Method for quickly detecting lead poisoning based on magnetic separation and quantum dots marks and kit thereof

A kit and rapid technology, applied in measurement devices, analytical materials, instruments, etc., to achieve the effects of high photochemical stability, easy operation, and not easy to photolysis
CN103698527AInactive Publication Date: 2014-04-02CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Publication Date
2014-04-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for quickly detecting lead in liquid biological samples based on magnetic separation and quantum dots (QDs) marks. The method comprises the steps of (1), preparing a lead-resisting monoclonal antibody; (2), coupling the lead-resisting monoclonal antibody with nano magnetic beads through covalent bonds to prepare lead-resisting immune nano magnetic beads; and (3), adding bifunctional chelating agents, BSA (Bovine Serum Albumin) and triethylamine (TEA) into the liquid biological samples, performing incubation and adding the lead-resisting immune nano magnetic beads to mix fully, then performing magnetic separation, adding biotinylation BSA antibodies and streptavidin quantum dots (QDs) into sediments obtained by magnetic separation, and performing fluorescence detection by using a fluorescence microplate. The invention also provides a kit for quickly detecting lead in liquid biological samples.
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Description

technical field

[0001] The invention belongs to the technical field of biology and new medicine. specifically. The invention provides a method for rapidly detecting lead content in blood based on magnetic separation and quantum dot labeling technology and a kit for rapidly detecting lead content in blood. Background technique

[0002] Lead (Pb) pollution has attracted much attention because of its persistence, bioaccumulation and toxicity, and has been one of the hot research topics in the international scientific community. In recent years, with the acceleration of industrialization and urbanization in my country, lead pollution is on the rise. The heavy metal lead that enters the environment through the discharge of industrial "three wastes" has even directly affected the food chain and the quality of agricultural products. In many places, the lead content in foods such as grains, vegetables, and fruits seriously exceeds the standard or is close to the critical value, wh...

Claims

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