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Spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and preparation method and application thereof

A technology of effective parts and spiraea, applied in the field of medicine, can solve the problems of rare chemical components and pharmacological effects, and achieve the effects of convenient clinical use, high content of active ingredients and low energy consumption

Inactive Publication Date: 2016-07-27
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rosaceae Spiraea genus Spiraea willowii is rich in resources. After searching the prior art literature, there are few research reports on its chemical constituents and pharmacological effects.

Method used

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  • Spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and preparation method and application thereof
  • Spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and preparation method and application thereof
  • Spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Screening of Effective Parts from Stems and Branches of Spiraea lanceolata for Anti-rheumatoid Arthritis

[0028] Preparation of Spiraea willow stems and branches extract and isolated parts: 10Kg of Spiraea willows stems and branches, add 60% ethanol to reflux and extract three times, each dosage is 80L, each time for 2 hours, filter, the filtrates are combined, reduce Recover the ethanol under pressure to obtain the extract, add water to dissolve the extract, the dosage is 5L, go through the macroporous adsorption resin column D101 chromatography, fill the macroporous adsorption resin D101 with a volume of 5L, elute with water until the eluate is nearly colorless, collect the eluted liquid, concentrated under reduced pressure, and dried to obtain 475 g of water-eluted parts of Spiraea lanceolata stems and branches; eluted with 20% ethanol until the eluate was nearly colorless, collected the eluent, concentrated under reduced pressure, and dried to obtain Spiraea spiraea...

Embodiment 2

[0037] Determination of (+)-8'-hydroxypinoresin-8'-O-β-d-glucopyranoside, (7R,8S)- Dihydrodehydrobiconiferyl-9'-O-β-d-glucopyranoside, (+)-peariginadin-9-O-β-d-glucopyranoside and (+) - Method for the content of isolacitin-9-O-β-d-xylopyranoside

[0038] Chromatographic conditions and system suitability test

[0039] Octadecylsilane-bonded silica gel is used as filler; acetonitrile is used as mobile phase A, 0.1 phosphoric acid solution is used as mobile phase B, linear gradient elution: 0min (A10%, B90%, volume ratio) → 10min (A10% , B90%, volume ratio) → 30min (A30%, B70%, volume ratio) → 60min (A30%, B70%); flow rate: 1.0mL min -1; The detection wavelength is 280nm. The number of theoretical plates is calculated based on (+)-8'-hydroxypinoresin-8'-O-β-d-glucopyranoside, not less than 6000.

[0040] Solution preparation

[0041] Preparation of reference solution

[0042] Precisely weigh (+)-8'-hydroxypinetin-8'-O-β-d-glucopyranoside, (7R,8S)-dihydrodehydrobiconiferyl a...

Embodiment 3

[0048] Spiraea willow stems and branches 10Kg (produced in Wangqi, Jilin), add 60% ethanol to reflux and extract three times, each dosage is 80L, each time for 2 hours, filter, combine the filtrates, recover the ethanol under reduced pressure to obtain the extract, extract Add water to dissolve, the dosage is 5L, go through macroporous adsorption resin column D101 chromatography, fill the volume of macroporous adsorption resin D101 to 5L, elute with water until the eluate is nearly colorless, discard the eluent, and elute with 20% ethanol When the eluent is nearly colorless, discard the eluent, elute with 50% ethanol until the eluent is almost colorless, collect the eluate, concentrate under reduced pressure, and dry to obtain the rheumatoid resistance of Spiraea willow stems and branches Arthritis effective fraction 342g. Measured by the method of Example 2, containing 7.69% by weight of (+)-8'-hydroxypinoresin-8'-O-β-d-glucopyranoside, 12.17% by weight of (7R,8S)-dihydro De...

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Abstract

The invention provides a spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part and a preparation method and application thereof.According to the effective part, spiraea salicifolia stems and branches serve as raw materials, extract is obtained after 60% ethyl alcohol reflux extraction and ethyl alcohol pressure reduction recovery, the extract is purified with macroporous adsorption resin, and the total lignanoside effective part is obtained.The sum of weights of (+)-8'-hydroxy pinoresinol-8'-O-beta-d-glucopyranoside, (7R,8S)-dihydro dehydrogenation bis-coniferyl alcohol-9'-O-beta-d-glucopyranoside, (+)-lyonia ovalifolia tree ring lignans-9-O-beta-d-glucopyranoside and (+)-isolariciresinol-9-O-beta-d-pyran xyloside in the effective part ranges from 40% to 50%.The effective part is determined through the method that a modern separation means and pharmacological activity screening are combined, the ingredients of obtained effective part are clear, the content of active ingredients is high, it is shown through the in-vitro and in-vivo experiment results that the spiraea salicifolia stem and branch anti-rheumatoid-arthritis effective part inhibits generation of pro-inflammatory cytokine tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 to achieve the effect of treating rheumatoid arthritis.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the anti-rheumatoid arthritis effective parts of Spiraea lanceolata stems and a preparation method thereof, and also relates to the anti-rheumatoid arthritis effective parts of Spiraea willow stems and branches in the preparation and treatment of rheumatoid arthritis. Uses in Arthritis Products. Background technique [0002] Rheumatoid arthritis is a common autoimmune disease characterized by chronic inflammatory lesions of joint tissues. The main pathological changes are the infiltration of synovial cells, the formation of synovial pannus, and the erosion of cartilage and bone tissue. The goal of rheumatoid arthritis treatment is to arrest the progression of the disease, that is, to stop the process of joint inflammation and destruction, and to prevent loss of function. Although there are many treatment measures, drug treatment is still the basis. Current treatments include non-steroida...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K31/7048A61K31/704A61P29/00A61P19/02
CPCA61K31/704A61K31/7048A61K36/73A61K2236/333A61K2236/39A61K2236/55A61K2300/00
Inventor 王威董方言刘小红韩立春师海波刘洋
Owner QINGDAO UNIV
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