Preparation method of Psychotria hainanensis stem extract

A technology of stem extract and extract, which is applied in the field of extraction and separation of active ingredients of natural plants, to achieve the effect of clear chemical composition, low cost, and good antibacterial activity

Active Publication Date: 2019-10-01
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few researches on the basis of medicinal substances and pharmacological activities related to Hainan Jiujie.

Method used

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  • Preparation method of Psychotria hainanensis stem extract
  • Preparation method of Psychotria hainanensis stem extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 The preparation of the effective part of Hainan nine-section stem

[0025] Hainan nine-section stem (10Kg) is dried and crushed in the shade, soaked in petroleum ether (60-90°C) at room temperature for 3 times, 7 days / time, combined extracts, concentrated under reduced pressure to obtain petroleum ether-dissolved part extract (78g); After evaporating the residual petroleum ether, soak it in ethyl acetate at room temperature for 3 times, 7 days / time, combine the extracts, concentrate under reduced pressure to obtain the part of the extract dissolved in ethyl acetate (150g); , using ethanol aqueous solution (50-70%) to soak at room temperature for 3 times, 7 days / time, combine the extracts, concentrate under reduced pressure to obtain ethanol-dissolved partial extracts (180g).

[0026] The petroleum ether dissolved part is chromatographed on a silica gel column, and petroleum ether is used to elute to remove small polar mixtures such as long-chain grease wax ...

Embodiment 2

[0028] Example 2 Study on the Antibacterial Activity of Different Solvent Dissolved Parts and Monomer Compounds of Hainan Nine-section Stem

[0029] 1. Experimental method: 5 cultured strains of human pathogenic bacteria (tetrazyme, bacillus cereus, bacillus subtilis, staphylococcus albus, staphylococcus aureus) were mixed with 1:500-1:1000 Dilute the drug in DMSO to make 1 mg / mL for use, add 10 μL of the stock solution to each well of the first row of the 96-well plate, and put ciprofloxacin in a separate well as a positive control; then take 190 μL of the diluted strain solution , add to the first row of 96-well plate, mix well, then take 100 μL from the first row and add it to the second row, and so on until the 100 μL diluted bacterial solution from the last row is taken out and discarded; finally add 100 μL diluted bacterial solution to each well, The final volume of the well is 200 μL; incubate in a 37°C incubator for 8-10 hours, and observe the turbidity in the well wit...

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Abstract

The invention discloses a preparation method of Psychotria hainanensis stem extract, and the method comprises the following steps: adding a petroleum ether solvent into Psychotria hainanensis stem drypowder for extraction; filtering and concentrating an extractive solution under reduced pressure to obtain petroleum ether dissolved extract, subjecting the petroleum ether dissolved extract to silica gel column chromatography, eluting with pure petroleum ether as a solvent to remove a long-chain oil wax component, eluting and flushing the column with ethyl acetate, and vacuum concentrating eluate to obtain petroleum ether extracted essence with wide antibacterial activity; and repeatedly carrying out column chromatography on the petroleum ether extracted essence to obtain seven triterpenoids, one sterol compound and two flavonoids. The Psychotria hainanensis stem extract prepared by the method has the advantages of high yield, simple process flow and low cost, is suitable for large-scalepreparation, has good antibacterial activity, and can be applied to auxiliary antibacterial and anti-inflammatory drugs.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective components of natural plants, and relates to a preparation method of an extract of hainan nine-section stem, in particular to a kind of hainan nine-section stem extract which has good antibacterial activity and can be widely used in anti-inflammatory drugs method of preparation. Background technique [0002] Rubiaceae (Rubiaceae) genus Psychotria has about 800-1500 species in the world, distributed in tropical and subtropical regions, especially in America. This genus of plants is a traditional medicinal material in China, mainly used to treat fractures, snake bites and bruises. It has a long history of medicinal use. As a traditional therapeutic drug for clearing away heat, detoxifying, reducing swelling and removing toxins, the plants of the genus Ninesections have great development potential in the research of their antibacterial activity. [0003] Although plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07J71/00C07D311/30C07D311/40A61P31/04
CPCA61P31/04C07D311/30C07D311/40C07J63/008C07J71/0005
Inventor 陈文豪惠阳曹坚陈光英宋小平
Owner HAINAN NORMAL UNIV
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