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Method for preparing oligomeric procyanidins from lychee extract

A technology of litchi extract and proanthocyanidin, which is applied in the direction of organic chemistry, can solve the problems of undiscovered litchi extract component extraction, undiscovered research reports on the hydrolysis of proanthocyanidin polymers with organic acids, etc., to achieve full utilization and increase the effect of content

Active Publication Date: 2017-03-08
合浦果香园食品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the relevant technology of litchi extract only involves the extraction method of litchi extract, and no extraction of specific components in litchi extract has been found.
And so far, only relevant researches on the hydrolysis of proanthocyanidin high polymers with inorganic acids have been found, and no relevant research reports on the hydrolysis of proanthocyanidin high polymers with organic acids have been found.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Extraction: Clean the collected fresh lychee skin, lychee core, lychee physiological fruit drop, and lychee leaves, then break them, and extract according to the weight ratio of water and raw materials at 3:1, start timing when the leaching reaches 80°C, and keep at 80- 90°C for 3 hours, extract once to obtain the extract;

[0021] 2. Centrifugation: Centrifuge the extract in a low-speed centrifuge at a speed of 4000r / min for 10 minutes to obtain a centrifugal extract;

[0022] 3. Concentration: Concentrate the centrifugal extract in vacuum to 40 0 BX, wherein the concentrated temperature is controlled at 55-60°C and the vacuum degree is controlled at 0.09-0.1MPa to obtain 40 0 BX centrifugal extraction concentrate;

[0023] 4. Separation and purification: use AB-8 macroporous resin, according to the ratio of 1:1.5 between the amount of resin and the centrifugal extraction concentrate, feed at a rate of 2BV / h; sequentially use pure water and 5% ethanol at a rate of...

Embodiment 2

[0028] 1. Extraction: Clean the collected fresh lychee peels without breaking them, extract according to the weight ratio of water and raw materials 3:1, start timing when the extraction reaches 80°C, and keep at 80-90°C for 2 hours, extract twice , to obtain the extract;

[0029] 2. Centrifugation: put the extract in a low-speed centrifuge at a speed of 3000r / min, and centrifuge for 5 minutes;

[0030] 3. Concentration: Concentrate the extract in vacuum to 20 0 BX, wherein the concentrated temperature is controlled at 50-60°C and the vacuum degree is controlled at 0.09-0.1MPa to obtain 20 0 Centrifugal extract concentrate of BX;

[0031] 4. Tartaric acid hydrolysis concentrate: hydrolyze the centrifuged extraction concentrate with tartaric acid, wherein the hydrolysis system is: add 23ml of 5% tartaric acid hydrolyzate to 2ml of the extract concentrate, and bathe in 40°C for 45min to obtain the tartaric acid hydrolysis concentrate;

[0032] 5. Separation and purification: ...

Embodiment 3

[0036] 1. Extraction: Clean the collected fresh lychee leaves without breaking them and bundle them. Extract according to the weight ratio of water and raw materials at 1:1. Start timing when the extraction reaches 80°C, and keep at 80-90°C for 1 hour to extract 3 times to obtain the extract;

[0037] 2. Centrifugation: Centrifuge the extract in a low-speed centrifuge at a speed of 5000r / min for 30 minutes to obtain a centrifugal extract;

[0038] 3. Concentration: Concentrate the centrifugal extract in vacuum to 60 0 BX, wherein the concentrated temperature is controlled at 55-65°C and the vacuum degree is controlled at 0.09-0.1MPa to obtain 20 0 BX centrifugal extraction concentrate;

[0039] 4. Separation and purification: use LX-36 macroporous resin, according to the ratio of resin amount and centrifugal extraction concentrate 1:2, feed at a rate of 1BV / h; sequentially use pure water and 30% ethanol at a rate of 2BV / h Impurities are eluted until the collected liquid is cl...

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Abstract

The invention discloses a method for preparing oligomeric procyanidins from lychee extract. Lychee contains a lot of procyanidins, and the procyanidins are provided with strong antioxidant activity and free radical scavenging activity. No extraction method for oligomeric procyanidins has been found until now. The method is characterized in that the procyanidins in the lychee extract are specifically hydrolyzed through a method of a first extraction, a second centrifugation, a third enrichment, a fourth separation and purification, a fifth enrichment by polyphenol eluents, a sixth drying and a seventh hydrolysis by organic acids, reducing the polymerization of the procyanidins, greatly increasing the content of monomeric and oligomeric procyanidins in the lychee extract, and making the natural resources of the procyanidins in the lychee extract more fully utilized. After the hydrolysis of the organic acids, polyphenol content in the dry powder of the extract is more than 70%, the average degree of polymerization of the procyanidins is less than 2.0, the content of the procyanidin monomer epicatechin is more than 6.5%, the content of the procyanidin oligomer A1 is more than 5.0%, and the content of the procyanidin oligomer A2 is more than 4.0%.

Description

technical field [0001] The invention relates to a method for preparing oligomer-containing proanthocyanidins from litchi extract, which belongs to the technical field of food. Background technique [0002] Litchi is the fruit of Sapindaceae lychee. The flesh is sweet and juicy, delicious, delicious and has high nutritional value. Litchi contains a large amount of proanthocyanidins (PC), which is a general term for a large class of polyphenolic compounds that widely exist in the plant kingdom. Procyanidins in nature are composed of different amounts of catechin and epicatechin. of. According to the degree of polymerization, 2-4 polymers are usually called oligomers (OPC), and 5-mers and above are called high polymers (PPC). Among them, the simplest proanthocyanidins are catechin, or epicatechin, or dimers formed by catechin and epicatechin, in addition to trimers, tetramers and even decamers. Because of the different connection methods of the monomers, it can be divided in...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 黄辛满兴宁进辉戚世梅张健英
Owner 合浦果香园食品有限公司
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