A kind of preparation method of Hainan nine knot stem extract

A stem extract and extract technology are applied in the field of extraction and separation of natural plant active ingredients to achieve the effects of clear chemical composition, high yield and simple process flow

Active Publication Date: 2021-10-01
HAINAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few researches on the basis of medicinal substances and pharmacological activities related to Hainan Jiujie.

Method used

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  • A kind of preparation method of Hainan nine knot stem extract
  • A kind of preparation method of Hainan nine knot stem extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 The preparation of the effective part of Hainan nine-section stem

[0025] Hainan nine-section stem (10Kg) is dried and crushed in the shade, soaked in petroleum ether (60-90°C) at room temperature for 3 times, 7 days / time, combined extracts, concentrated under reduced pressure to obtain petroleum ether-dissolved part extract (78g); After evaporating the residual petroleum ether, soak it in ethyl acetate at room temperature for 3 times, 7 days / time, combine the extracts, concentrate under reduced pressure to obtain the part of the extract dissolved in ethyl acetate (150g); , using ethanol aqueous solution (50-70%) to soak at room temperature for 3 times, 7 days / time, combine the extracts, concentrate under reduced pressure to obtain ethanol-dissolved partial extracts (180g).

[0026] The petroleum ether dissolved part is chromatographed on a silica gel column, and petroleum ether is used to elute to remove small polar mixtures such as long-chain grease wax ...

Embodiment 2

[0028] Example 2 Study on the Antibacterial Activity of Different Solvent Dissolved Parts and Monomer Compounds of Hainan Nine-section Stem

[0029] 1. Experimental method: 5 cultured strains of human pathogenic bacteria (Tetreccus, Bacillus cereus, Bacillus subtilis, Staphylococcus albus, Staphylococcus aureus) were mixed with culture medium at a ratio of 1:500-1:1000. Dilute the drug in DMSO to make 1 mg / mL for use, add 10 μL of the stock solution to each well of the first row of the 96-well plate, and put ciprofloxacin in a separate well as a positive control; then take 190 μL of the diluted strain solution , add to the first row of 96-well plate, mix well, then take 100 μL from the first row and add it to the second row, and so on until the 100 μL diluted bacterial solution from the last row is taken out and discarded; finally add 100 μL diluted bacterial solution to each well, The final volume of the well is 200 μL; incubate in a 37°C incubator for 8-10 hours, and observe...

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Abstract

The invention discloses a preparation method of Hainan nine-section stem extract, which is to add petroleum ether solvent to the dry powder of Hainan nine-section stem to extract; The extract was chromatographed on a silica gel column, using pure petroleum ether as a solvent to elute the long-chain oil and wax components in the extract, and then eluted with ethyl acetate to wash the column, and the effluent was concentrated in vacuum to obtain a compound with a wide range of antibacterial activities. Petroleum ether extract essence; the petroleum ether extract essence was subjected to repeated column chromatography to obtain 7 triterpenoids, 1 sterol compound and 2 flavonoids. The extract prepared by the invention has high yield, simple process flow and low cost, and is suitable for large-scale preparation. The extract has good antibacterial activity and can be used in auxiliary antibacterial and anti-inflammatory drugs.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective components of natural plants, and relates to a preparation method of an extract of hainan nine-section stem, in particular to a kind of hainan nine-section stem extract which has good antibacterial activity and can be widely used in anti-inflammatory drugs method of preparation. Background technique [0002] Rubiaceae (Rubiaceae) genus Psychotria has about 800-1500 species in the world, distributed in tropical and subtropical regions, especially in America. This genus of plants is a traditional medicinal material in China, mainly used to treat fractures, snake bites and bruises. It has a long history of medicinal use. As a traditional therapeutic drug for clearing away heat, detoxifying, reducing swelling and removing toxins, the plants of the genus Ninesections have great development potential in the research of their antibacterial activity. [0003] Although plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07J71/00C07D311/30C07D311/40A61P31/04
CPCA61P31/04C07D311/30C07D311/40C07J63/008C07J71/0005
Inventor 陈文豪惠阳曹坚陈光英宋小平
Owner HAINAN NORMAL UNIV
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