2019-nCoVs detection method and kit based on surface enhanced Raman spectroscopy
A surface-enhanced Raman and detection method technology, applied in reagent containers, Raman scattering, chemical instruments and methods, etc., can solve the problems of inability to obtain virus fingerprints, inability to reliably detect and identify, inability to adapt to hot spots, etc. The effect of reproducibility, high signal-to-noise ratio, and high sensitivity
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[0060] Example 1
[0061] The silver-soluble with citrate ions in accordance with Lee, wherein the sodium citrate solution (1%, 4 mL) is added to a slightly boiling nitrate solution (0.034 g, 200 mL), and the silver nanoparticles are also reducing silver nanoparticles. Silver sol (6500 rpm, 20min, 20 ° C) of 5 ml of sodium citrate (6500 rpm, 20 min, 20 ° C), after removing the supernatant, 20 μl of centrifugal silver sol with 20 μl of the potassium iodide solution (1 mM) was incubated at room temperature to obtain an iodine ion-modified silver nanoparticle Sol;
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[0062] Example 2
[0063] (1) 20 μl of centrifugal silver sol with a mixture of 20 μl of potassium iodide solution (1 mm) was added 3 μl of acetonitrile;
[0064] (2) Further, 20 μl of D-influenza virus sample (100 copies / ml) and 2 μl of CA 2+ (0.01M CACL 2 · 2h 2 O) mix;
[0065] (3) The mixture is sufficiently mixed and placed on the support for SERS detection, the laser wavelength is 633 nm, the scan time is 35s, the laser energy is 28mW, and each test is accumulated once.
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[0066] Example 3
[0067] The difference from the embodiment 2 is that the viral sample is a C-influenza virus.
[0068] like figure 2 As shown, the above Examples 2 and 3 have obtained the SERS signal of the viral constituent substance through test verification, and can obtain a fingerprint spectrum of different types of influenza virus at very low (100 copies / ml),
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