is 5L. After chromatography on the macroporous adsorption resin column D101, the volume of the macroporous adsorption resin D101 is 5L. Elute with water until the eluent is nearly colorless. Discard the eluent and wash with 20% ethanol. Remove until the eluate is nearly colorless, discard the eluate, eluting with 50% ethanol until the eluate is nearly colorless, collect the eluate, concentrate under reduced pressure, and dry to get the stems and branches of Spiraea antirheumatoid The effective part of arthritis is 342 g. Measured by the method of Example 2, it contains 7.69% by weight of (+)-8'-hydroxypinoresin-8'- O -β-d-glucopyranoside, 12.17% by weight ...

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Abstract

The invention provides a spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and a preparation method and application thereof.According to the effective part, spiraea salicifolia stems and branches serve as raw materials, extract is obtained after 60% ethyl alcohol reflux extraction and ethyl alcohol pressure reduction recovery, the extract is purified with macroporous adsorption resin, and the total lignanoside effective part is obtained.The sum of weights of (+)-8'-hydroxy pinoresinol-8'-O-beta-d-glucopyranoside, (7R,8S)-dihydro dehydrogenation bis-coniferyl alcohol-9'-O-beta-d-glucopyranoside, (+)-lyonia ovalifolia tree ring lignans-9-O-beta-d-glucopyranoside and (+)-isolariciresinol-9-O-beta-d-pyran xyloside in the effective part ranges from 40% to 50%.The effective part is determined through the method that a modern separation means and pharmacological activity screening are combined, the ingredients of obtained effective part are clear, the content of active ingredients is high, it is shown through the in-vitro and in-vivo experiment results that the spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part inhibits generation of pro-inflammatory cytokine tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 to achieve the effect of treating rheumatoid arthritis.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to the effective parts of Spiraea spiraea stems and branches against rheumatoid arthritis and a preparation method thereof, and also relates to the effective parts of Spiraea spiraea stems and branches against rheumatoid arthritis in the preparation and treatment of rheumatoid arthritis. Use in arthritis products. Background technique [0002] Rheumatoid arthritis is a common autoimmune disease characterized by chronic inflammatory lesions of joint tissues. The main pathological changes are joint synovial cell infiltration, synovial pannus formation, and erosion of cartilage and bone tissue. The purpose of rheumatoid arthritis treatment is to prevent the progression of the disease, that is, to prevent the joint inflammation and destruction process, and to prevent loss of function. Although there are many treatment measures, drug treatment is still the basis. Current treatment drugs include non-s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61K31/7048A61K31/704A61P29/00A61P19/02
CPCA61K31/704A61K31/7048A61K36/73A61K2236/333A61K2236/39A61K2236/55A61K2300/00
Inventor 王威董方言刘小红韩立春师海波刘洋
Owner QINGDAO UNIV
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