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Preparation method of blueberry leaf polyphenol having anti-oxidation activity

A technology of blueberry leaf polyphenols and antioxidant activity, which can be applied to medical preparations containing active ingredients, anti-toxins, pharmaceutical formulas, etc., and can solve the problems of lack of research on blueberry leaf polyphenols

Inactive Publication Date: 2017-09-29
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, researchers focus on blueberry fruit, and the research on polysaccharides, anthocyanins, flavonoids and volatile aroma components contained in blueberry fruit has been very mature, but the research on blueberry leaf polyphenols is relatively scarce

Method used

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  • Preparation method of blueberry leaf polyphenol having anti-oxidation activity
  • Preparation method of blueberry leaf polyphenol having anti-oxidation activity
  • Preparation method of blueberry leaf polyphenol having anti-oxidation activity

Examples

Experimental program
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Embodiment 1

[0031] 1) Rinse, dry, and pulverize the picked blueberry leaves into powder and pass through a 40-mesh sieve;

[0032] 2) Add the blueberry leaf powder into 60% ethanol aqueous solution, the ratio of solid to liquid is 1:30, and extract by ultrasonic wave for 20 minutes, the temperature is 30°C, the ultrasonic power is 500W, and the frequency is 40kHz;

[0033] 3) Filter the mixture in step 2) through 8 layers of gauze to remove the residue to obtain crude polyphenol extract 1;

[0034] 4) centrifuge the polyphenol crude extract 1 at 5000 g for 15 min, and take the supernatant to obtain a light yellow polyphenol crude liquid 2;

[0035] 5) Pour the polyphenol crude liquid 2 into a rotary steaming bottle for rotary steaming, and stop the rotary steaming after the solution turns into a yellow-brown thick liquid;

[0036] 6) Freeze-dry the rotary distillate obtained in step 5) to obtain crude blueberry leaf polyphenol extract and store it in a -20°C refrigerator for future use. ...

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Abstract

The invention discloses a preparation method of blueberry leaf polyphenol having anti-oxidation activity. The method comprises the steps of ultrasonic extraction, filtration, centrifugation, taking of the obtained supernatant, vacuum concentration and HPD 400 type macro-porous resin column chromatographic purification. The free radical scavenging activities of crude blueberry leaf polyphenol extract and the blueberry leaf polyphenol purified through the HPD 400 type macro-porous resin purification are evaluated through an ABTS technology and a DPPH technology, and rutin us as a reference substance. The preparation method of the blueberry leaf polyphenol has the characteristics of high extraction efficiency, good repeatability, mild conditions and no pollution, and the prepared blueberry leaf polyphenol has significant anti-oxidation activity, so the blueberry leaf polyphenol has a wide application prospect in the food industry and the pharmaceutical industry.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, in particular to a method for extracting high-purity polyphenols from blueberry leaves Background technique [0002] Blueberry, also known as Dusi lingonberry, blueberry, etc., is native to North America and belongs to the Rhododendronaceae Vaccinium subfamily Vaccinium perennial deciduous or evergreen shrubs according to botanical classification. Blueberry leaves are higher in polyphenols than blueberry fruit. The content of polyphenols in blueberry leaves of Rabbiteye blueberry in Nanjing area was as high as 81.82mg gallic acid equivalent / g dry weight. The polyphenols of blueberry leaves mainly include caffeoylquinic acid, catechin and its polymers, quercetin and its glycoside derivatives, kaempferol and its glycoside derivatives, etc. Blueberry leaf polyphenols are the main physiologically active substances in blueberry leaves. They have anti-oxidation, anti-tumor, anti-virus, blood...

Claims

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Application Information

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IPC IPC(8): A61K36/45A61P39/06A23L33/105
CPCA23L33/105A23V2002/00A61K36/45A61K2236/333A61K2236/51A61K2236/53A61K2236/55A23V2200/30A23V2250/2106A23V2300/14A23V2300/48A23V2300/02A23V2300/10
Inventor 李春阳冯进王帆闫征吴寒
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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