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Synchronous detection method of coniferyl alcohol and pinoresinol

A simultaneous detection and pinoresinol technology, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to detect coniferyl alcohol and pinoresinol content, and inability to separate coniferyl alcohol and pinoresinol.

Active Publication Date: 2019-05-21
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because the structures of coniferyl alcohol and pinoresinol are relatively similar, their physical and chemical properties are also relatively similar. Therefore, it is currently impossible to separate coniferyl alcohol and pinoresinol with a simple and effective method, and it is also impossible to detect the content of coniferyl alcohol and pinoresinol.

Method used

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  • Synchronous detection method of coniferyl alcohol and pinoresinol
  • Synchronous detection method of coniferyl alcohol and pinoresinol
  • Synchronous detection method of coniferyl alcohol and pinoresinol

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preparation example Construction

[0077] In the present invention, the preparation method of the standard solution of coniferyl alcohol comprises: mixing coniferyl alcohol with 100% chromatographic grade methanol to obtain the standard solution of coniferyl alcohol; The volume ratio is (0.5-2) mg: 1 mL.

[0078] In the present invention, the preparation method of the standard solution of pinoresinol comprises: mixing pinoresinol with 100% chromatographic grade methanol to obtain the standard solution of pinoresinol; The volume ratio is (0.5-2) mg: 1 mL.

[0079] In the present invention, the concentrations of coniferyl alcohol and pinoresinol in the mixed standard solution in step ② are 400-600 μg / mL.

[0080] In the present invention, the chromatographic conditions for testing the absorbance in the step ③ are the same as the chromatographic conditions for testing the absorbance in the sample to be tested, and will not be repeated here.

Embodiment 1

[0083] A kind of synchronous detection method of coniferyl alcohol and pinoresinol of the present embodiment, comprises the steps:

[0084] (1) Drawing of standard curve

[0085] Weigh 5 mg of the standard products of coniferyl alcohol and pinoresinol, dissolve them in 5 mL of 100% chromatographic grade methanol solution; Standard solution, and measure the absorbance of coniferyl alcohol and pinoresinol under corresponding concentration, draw standard curve, obtain the standard curve of coniferyl alcohol and pinetisol standard curve respectively.

[0086] The standard curve of the measured coniferyl alcohol is y 1 =893.37x 1 -3.326, as in figure 1 shown, where x 1 is the concentration of coniferyl alcohol, in mg / mL, y 1 is the absorbance of coniferyl alcohol, the correlation coefficient R 2 =09988, the linear range is: 0~2.5mg / mL;

[0087] The standard curve of pinoresinol is y 2 =1471.13x 2 +13.817, such as figure 2 shown, where x 2 is the concentration of pinores...

Embodiment 2

[0101] A kind of synchronous detection method of coniferyl alcohol and pinoresinol of the present embodiment, comprises the steps:

[0102] (1) Drawing of standard curve

[0103] With embodiment 1;

[0104] (2) Preparation of the sample solution to be tested

[0105] The Dangshansu pear young pear fruit was peeled and cored, cut into quarters, weighed 2g of the sample to be tested and mixed with 20mL of 90% ethanol aqueous solution, ultrasonically extracted at 60°C for 30min, and then centrifuged at a speed of Centrifuge at 5000r / min for 10min; take the centrifuged supernatant and dry it with nitrogen, then add 2mL of 100% chromatographic grade methanol to redissolve; filter the redissolved solution with an organic filter membrane with a pore size of 0.20μm, and obtain the filtrate As the test solution.

[0106] (3) Determination of samples to be tested

[0107] Using high performance liquid chromatography, the sample solution to be tested is tested under the following chr...

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Abstract

The invention relates to the field of substance separation detection and provides a synchronous detection method of coniferyl alcohol and pinoresinol. Absorbance values of coniferyl alcohol and pinoresinol in a to-be-detected sample are measured by using high performance liquid chromatography, wherein the detection wavelength is 280 nm and the high-performance liquid chromatography employs gradient elution and the eluent in the gradient elution includes methanol and water; according to a standard curve of coniferyl alcohol and the absorbance of the coniferyl alcohol in the coniferyl alcohol, the concentration of the coniferyl alcohol in the to-be-detected sample is obtained; and on the basis of the standard curve of pinoresinol and the absorbance of pinoresinol in the to-be-detected sample, the concentration of the pinoresinol in the to-be-detected sample is obtained. Therefore, the coniferyl alcohol and pinoresinol in the to-be-detected sample can be separated effectively; and the corresponding concentrations of the coniferyl alcohol and pinoresinol can be detected respectively.

Description

technical field [0001] The invention relates to the field of substance separation and detection, in particular to a method for synchronous detection of coniferyl alcohol and pinoresinol. Background technique [0002] Coniferyl alcohol and pinoresinol are key intermediate metabolites in the process of pear secondary metabolism to form lignin, and they are widely distributed in plants. Among them, pinoresinol is formed by the polymerization of two molecules of coniferyl alcohol. This dimer, also known as lignan, can couple more lignin monomers to form lignin polymers. By studying the content changes of coniferyl alcohol and pinoresinol, it is beneficial to better study the metabolic pathway of pears. But a kind of method that can separate coniferyl alcohol and pinoresinol in pear and other plants, and detect the content of coniferyl alcohol and pinoresinol in pears or other plants has not been found yet. [0003] At present, no matter in the field of chemistry or in the fiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 蔡永萍李国辉程曦张晴晴王涵孙燕铭江桃山金青林毅
Owner ANHUI AGRICULTURAL UNIVERSITY
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