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A kind of synchronous detection method of coniferyl alcohol and pinoresinol

A technology for synchronous detection and pinoresinol, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as the inability to separate coniferyl alcohol and pinoresinol, and the inability to detect the content of coniferyl alcohol and pinoresinol

Active Publication Date: 2021-08-31
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because the structures of coniferyl alcohol and pinoresinol are relatively similar, their physical and chemical properties are also relatively similar. Therefore, it is currently impossible to separate coniferyl alcohol and pinoresinol with a simple and effective method, and it is also impossible to detect the content of coniferyl alcohol and pinoresinol.

Method used

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  • A kind of synchronous detection method of coniferyl alcohol and pinoresinol
  • A kind of synchronous detection method of coniferyl alcohol and pinoresinol
  • A kind of synchronous detection method of coniferyl alcohol and pinoresinol

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preparation example Construction

[0077] In the present invention, a method of preparing the standard solution coniferyl alcohol comprising: coniferyl alcohol was mixed with 100% HPLC grade methanol to give coniferyl alcohol standard solution; the mass of coniferyl alcohol and 100% of chromatography grade methanol volume ratio of (0.5 ~ 2) mg: 1mL.

[0078] In the present invention, a method of preparing the standard solution pinoresinol comprises: mixing a rosin with an alcohol of 100% HPLC grade methanol to obtain standard solutions of pinoresinol; mass of the pinoresinol and 100% of chromatography grade methanol volume ratio of (0.5 ~ 2) mg: 1mL.

[0079] In the present invention, the step ② mixed standard solution and the concentrations of coniferyl alcohol rosin alcohol is 400 ~ 600μg / mL.

[0080] In the present invention, the step ③ chromatographic conditions tested and absorbance chromatographic conditions tested in the test sample absorbance same, are not repeated here.

Embodiment 1

[0083] A method for detecting synchronization coniferyl alcohol pinoresinol embodiment according to the present embodiment, comprising the steps of:

[0084] Draw (1) Standard curve

[0085] Weigh 5mg alcohol coniferyl alcohol rosin standards, were dissolved in 5mL100% HPLC grade methanol solution; then, it was diluted to 125μg / mL, 250μg / mL, 500μg / mL, 1000μg / mL, 2000μg / mL of standard solution, and the absorbance was measured at pinoresinol coniferyl alcohol corresponding to the concentration of the standard curve, respectively, to obtain the standard curve of coniferyl alcohol and terpineol standard curve.

[0086] From a standard curve of coniferyl alcohol is y 1 = 893.37x 1 -3.326, such as figure 1 , In which x 1 Coniferyl alcohol concentration, in units of mg / mL, y 1 Absorbance coniferyl alcohol, correlation coefficient R 2 = 09 988, the linear range: 0 ~ 2.5mg / mL;

[0087] Standard curve is y pinoresinol 2 = 1471.13x 2 +13.817, such as figure 2 , In which x 2 Pin...

Embodiment 2

[0101] The synchronous detection method of a pine-proximal alcohol and pneryl alcohol, including the following steps:

[0102] (1) Drawing of standard curves

[0103] In the same embodiment 1;

[0104] (2) Preparation of sample solution to be tested

[0105] The peeled extrapolate of the osispar pear pear is removed, and the 2-point method is cut, and the 2G to be tested is mixed with a 20 ml mass concentration of 90% ethanol, and the ultrasound is extracted at 60 ° C for 30 min, then at the centrifuge speed. Centrifuge was centrifuged under 5000 r / min; mixed with nitrogen after centrifugation, then blended with nitrogen, then add 2 ml of 100% chromatographic methanol; filtered with an organic filtration of the solution of 0.20 μm, the resulting filtrate Examples of the test solution.

[0106] (3) Determination of sample to be tested

[0107] With high performance liquid chromatography, the sample solution to be tested was tested under the following chromatographic conditions to...

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Abstract

The invention relates to the field of separation and detection of substances, and provides a method for synchronous detection of coniferyl alcohol and pinoresinol. First, high performance liquid chromatography is used to measure the absorbance of coniferyl alcohol and pinoresinol in a sample to be tested, wherein the detection wavelength is 280nm; The high-performance liquid chromatography adopts gradient elution, and the eluent in the gradient elution includes methanol and water; then according to the standard curve of coniferyl alcohol and the absorbance of coniferyl alcohol in the test sample, the coniferyl alcohol in the test sample is obtained. The concentration of alcohol; According to the standard curve of pinoresinol and the absorbance of pinoresinol in the test sample, obtain the concentration of pinoresinol in the test sample. The method provided by the invention can effectively separate coniferyl alcohol and pinoresinol in the sample to be tested, and detect the corresponding concentrations of coniferyl alcohol and pinoresinol respectively.

Description

Technical field [0001] The present invention relates to material separation and detection, and more particularly relates to a method for detecting synchronization rosin alcohol coniferyl alcohol. Background technique [0002] Coniferyl alcohol and terpineol Pear is a key intermediate metabolites secondary metabolites formed during lignin, they are widely distributed in plants. Wherein, pinoresinol by polymerization of coniferyl alcohol from two molecules, such dimers, also called lignans, lignin polymer may be formed by coupling more lignin monomer. By studying changes in the content of coniferyl alcohol and turpentine alcohol, metabolic pathways better research pear favor. However, today has not been found capable of separating pear and other plants rosin alcohol coniferyl alcohol, coniferyl alcohol and terpineol method of detecting the content of pear or other plants. [0003] At present, both in the field of chemistry or biological field research on alcohol coniferyl alcohol a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 蔡永萍李国辉程曦张晴晴王涵孙燕铭江桃山金青林毅
Owner ANHUI AGRICULTURAL UNIVERSITY
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