The invention relates to a making method of radish seed wholemeal biscuits. The biscuits are made from whole wheat flour, radish seeds, wheat bran, vegetable oil, xylitol, oat, salt and a raising agent. According to the biscuits, the principle of nutrition and health is followed according to material selection, the radish seeds, wheat bran, oat and xylitol are added particularly, various nutrientsand dietary fiber are contained, and particularly the radish seeds contain the unique matter called raphanin which has the antitumor activity. Due to the ratio of the raw materials, the requirement for the taste is met, and the effects of promoting digestion, enhancing gastrointestinal tract power, preventing decayed teeth, lowering blood pressure and the like are achieved. The radish seed wholemeal biscuits are unique in flavor and abundant in nutrient, and the making method is simple and easy to master, and suitable for large-scale production.