Antioxidant extract of wedelia chrysanthemum flower and its preparation method and application

An extract and antioxidant technology, applied in the application, antidote, drug combination and other directions, can solve the problems of separation and identification of chemical components of the antioxidative effective parts of the South American weed chrysanthemum, and achieve low cost and excellent antioxidant activity. , the effect of clear chemical composition

Active Publication Date: 2021-05-07
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no patents related to the anti-oxidation application of Wedelia chinensis, and there is no report on the separation and identification of effective antioxidant parts and further chemical components of Wedelia chinensis.

Method used

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  • Antioxidant extract of wedelia chrysanthemum flower and its preparation method and application
  • Antioxidant extract of wedelia chrysanthemum flower and its preparation method and application
  • Antioxidant extract of wedelia chrysanthemum flower and its preparation method and application

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Experimental program
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Effect test

preparation example Construction

[0026] The preparation method step of South American Wedelia chrysanthemum extract is as follows:

[0027] 1) Add 85-95% ethanol or methanol aqueous solution to the pulverized Wedelia chrysanthemum medicinal material (passed through a 20-80 mesh sieve), soak at room temperature, heat or ultrasonically extract, the extraction time is 60 minutes to 7 days, and the solid-liquid ratio 1:1~1:10;

[0028] 2) Filtrate the extract of Wedelia chrysanthemum medicinal materials, recover under reduced pressure to obtain the total extract, add water to suspend, sequentially extract with petroleum ether and ethyl acetate, recover under reduced pressure to obtain the petroleum ether part, ethyl acetate part and water-soluble part respectively ;

[0029] 3) Among them, the ethyl acetate part is chromatographed on a silica gel column, first eluted with chloroform:ethyl acetate (1:0-10:1) to remove petroleum ether extraction residues and small polar oil components, and then eluted with acetone...

Embodiment 2

[0036] Example 2 Study on Antioxidant Activity of Wedelia chrysanthemum Flower Extract

[0037] 1. Wedelia chrysanthemum flower extract scavenging DPPH free radical activity

[0038] 1.1 Experimental method: 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) was prepared into a 65 μM absolute ethanol solution. Precisely weigh the test samples and dissolve them in absolute ethanol for later use.

[0039] Pipette 50 μL of the test sample and add it to 3 mL of 65 μM DPPH solution, and use an equal amount of absolute ethanol for the blank control, mix well, place it at room temperature for 30 minutes, and then place it in a cuvette to measure the absorbance at 517 nm of the mixed solution of the sample and DPPH. Each absorbance was measured 3 times in parallel, and the average value was taken to calculate the clearance rate of the series of concentrations to obtain the IC 50 value. The clearance rate (Y) is calculated according to the following formula:

[0040] Y=[l-(Ai-Aj) / Ao]×10...

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Abstract

The invention discloses an antioxidant extract of wedelia chrysanthemum flowers, a preparation method and application thereof. The extract is extracted by adding a solvent to the dry powder of Wedelia chrysanthemum; the extract is filtered and concentrated under reduced pressure to obtain the total extract, which is sequentially extracted with petroleum ether and ethyl acetate, and recovered under reduced pressure to obtain petroleum ether and ethyl acetate respectively. The ester part and the water-soluble part, the obtained ethyl acetate part and the water-soluble part have excellent scavenging DPPH free radicals and anti-fat oxidation activity, wherein the ethyl acetate part contains 9 flavonoids and 4 phenylpropanoids. The yield of the extract prepared by the invention is high, the process flow is simple, and it is suitable for large-scale preparation. The extract has excellent antioxidant activity and can be used as an active component in antioxidant drugs and food preservation.

Description

technical field [0001] The invention belongs to the field of natural plant extraction, and in particular relates to an anti-oxidation extract of Wedelia chrysanthemum flower, its preparation method and application. Background technique [0002] South American Wedelia (Wedelia trilobata), also known as three-lobed (leaf) Wedelia, is a perennial herbaceous plant of the genus Wedelia in the family Compositae. It is native to central and southern tropical America. The leaves or aerial parts of the plant are used in traditional medicine in the Caribbean and Central America to treat back pain, muscle spasms, rheumatism, stubborn wounds, ulcers, swelling, and joint pain. It was first introduced to Hong Kong in the 1870s to replace the traditional Chinese medicine Wedelia, mainly used to treat diphtheria, whooping cough, dysentery, hemorrhoids, and bruises. Nowadays, on the roadsides, fields, ditches or wet grasslands widely distributed in Guangdong, Guangxi, Fujian and Hainan, etc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/83C07D311/40C07D311/32C07C65/21C07C67/48C07C57/04A61K36/28A61P39/06A23L3/3472
Inventor 陈文豪惠阳陈光英宋小平武晓雪周秀悄
Owner HAINAN NORMAL UNIV
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