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Method of extracting flavonoid glycosides from grosvenor monordica leaves and product thereof

A technology of Luo Han Guo leaves and flavonoid glycosides is applied in chemical instruments and methods, preparation of sugar derivatives, antidote and other directions, which can solve the problems of not using, throwing away, and extracting flavonoid glycosides monomers, etc., so as to improve the utilization rate and reduce the Waste, the effect of simple extraction process

Inactive Publication Date: 2012-05-23
杨国栋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The source of Luo Han Guo leaves is sufficient. Only the planting area of ​​Luo Han Guo in Guilin, Guangxi has exceeded 10,000 mu, and each mu can produce 200 kilograms of leaves. However, a large number of Luo Han Guo leaves are cut off in autumn and then thrown away or burned without being used.
However, there is no relevant report on the extraction of flavonoid glycoside monomers from Luo Han Guo leaves and the research on their monomer activity.

Method used

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  • Method of extracting flavonoid glycosides from grosvenor monordica leaves and product thereof
  • Method of extracting flavonoid glycosides from grosvenor monordica leaves and product thereof
  • Method of extracting flavonoid glycosides from grosvenor monordica leaves and product thereof

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Embodiment 1

[0020] Embodiment 1 of the present invention: the method for extracting flavonoid glycoside composition (kaempferol-3,7-a-L-dirhamnoside) from Luo Han Guo leaves: get Luo Han Guo leaves 100g, add 30 times of weight of water to boil and extract 3 times, Extract for 50 minutes each time, filter, combine the filtrates, enter the D101 macroporous resin for adsorption, wash the resin until the effluent is colorless, then elute with 75% ethanol, collect the eluate and remove the ethanol, and place it in a refrigerator at 4-10°C After 24 hours, a precipitate was precipitated, dissolved in absolute ethanol, filtered, and the filtrate was evaporated to dryness to obtain a crude extract of kaempferol-3,7-a-L-dirhamnoside; the purity of the crude extract was 65% (as determined by HPLC), and the yield 2%. The crude extract was subjected to silica gel column chromatography, and gradient elution was carried out with a solution with a volume ratio of chloroform and methanol of 20:1 to 1:1, a...

Embodiment 2

[0021] Embodiment 2 of the present invention: the method for extracting flavonoid glycoside composition (kaempferol-3,7-a-L-dirhamnoside) from Luo Han Guo leaf: get Luo Han Guo leaf, add 20 times of weight water to boil and extract 2 times, every Extract for 80 minutes, filter, combine the filtrates, enter D101 macroporous resin for adsorption, wash the resin with water until the effluent is colorless, then elute with 50% ethanol, collect the eluate and remove ethanol, and place it in a refrigerator at 4-10°C for 30 hours , precipitate out, dissolve with dehydrated ethanol, filter, and the filtrate is evaporated to dryness to obtain kaempferol-3,7-a-L-dirhamnoside crude extract; crude extract purity 62% (HPLC assay), yield is 1.8%. The crude extract was subjected to silica gel column chromatography, and gradient elution was carried out with a solution of chloroform and methanol with a volume ratio of 20:1 to 1:1, and the eluted part at 5:1 was collected, left to stand, and cry...

Embodiment 3

[0022] Embodiment 3 of the present invention: the method for extracting flavonoid glycoside composition (kaempferol-3,7-a-L-dirhamnoside) from Luo Han Guo leaf: get Luo Han Guo leaf, add 15 times of weight water and boil and extract 3 times, every Extract for 90 minutes, filter, combine the filtrates, enter the macroporous resin for adsorption, wash the resin until the effluent is colorless, then elute with anhydrous methanol, collect the eluate and remove the methanol, place it in the refrigerator for 20 hours, precipitate out, and use for precipitation Dissolve in absolute ethanol, filter, and evaporate the filtrate to dryness to obtain a crude extract of kaempferol-3,7-a-L-dirhamnoside; the purity of the crude extract is 63% (as determined by HPLC), and the yield is 1.5%. The crude extract is subjected to silica gel column chromatography, and gradient elution is carried out with a solution of chloroform and methanol with a volume ratio of 20:1 to 1:1, and the eluted part of ...

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Abstract

The invention discloses a method of extracting flavonoid glycosides from grosvenor monordica leaves and a product thereof. The method comprises the following steps: taking Grosvenor monordica leaves, boiling the Grosvenor monordica leaves with water, carrying out extraction and filtration twice to three times, each extraction lasting 50 to 100 min, combining filtrates, carrying out adsorption on an obtained filtrate by using a macroporous resin, rinsing the resin with water until effluent is colorless, carrying out elution by using ethanol or absolute methanol, collecting eluent, removing ethanol or methanol, placing the eluent in a refrigerator for 20 to 30 h, allowing precipitate to deposit, dissolving the precipitate with absolute ethanol, carrying out filtration and evaporating an obtained filtrate so as to obtain a crude extract, kaempferol-3,7-a-L-dirhamnoside. The extraction method provided in the invention is simple, scientific and reasonable, has strong feasibility and enables an obtained extract product to have a high purity, wherein, the purity of a refined extract product is higher than 95%; the extract product obtained in the invention has good free radical scavenging activity and a wide application prospect. In addition, application of the method enables full utilization of considerable waste grosvenor momordica leaves to be realized, thereby improving the utilization rate of natural resources and reducing waste.

Description

technical field [0001] The invention relates to a method for extracting flavonoid glycosides from Luo Han Guo leaves and its products, in particular to a method for extracting kaempferol-3,7-a-L-dirhamnoside and its products, belonging to the technical field of extracting medicinal active ingredients. Background technique [0002] As a traditional specialty in northern Guangxi, Luo Han Guo is also a unique medicinal plant in my country. It is mainly distributed in Guangdong, Guangxi, Yunnan, Jiangxi, and southern Hunan. It is mainly produced in Yongfu, Lingui, and Longsheng counties in Guangxi. Luo Han Guo usually grows in the damp and cool areas in the mountains. There are 7 species of plants in the same genus, and there are 4 species in my country. It has similar efficacy in treating diseases of the respiratory system and digestive system. Luo Han Guo is rich in flavonoid glycosides, mannitol, vitamin E, etc., and has good effects in cancer prevention and beauty. This mak...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08A61P39/06
Inventor 杨国栋
Owner 杨国栋
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