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New coumarin active content, preparation method and application thereof

A technology for coumarins and active ingredients, which is applied to a coumarin-based active ingredient and the field of preparation and application thereof, can solve the problems that the research on compounds, active ingredients, and mechanism of action is not clear enough, and further applications are affected.

Active Publication Date: 2011-02-16
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, pigeonpea leaves have been used more and more widely as a Chinese herbal medicine, but the research on active ingredients and mechanism of action is not clear enough, which affects its further clinical application
In the reports on the components of pigeonpea leaves, there has not been any report on compounds with structures such as pigeonholactone

Method used

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  • New coumarin active content, preparation method and application thereof
  • New coumarin active content, preparation method and application thereof
  • New coumarin active content, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. Separation of Coumarin Active Compounds in Pigeon Leaf

[0038] Take by weighing 5kg of pigeon pea dry branches and leaves, with 80% supercritical CO 2 Extract 3 times, 2 hours each time. After the extract is concentrated, add it to warm water (4L) at 40°C, vibrate and flocculate under the condition of 50KHz in an ultrasonic extractor for 30 minutes, rest for 30 minutes, take the floc, add quartz sand equal to 2 times the volume, stir and put it into a glass column, and then Add 8 / 1 (v / v) chloroform-water with 12mL / g sample volume, feed air at a flow rate of 0.25L / min for 30min, after static separation, recover the organic solvent to obtain the crude product of the target Dissolve a small amount of methanol, add an equal mass of chromatographic silica gel with a mesh number of 300-400, mix well, and evaporate to dryness under reduced pressure at 40°C. Weigh 300-400 mesh silica gel 4 times the weight of the extract in advance, use petroleum ether to wet-pack...

Embodiment 2

[0039] Example 2.DPPH free radical scavenging activity test

[0040] Add 100 μl of the sample solution to be tested and 3.5ml of DPPH solution into the test tube, keep it in a constant temperature water bath at 30°C for 20 minutes, measure its absorbance at 520 nm after taking it out, and use a blank solvent as a control. 5 concentrations were made for each sample, and 3 parallels were made for each concentration, and antibic acid was used as a positive control. Statistical processing of experimental results Calculation of IC of free radical scavenging activity 50 value.

Embodiment 3

[0041] Example 3. β-carotene-linoleic acid antioxidant activity test

[0042] Add 100μl of the sample solution to be tested and 4.9ml of β-carotene-linoleic acid emulsion into the test tube, use the solvent as a blank control, measure its absorbance at 470nm, which is the value at 0min, prepare a constant temperature water bath at 50°C, and measure well Absorbance at 0 min. Immediately pour the test solution back into the test tube and put it in a water bath, and measure it every 15 min until the β-carotene in the blank control tube fades. BHT solution is used as a positive control. 5 concentrations were made for each sample, and 4 parallels were made for each concentration. Calculation of antioxidant activity IC 50 value.

[0043] Table 2 is the results of antioxidant activity in vitro.

[0044] Table 2 Antioxidant and oxidation activity IC of pigeonilactone 50

[0045]

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PUM

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Abstract

The invention relates to a new coumarin active content, i.e. pigeon pea lactone extracted and separated from cajan leaves, an extraction and separation method and an application thereof. The structure of the compound is shown as a figure 1. The invention adopts the technical scheme that agricultural waste of cajan leaves is used as raw material which is extracted by a solvent; the extracting solution is concentrated and dispersed in water; an ultrasonic oscillation flocculation technology, a bubble column extraction technology and a column chromatography separation technology in positive-phase silica gel are adopted and combined with a low-temperature crystallization and recrystallization technology to obtain the content with the purity higher than 95 percent; and the anti-oxidation activity and the action of the chemical content on cardiovascular diseases are detected by a DPPH model, a beta-renieratene-linoleic acid model, an in-texture SOD enzyme model, a platelet aggregation modelinduced by ADP in corpora and an aorta systole model induced by phenylephrine. Results show that the active content has favorable free radical scavenging activity, anti-oxidation activity in corpora and in vitro, platelet aggregation restraining action in corpora and a certain hemangiectasis action. Meanwhile, the preparation method of the pigeon pea lactone in the invention is easy and practicaland can obtain a product with high purity high yield and high added value in a short period which is suitable for industrialized production and application, can be further developed and researched into a new antioxidant as an additive for medicines, foods and cosmetics.

Description

technical field [0001] The invention relates to extracting and isolating a biologically active compound from traditional Chinese medicine, in particular to a biologically active lactone compound extracted and isolated from the leaves of the traditional Chinese medicine pigeon pea [Cajanuscajan L. Millsp.] and an extraction method thereof. The compound is phyllolide, chemical name: 7-hydroxy-5-O-methyl-8-(3-methyl-2-butene)-4-phenyl-9,10-dihydro-benzene And pyran-2-one. Background technique [0002] With the development of social productivity, people are increasingly pursuing health and longevity. Finding new active ingredients from natural products is one of the important ways to develop new drugs to treat clinical chronic diseases that endanger human health and reduce the occurrence of drug-induced diseases. The present invention uses modern extraction, separation and purification means, combined with advanced physical means and chemical methods, to study the active compon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/16A61K31/37A61P9/00A61P17/18A61P17/16A61P39/06A61P25/28A61P9/10A61P9/12A61P7/02A61Q17/04A61Q19/08A23L1/30A61K36/48A61K127/00A23L33/105
CPCY02P20/54
Inventor 付玉杰祖元刚孔羽吴楠刘威祖柏实张琳顾成波李庆勇罗猛
Owner NORTHEAST FORESTRY UNIVERSITY
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