Isobenzofuran compound with effect of removing free radicals in radix puerariae and application of isobenzofuran compounds in tobacco

A compound and furan technology, applied in the field of isobenzofuran compounds and their preparation, to achieve the effect of simple structure

Active Publication Date: 2017-06-23
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effect of lactone compounds found in kudzu root to scavenge free radicals in cigarette smoke has not been reported in the relevant literature

Method used

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  • Isobenzofuran compound with effect of removing free radicals in radix puerariae and application of isobenzofuran compounds in tobacco
  • Isobenzofuran compound with effect of removing free radicals in radix puerariae and application of isobenzofuran compounds in tobacco
  • Isobenzofuran compound with effect of removing free radicals in radix puerariae and application of isobenzofuran compounds in tobacco

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preparation example Construction

[0030] The preparation method of the isobenzofuran compound of the present invention uses the roots of Pueraria lobata root as a raw material, and undergoes the steps of extract extraction, organic solvent extraction, MCI decolorization, silica gel column chromatography, and high performance liquid chromatography preparation and separation steps. :

[0031] A. Extraction extraction: crush the roots of Pueraria lobata to 20-40 mesh, and ultrasonically extract 2 to 5 times with an organic solvent for 30 to 60 minutes each time. Combine the extracts, filter, concentrate the extracts under reduced pressure, and leave them to stand. Filter off the precipitate and concentrate it into extract a;

[0032] B. Organic solvent extraction: add 1 to 2 times the weight of water to extract a, then extract 3 to 5 times with an organic solvent equal in volume to water, combine the organic solvent extraction phases, and concentrate under reduced pressure to extract b ;

[0033] C. MCI decolorization...

Embodiment 1

[0044] Take 4.4 kg of dried Pueraria lobata, coarsely crush it to 30 mesh, and extract 4 times with 70% acetone ultrasonically, 60 minutes each time. The extracts are combined; the extracts are filtered and concentrated under reduced pressure to 1 / 4 of the volume; Filter off the precipitate and concentrate it into 120 g of extract a; add 250 g of water to extract a, extract 5 times with the same volume of chloroform as water, combine the extract phases, and concentrate under reduced pressure to 80 g of extract b; Paste b is packed in MCI column, and 240 g of 80% methanol water is added to the extract b to dissolve it, and then it is applied to the column and eluted with 2 to 6 liters of 90% methanol water. The eluate is collected and concentrated under reduced pressure to obtain 62 g. Paste c; Extract c Add 120 g of acetone to extract c to dissolve, then add 62 g of 100 mesh silica gel and mix the sample. After mixing, use 200 mesh silica gel 400 g to pack the column; the volume...

Embodiment 2

[0046] Take 10 kg of dried kudzu root block, coarsely crush it to 40 mesh, and extract 4 times with 80% methanol cold soaking for 3 days each time. The extracts are combined; the extracts are filtered and concentrated under reduced pressure to 1 / 4 of the volume; , Filter off the precipitate and concentrate it into 300 g extract a; add 350 g of water to extract a, extract 5 times with ethyl acetate equal to the volume of water, combine the extract phases, and concentrate under reduced pressure to 210 g extract b The extract b is packed with MCI column, 600 g of 80% methanol water is added to the extract b to dissolve, and then the column is used for elution with 5 to 15 liters of 90% methanol water. The eluent is collected and concentrated under reduced pressure to obtain 150 g extract c; add 300 g of acetone to extract c to dissolve it, then add 150 g of 100 mesh silica gel to mix the sample, use 200 mesh silica gel 1 Kg to pack the column, mix the sample and load the column; th...

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Abstract

The invention discloses an isobenzofuran compound as well as a preparation method and application thereof. The isobenzofuran compound is obtained by separating from a traditional Chinese herbal medicine radix puerariae; the compound is named as 5-methoxy-2,2-dimethyl-2H-furo[3,4-g] chromen-8(6H)-one as an English name, and a molecular formula of the compound is C14H14O4; a structural formula of the compound is shown in the description. The preparation method of the isobenzofuran compound comprises the following steps: using the traditional Chinese herbal medicine radix puerariae as a raw material and carrying out extractum extraction, organic solvent extraction, MCI decolorizing, silica-gel column chromatography and high pressure liquid chromatography separation to obtain a finished product; the isobenzofuran compound is subjected to antioxidant activity and free radical removal activity screening, so the isobenzofuran compound has higher antioxidant and free radical removing activities; the compound is used for producing cigarettes and can be used for effectively removing the free radicals in smoke gas of the cigarettes.

Description

Technical field [0001] The invention belongs to the technical field of phytochemistry, and specifically relates to an isobenzofuran compound obtained by first extracting from the traditional Chinese medicinal material Pueraria lobata root, and a preparation method and application thereof. Background technique [0002] Pueraria lobata is the dried root of the leguminous plant Pueraria lobata, commonly known as Pueraria lobata. Excavated in autumn and winter, cut into thick slices or small pieces while fresh; dry. Sweet, pungent and cold. It has the functions of relieving muscles and reducing fever, removing rash, producing body fluids and quenching thirst, promoting Yang and stopping diarrhea. It is often used for symptoms of fever, strong nape and back pain, impervious measles, thirst due to fever, yin deficiency and thirst, yin deficiency and dysentery, heat diarrhea, spleen deficiency and diarrhea. Pueraria lobata root is also an important health food with high medicinal val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A24B15/40
CPCA24B15/406C07D493/04
Inventor 李干鹏王明锋者为陈章玉吴海燕周敏蒋薇杨青松胡秋芬杨光宇刘志华陈永宽
Owner YUNNAN MINZU UNIV
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