New coumarin active content, preparation method and application thereof

A technology of coumarins and biologically active ingredients is applied to a new field of coumarin-based active ingredients and their preparation and application, which can solve the problem that the research on compounds, active ingredients, and the mechanism of action is not clear enough, which affects further applications. And other issues

Active Publication Date: 2010-01-06
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, pigeonpea leaves have been used more and more widely as a Chinese herbal medicine, but the research on active ingredients and mechanism of action is not clear enough,

Method used

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  • New coumarin active content, preparation method and application thereof
  • New coumarin active content, preparation method and application thereof
  • New coumarin active content, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Isolation of novel coumarin-like active compounds in pigeon pea leaves

[0039] Weigh 5 kg of dried branches and leaves of pigeon pea and extract 3 times with 80% supercritical CO2 for 2 hours each time. After the extract is concentrated, add it to 40°C warm water (4L), vibrate and flocculate for 30min under the condition of 50KHz in an ultrasonic extractor, stand still for 30min, take the floc, add it with 2 times the volume of quartz sand and stir it into a glass column, then Add 8 / 1 (v / v) chloroform-water with a sample amount of 12mL / g, pass air at a flow rate of 0.25L / min, continue for 30min, after static stratification, recover the organic solvent to obtain the crude product of the target. Dissolve a small amount of methanol, add an equal mass of chromatographic silica gel with a mesh number of 300-400, mix well, and evaporate to dryness under reduced pressure at 40°C. Weigh 300-400 mesh silica gel with 4 times the mass of the extract in advance, use pe...

Embodiment 2

[0040] Example 2. DPPH free radical scavenging activity test

[0041] Add 100 μl of the sample solution to be tested and 3.5 ml of DPPH solution into the test tube, keep in a constant temperature water bath at 30°C for 20 min, take out and measure its absorbance at 520 nm, and use a blank solvent as a control. 5 concentrations were made for each sample, and 3 parallels were made for each concentration, and ascorbic acid was used as a positive control. Statistical processing of experimental results to calculate IC of radical scavenging activity 50 value.

Embodiment 3

[0042] Example 3. Antioxidant activity test of beta-carotene-linoleic acid

[0043] Add 100μl of the sample solution to be tested and 4.9ml of β-carotene-linoleic acid emulsion to the test tube, use the solvent as a blank control, and measure its absorbance at 470nm, which is the value at 0min, prepare a 50°C constant temperature water bath, and measure it well The absorbance at 0min immediately poured the test solution back into the test tube and put it into the water bath, and measured it every 15min until the β-carotene in the blank control tube faded, and the BHT solution was used as the positive control. Do 5 concentrations for each sample, and do 4 replicates for each concentration. Calculation of Antioxidant Activity IC 50 value. Table 2 shows the results of antioxidant activity in vitro.

[0044] Table 2 Pigalactone antioxidant activity IC 50

[0045]

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PUM

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Abstract

The invention relates to a new coumarin active content, i.e. pigeon pea lactone extracted and separated from cajan leaves, an extraction and separation method and an application thereof. The structure of the compound is shown as a figure 1. The invention adopts the technical scheme that agricultural waste of cajan leaves is used as raw material which is extracted by a solvent; the extracting solution is concentrated and dispersed in water; an ultrasonic oscillation flocculation technology, a bubble column extraction technology and a column chromatography separation technology in positive-phase silica gel are adopted and combined with a low-temperature crystallization and recrystallization technology to obtain the content with the purity higher than 95 percent; and the anti-oxidation activity and the action of the chemical content on cardiovascular diseases are detected by a DPPH model, a beta-renieratene-linoleic acid model, an in-texture SOD enzyme model, a platelet aggregation model induced by ADP in corpora and an aorta systole model induced by phenylephrine. Results show that the active content has favorable free radical scavenging activity, anti-oxidation activity in corpora and in vitro, platelet aggregation restraining action in corpora and a certain hemangiectasis action. Meanwhile, the preparation method of the pigeon pea lactone in the invention is easy and practical and can obtain a product with high purity high yield and high added value in a short period which is suitable for industrialized production and application, can be further developed and researched into a new antioxidant as an additive for medicines, foods and cosmetics.

Description

technical field [0001] The present invention relates to a kind of compound with biological activity extracted and separated from traditional Chinese medicine, in particular to a new kind of lactone compound with biological activity extracted and separated from leaves of traditional Chinese medicine pigeon pea [Cajanus cajan L.Millsp.] and its extraction method. The compound is pigeonolide with chemical name: 7-hydroxy-5-O-methyl-8-(3-methyl-2-butene)-4-phenyl-9,10-dihydro-benzene and pyran-2-one. Background technique [0002] With the development of social productivity, people are increasingly pursuing health and longevity, and finding new active ingredients from natural products is one of the important ways to develop new drugs to treat clinical diseases that endanger human health and reduce the occurrence of drug-derived diseases. The invention uses modern extraction, separation and purification means, combined with advanced physical means and chemical methods, to study ...

Claims

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Application Information

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IPC IPC(8): C07D311/16A61K31/37A61P9/00A61P17/18A61P17/16A61P39/06A61P25/28A61P9/10A61P9/12A61P7/02A61Q17/04A61Q19/08A23L1/30A61K36/48A61K127/00A23L33/105
CPCY02P20/54
Inventor 付玉杰祖元刚孔羽吴楠刘威祖柏实张琳顾成波李庆勇罗猛
Owner NORTHEAST FORESTRY UNIVERSITY
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