2019-nCoVs detection method and kit based on surface enhanced Raman spectroscopy

Abstract

The invention relates to a 2019-nCoV detection method and kit based on surface-enhanced Raman spectroscopy, and provides a biomacromolecule detection method and kit based on surface-enhanced Raman spectroscopy. The method comprises the following steps: firstly, preparing iodide ion modified silver nanoparticle sol as a mixture 1; adding acetonitrile into the mixture 1 to obtain a mixture 2; adding a sample to be detected into the mixture 2 to obtain a mixture 3; adding a substance capable of providing calcium ions into the mixture 3 to obtain a mixture 4; and carrying out SERS detection on the mixture 4. The method can be applied to actual clinical detection, can promote the application of SERS in the field of virus detection, and improves the handling capacity of sudden and new viral infectious diseases.

Description

technical field [0001] The invention belongs to the technical field of biomacromolecule detection, and in particular relates to a biomacromolecule detection method based on surface-enhanced Raman spectroscopy, and also relates to a kit. Background technique [0002] Biomacromolecules refer to macromolecules such as proteins, nucleic acids, and polysaccharides that exist in living cells. The structures are very complex, but their basic structural units are not complicated. Protein molecules are long chains of amino acid molecules arranged in a certain order. Amino acid molecules are the constituent materials of most living matter, and there are dozens of different amino acid molecules. The vast majority of enzymes in organisms belong to proteins, which are indispensable for organisms to maintain normal metabolic functions. Raman spectroscopy is a powerful means to study biomacromolecules. It can study the structure and changes of biomacromolecules in close to natural state ...

AI Technical Summary

Problems solved by technology

However, since the "hot spots" of SERS technology are usually less than 10nm space between gold or silver nanostructures, while biomacromolecules are larger than 10nm, for example, the size of viruses is usually about 100nm, which makes viruses and other biomacromolecules unable to adapt to SERS. "Hot spots" cannot be reliably detected and identified simply by using traditional SERS technology. In view of the above, the following improvements have been made in the prior art:

However, the method used cannot obtain the fingerprint of the virus itself, but indirectly detects it through the protein expressed after it infects the cell, and the fingerprint signal of the protein is easily affected by the inherent protein on the cell surface, resulting in poor accuracy.

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