Method for separating and purifying kinds of flavonoid constituents from meconopsis integrifolia flowers at same time
A technique for simultaneous separation of M. chinensis and simultaneous separation, which is applied to chemical instruments and methods, medical preparations containing active ingredients, organic active ingredients, etc., and can solve the problem of undiscovered chemical components of M. chinensis flowers and the like. , to achieve the effect of fast separation
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Embodiment 14
[0031] The preparation of embodiment 14 kinds of flavonoid components
[0032] (1) Preparation of crude extract
[0033] Weigh 10g of Meconopsis whole-leaf, pulverize with a powder machine, the powder is not larger than 0.3mm (basically all pass through No. 3 sieve), add 70% ethanol (v / v) 200mL, ultrasonic (45kHz, 300w) extraction 3 times, The filtrates were combined for 45 minutes each time, and the ethanol was recovered under reduced pressure to obtain a concentrated solution. The concentrated solution was placed in a -80°C refrigerator overnight, and then freeze-dried to obtain 3.11 g of dry extract, with a dry extract yield of 31.1%.
[0034] Take about 150mg of the extract, dissolve it in 50mL of water, transfer it to a separatory funnel, extract it with ethyl acetate and n-butanol in sequence, combine the ethyl acetate and n-butanol extracts, recover the solvent under reduced pressure at 45°C, and obtain the crude extract for later use .
[0035] (2) Separation and pur...
Embodiment 22
[0055] Preparation of 22 known flavonoid components of embodiment
[0056] (1) Preparation of crude extract
[0057] Weigh 10g of Meconopsis whole-leaf, pulverize with a powder machine, the powder is not larger than 0.3mm (basically all pass through No. 3 sieve), add 70% ethanol (v / v) 200mL, ultrasonic (45kHz, 300w) extraction 3 times, The filtrates were combined for 45 minutes each time, and the ethanol was recovered under reduced pressure to obtain a concentrated solution. The concentrated solution was placed in a -80°C refrigerator overnight, and then freeze-dried to obtain 3.11 g of dry extract, with a dry extract yield of 31.1%.
[0058] Take about 150mg of the extract, dissolve it in 50mL of water, transfer it to a separatory funnel, extract it with ethyl acetate and n-butanol in sequence, combine the ethyl acetate and n-butanol extracts, recover the solvent under reduced pressure at 45°C, and obtain the crude extract for later use .
[0059] (2) Separation and purific...
Embodiment 3
[0066] Embodiment 3 Research on antioxidant activity of compounds of the present invention
[0067] 1 material
[0068] General Analysis TU-1950 UV Spectrophotometer (Beijing General Analysis Company); METTLERAE240 Electronic Balance (Mettler-Toledo (Shanghai) Co., Ltd.), KQ-250B Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).
[0069] DPPH (Tixiai (Shanghai) Chemical Industry Development Co., Ltd.); ascorbic acid (Guangdong Guanghua Science and Technology Co., Ltd.); BHT (Chengdu Kelong Chemical Reagent Factory), other reagents were of analytical grade, and water was distilled water. Experimental sample: compound 1quercetin-3-O-β-D-glucopyrannosy-(1→6)-β-D-glucopyranoside, compound 2quercetin3-O-[2”'-O-acetyl-β-D-glucopyranosyl-(1 →6)-β-D-glucopyranoside, compound 3quercetin3-O-[6"'-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside, compound 4quercetin3-O-[3" '-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside. The above experimental samples were separ...
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