Sesame peptide powder, and preparation method and application thereof

A technology for sesame peptides and sesame seeds, applied in the field of preparation and enzymolysis, can solve the problems of harsh preparation conditions of sesame peptides, high cost of gel chromatography columns, unsuitable for large-scale production, etc., and achieves saving drying time, low cost, and adding small amount of effect

Active Publication Date: 2020-01-10
SINPHAR TIAN LI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this method, n-hexane is used as a degreasing solvent, and n-hexane is a flammable and explosive solvent, which brings safety hazards to industrial production, and n-hexane will remain in the product, causing harm to the human body. The protein determination method detects sesame peptides, so the peptide content is inaccurate and unscientific
Wang Zhenbin and others used ultrasonic and microwave technology to assist enzymatic hydrolysis to obtain sesame peptides. Ultrasonic and microwave technologies are not suitable for large-scale production, resulting in limitations in the use of the method
Li Jia et al. used alkaline protease to hydrolyze the sesame protein, then used gel chromatography to separate and purify to obtain antioxidant peptides. The gel chromatography column was expensive and the post-processing was complicated, which made it difficult to produce on a large scale.
It can be seen that the preparation conditions of the above sesame peptides are harsh, the process steps are cumbersome, and it is difficult to achieve large-scale production. Therefore, finding a set of preparation methods for sesame peptides with simple process, low cost, high content, and high activity has become a search for large-scale production. One of the research hotspots

Method used

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  • Sesame peptide powder, and preparation method and application thereof
  • Sesame peptide powder, and preparation method and application thereof
  • Sesame peptide powder, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0046] Cold-pressed and deoiled black sesame to obtain defatted sesame meal, weighed 100 kg of sesame meal, recorded as A, added 1000 L of water, adjusted pH to 8.0-8.5, and extracted at room temperature for 1 hour; Pour the same amount of sesame meal, record it as B, adjust the pH to 8.0-8.5 and extract at room temperature for 1 hour; after B completes the first extraction, the filtrate is set aside for use, and the filter residue continues for the second extraction; after A completes the second extraction, The filter residue was discarded, the filtrate was poured into the same amount of sesame meal, recorded as C, the pH was adjusted to 8.0-8.5, and the extraction was performed at room temperature for 1 hour; after B completed the secondary extraction, the filter residue was discarded, and the filtrate was poured into C to complete the filter residue of the first extraction. After the second extraction of sample C, the filter residue was discarded, and the filtrate was set as...

preparation Embodiment 2

[0048] Cold-pressed and deoiled black sesame to obtain defatted sesame meal, weighed 100kg of sesame meal, recorded as A, added 500 L of water, adjusted pH to 9.0-9.5, and extracted at room temperature for 1 hour; Pour the same amount of sesame meal, record it as B, adjust the pH to 9.0-9.5 and extract at room temperature for 1 hour; after B completes the first extraction, the filtrate is set aside for use, and the filter residue continues for the second extraction; after A completes the second extraction, The filter residue was discarded, the filtrate was poured into the same amount of sesame meal, recorded as C, and the pH was adjusted to 9.0-9.5 for extraction at room temperature for 1 hour; after B completed the secondary extraction, the filter residue was discarded, and the filtrate was poured into C to complete the filter residue of the first extraction. After the second extraction of sample C, the filter residue was discarded, and the filtrate was set aside for use; fina...

preparation Embodiment 3

[0050] Cold-pressed and deoiled black sesame to obtain defatted sesame meal, weighed 100 kg of sesame meal, recorded as A, added 500 L of water, adjusted the pH to 8.5-9.0, and extracted at room temperature for 0.5 h; Pour the filtrate into the same amount of sesame meal, record as B, adjust the pH to 8.5-9.0 and extract at room temperature for 0.5h; after B completes the first extraction, the filtrate is ready for use, and the filter residue continues for the second extraction; A completes the second extraction Then, the filter residue was discarded, the filtrate was poured into the same amount of sesame meal, recorded as C, the pH was adjusted to 8.5 to 9.0, and the extraction was performed at room temperature for 0.5h; after B completed the secondary extraction, the filter residue was discarded, and the filtrate was poured into C to complete the first time The extracted filter residue was extracted for 0.5h, and the filtrate of the first extraction of C was for use; after th...

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Abstract

The invention relates to sesame peptide powder, and a preparation method and application thereof. The peptide content of the sesame peptide powder is 90wt% or above, wherein the molecular weight of more than 90% of sesame peptide is less than 1000 Dalton. Degreased sesame meal is subjected to protein extraction and enzymolysis, purified, concentrated and dried to obtain the sesame peptide. The sesame peptide powder has good free radical removing activity, improves the sleep quality, enhances human immunity and meanwhile has an anti-haze effect. The preparation method is easy to operate, high in yield, low in cost, stable in product quality, environmentally friendly in process and suitable for large-scale production.

Description

technical field [0001] The invention relates to a high-purity, low-molecular-weight sesame peptide product, wherein the peptide content is over 90 wt %, and the molecular weight is less than 1000 Dalton and over 90 percent. The invention also relates to the preparation and enzymatic hydrolysis of sesame protein to generate the preparation method of oligomeric sesame peptide powder, and the peptide powder can be used as food, health food or medicine. Background technique [0002] Sesame (Sesamum indicm L.) belongs to the Flax family and is a seed species of flax, distributed in tropical regions and some temperate regions. Sesame contains a lot of fat and protein, and is also rich in sterols, sesamin, sesamol, lecithin compounds, as well as dietary fiber, carbohydrates, vitamins and minerals. Sesame seeds can be divided into four categories according to color, namely white sesame, black sesame, yellow and white sesame, and variegated sesame. Among them, black sesame has the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K4/10C07K1/34C07K1/14C12P21/06A61K38/01A61K8/64A61P25/20A61P37/04A61P11/00A61P1/00A23L33/18
CPCA23V2002/00A61K8/645A61K38/00A61K2800/10A61K2800/805A23L33/18A61P1/00A61P11/00A61P25/20A61P37/04C07K4/10C12P21/06A23V2200/314A23V2250/55Y02P20/54
Inventor 王昭日刘明川杨胜杰
Owner SINPHAR TIAN LI PHARMA
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