Method for separating and purifying kinds of flavonoid constituents from meconopsis integrifolia flowers at same time

A technique for simultaneous separation of M. chinensis and simultaneous separation, which is applied to chemical instruments and methods, medical preparations containing active ingredients, organic active ingredients, etc., and can solve the problem of undiscovered chemical components of M. chinensis flowers and the like. , to achieve the effect of fast separation

Inactive Publication Date: 2015-12-09
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is recorded in Tibetan medical books that Meconopsis whole-leaves uses the flowers as medicine, but no literature has been found to study the chemical constituents of Meconopsis whole-leaf flowers.
At present, there is no study on the separation and purification of flavonoids in Meconopsis lentilis by HSCCC method

Method used

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  • Method for separating and purifying kinds of flavonoid constituents from meconopsis integrifolia flowers at same time
  • Method for separating and purifying kinds of flavonoid constituents from meconopsis integrifolia flowers at same time
  • Method for separating and purifying kinds of flavonoid constituents from meconopsis integrifolia flowers at same time

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Experimental program
Comparison scheme
Effect test

Embodiment 14

[0031] The preparation of embodiment 14 kinds of flavonoid components

[0032] (1) Preparation of crude extract

[0033] Weigh 10g of Meconopsis whole-leaf, pulverize with a powder machine, the powder is not larger than 0.3mm (basically all pass through No. 3 sieve), add 70% ethanol (v / v) 200mL, ultrasonic (45kHz, 300w) extraction 3 times, The filtrates were combined for 45 minutes each time, and the ethanol was recovered under reduced pressure to obtain a concentrated solution. The concentrated solution was placed in a -80°C refrigerator overnight, and then freeze-dried to obtain 3.11 g of dry extract, with a dry extract yield of 31.1%.

[0034] Take about 150mg of the extract, dissolve it in 50mL of water, transfer it to a separatory funnel, extract it with ethyl acetate and n-butanol in sequence, combine the ethyl acetate and n-butanol extracts, recover the solvent under reduced pressure at 45°C, and obtain the crude extract for later use .

[0035] (2) Separation and pur...

Embodiment 22

[0055] Preparation of 22 known flavonoid components of embodiment

[0056] (1) Preparation of crude extract

[0057] Weigh 10g of Meconopsis whole-leaf, pulverize with a powder machine, the powder is not larger than 0.3mm (basically all pass through No. 3 sieve), add 70% ethanol (v / v) 200mL, ultrasonic (45kHz, 300w) extraction 3 times, The filtrates were combined for 45 minutes each time, and the ethanol was recovered under reduced pressure to obtain a concentrated solution. The concentrated solution was placed in a -80°C refrigerator overnight, and then freeze-dried to obtain 3.11 g of dry extract, with a dry extract yield of 31.1%.

[0058] Take about 150mg of the extract, dissolve it in 50mL of water, transfer it to a separatory funnel, extract it with ethyl acetate and n-butanol in sequence, combine the ethyl acetate and n-butanol extracts, recover the solvent under reduced pressure at 45°C, and obtain the crude extract for later use .

[0059] (2) Separation and purific...

Embodiment 3

[0066] Embodiment 3 Research on antioxidant activity of compounds of the present invention

[0067] 1 material

[0068] General Analysis TU-1950 UV Spectrophotometer (Beijing General Analysis Company); METTLERAE240 Electronic Balance (Mettler-Toledo (Shanghai) Co., Ltd.), KQ-250B Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0069] DPPH (Tixiai (Shanghai) Chemical Industry Development Co., Ltd.); ascorbic acid (Guangdong Guanghua Science and Technology Co., Ltd.); BHT (Chengdu Kelong Chemical Reagent Factory), other reagents were of analytical grade, and water was distilled water. Experimental sample: compound 1quercetin-3-O-β-D-glucopyrannosy-(1→6)-β-D-glucopyranoside, compound 2quercetin3-O-[2”'-O-acetyl-β-D-glucopyranosyl-(1 →6)-β-D-glucopyranoside, compound 3quercetin3-O-[6"'-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside, compound 4quercetin3-O-[3" '-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside. The above experimental samples were separ...

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Abstract

The invention provides a method for separating and purifying kinds of flavonoid constituents from meconopsis integrifolia flowers at the same time and a method for separating and purifying two kinds of known flavonoid constituents at the same time. Through an HSCCC method, four kinds of flavonoid constituents are successfully separated and purified from the meconopsis integrifolia flowers; the separation speed is high, and the method lays a foundation for material basis research of the meconopsis integrifolia flowers. The research finds that the separated known compounds or new compounds have good free radical scavenging activity and can be used as antioxidant medicine or a health-care product.

Description

technical field [0001] The invention relates to a method for simultaneously separating and purifying multiple flavonoid components from Meconopsis whole margin leaves. Background technique [0002] Meconopsis integrifolia (Maxim.) Franch. is a plant of the genus Meconopsis in the family Papaveraceae. It is a traditional Tibetan medicinal material. It is mainly distributed in Tibet, Sichuan, Yunnan, Qinghai, Gansu and other provinces in China. . "Jingzhu Materia Medica" records that Meconopsis whole-leaf is used as medicine to treat hepatitis, pneumonia and edema [1-2] . Modern chemical composition research has systematically studied the chemical composition of the above-ground parts of Meconopsis entire margin, using traditional separation methods to separate compounds such as alkaloids and flavonoids [3-5] . However, Tibetan medical books record that Meconopsis lentilis uses flowers as medicine, but no literature reports have been found on the chemical constituents of M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P39/06
Inventor 刘圆黄艳菲韩亚涛李伯超
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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