Sea asparagus ferulic acid ester compound as well as preparation method and application of compound
A technology of pentadecyl ferulate and ethyl acetate, which is applied in the field of sea asparagus ferulic acid ester compound and its preparation, can solve the problems of carcinogenicity, low toxicity, strong hydrophilicity and difficult antioxidant activity, etc. Achieve strong free radical scavenging activity and strong oxidation activity
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Embodiment 1
[0021] The preparation of embodiment 1 compound
[0022] 26.4kg of freshly picked sea asparagus was mashed with a homogenizer, placed in a 20L volumetric flask, added 8:2 acetone and water for extraction, left for 24 hours, and extracted three times. Suction filtration with a Buchner funnel, and the resulting solution was concentrated in vacuo at 30° C. with a rotary evaporator to obtain an acetone extract. Then, the acetone extract was extracted three times with ethyl acetate, and concentrated in vacuo at 30° C. with a rotary evaporator to obtain the ethyl acetate layer extract.
[0023] The ethyl acetate layer (71g) was mixed with 50g of silica gel in a dry method, then mounted on a normal-pressure column equipped with 200g of silica gel (300-400 mesh), and separated by petroleum ether-acetone-methanol gradient elution, that is, the elution of pure petroleum ether began. Gradually increase the proportion of acetone and decrease the proportion of petroleum ether until the el...
Embodiment 2
[0028] The test of embodiment 2 antioxidant activity
[0029] 1. Experimental samples and experimental methods
[0030] Preparation of positive reference substance: Weigh 0.1g of ascorbic acid, dissolve it in 100mL of distilled water to make a 1mg / mL solution, then pipette 0mL, 0.8mL, 1.6mL, 2.4mL, 3.2mL and 4.0mL into test tubes, and then Add 4mL, 3.2mL, 2.4mL, 1.6mL, 0.8mL and 0mL of distilled water to prepare the concentration of 0mg / mL, 0.2mg / mL, 0.4mg / mL, 0.6mg / mL, 0.8mg / mL and 1mg / mL Ascorbic acid solution.
[0031] Preparation of pentadecyl ferulic acid sample (named S6) solution: Take 10.0mg S 6 The sample (that is, the compound of formula (I) obtained in Example 1) was dissolved in 10 mL of distilled water to prepare a 1 mg / mL solution, and then pipette 0 mL, 0.8 mL, 1.6 mL, 2.4 mL, 3.2 mL and 4.0 mL into In the test tube, add distilled water 4.0mL, 3.2mL, 2.4mL, 1.6mL, 0.8mL and 0mL in sequence to make the concentration 0mg / mL, 0.2mg / mL, 0.4mg / mL, 0.6mg / mL, 0.8mg / ...
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