Preparation method of molecular engram composite film for separating tryptophane isomer

A technology of molecular imprinting and tryptophan, applied in semipermeable membrane separation, chemical instruments and methods, membrane technology, etc., can solve the problems of binding stability and poor compatibility between base membrane and functional groups, and achieve separation effect. Significant, fast separation, good mechanical properties

Active Publication Date: 2012-11-07
陕西省膜分离技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the commonly used synthetic polymer membrane materials such as nylon, polysulfone, polyvinylidene fluoride, etc. are generally hydrophobic materials, and have poor compatibility with hydrophilic chitosan, such composite membranes often have base membrane and The problem of binding stability between functional bases

Method used

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  • Preparation method of molecular engram composite film for separating tryptophane isomer
  • Preparation method of molecular engram composite film for separating tryptophane isomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Step 1, preparing polyvinylidene fluoride (PVDF) / polyvinyl alcohol (PVA) blended ultrafiltration base membrane:

[0037] (1) Preparation of casting solution: mix 20 parts by mass of PVDF / PVA mixed resin material (the mass ratio of PVDF to PVA is 4 / 1, the molecular weight range of PVDF is 400~600KDa, and the molecular weight range of PVA is 30~15KDa , and the hydroxyl hydrolysis degree of PVA ≥ 98%) and 3 parts by mass of anhydrous lithium chloride were added to 77 parts by mass of dimethylformamide, mixed at 90°C and placed for 12 hours to obtain a casting solution;

[0038] (2) Preparation of nascent basement membrane: Pour the casting liquid onto a flat and clean glass plate, and scrape the nascent basement membrane with a uniform and smooth round rod-shaped steel member with 0.3mm copper wires fixed on both sides;

[0039] (3) Gel phase separation: After the nascent basement membrane stays in the air for 30s, it is immersed in deionized water at 40°C to form a gel ph...

Embodiment 2

[0050] The difference from Example 1 is that vinylidene fluoride and polyvinyl alcohol resin material (wherein PVDF / PVA=6 / 1 (mass ratio) with mass parts of 16, and lithium chloride with mass parts of 5, and The organic solvent dimethylformamide with a mass fraction of 68 was blended and dissolved to prepare a PVDF / PVA blended ultrafiltration membrane with a pure water flux of 128L / h m 2 , BSA retention rate is 99.5%.

[0051] Using 2% (v / v) acetic acid aqueous solution as solvent, prepare chitosan 3% (w / w), D-tryptophan 0.75% (w / w), glutaraldehyde 0.3% (w / w) For the coating solution, adjust the pH to 2 with hydrochloric acid, and pour the coating solution into a tetrafluoroethylene dish covered with a polyvinylidene fluoride / polyvinyl alcohol (PVDF / PVA) blended base film at the bottom to soak the film surface by about 0.2 mm, after standing at room temperature for defoaming, put the film in an oven at 50°C until the film is basically dry. After removing the film, rinse the re...

Embodiment 3

[0054] The conditions of the base film in this example are the same as in Example 1. The concentration of chitosan in the coating solution is 5% (w / w), the template L-tryptophan is 1.25% (w / w), the concentration of glutaraldehyde is 0.2% (w / w), pH=2, membrane preparation And elution method is with example 1.

[0055] Under the same osmotic conditions as in Example 1, the osmotic separation factor of the obtained chitosan molecularly imprinted composite membrane was 2.21.

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Abstract

The invention discloses a preparation method of a molecular engram composite film for separating a tryptophane isomer, which comprises the following steps: by using a polyvinylidene fluoride/polyvinyl alcohol blended film as the base film, coating chitosan used as a functional polymer molecular engram coating liquid on the surface of the base film in a crosslinking manner; and after the solvent is volatilized to dryness, eluting to remove template molecules, thus obtaining the molecular engram composite film capable of performing specific recognition on the template molecules. The composite film prepared by the method can continuously and fast realize the separation of a tryptophane isomer, has the advantages of simple separation operation, high efficiency and energy saving, and can be easily industrialized.

Description

technical field [0001] The invention belongs to the technical field of membrane chiral separation, and in particular relates to a preparation method of a molecularly imprinted composite membrane for separating tryptophan isomers. Background technique [0002] Chitosan was earlier used as a functional material for chiral separation of amino acids. CN00122948.6 discloses a molecular imprinted membrane preparation method for chitosan / silicon dioxide hybrid membrane for chiral separation of amino acids. Its chiral recognition function has its unique role in the separation of amino acids by membrane imprinting technology. However, chitosan dissolves in acidic solution, which easily causes damage to the mechanical properties of the membrane and deformation of the molecularly imprinted sites. [0003] CN200810220537 discloses a method for separating chiral substances by chemically derivatizing polysaccharides into solvent-resistant functional groups. This method shows that polysac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D71/38B01D69/12B01D67/00C07D209/20
CPCY02P20/50
Inventor 孟晓荣杨琴张楠杨青翠张海珍
Owner 陕西省膜分离技术研究院有限公司
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