Method for Simultaneously Separating and Purifying Multiple Flavonoids from Meconopsis whole-leaf Meconopsis

A technology of simultaneous separation of Meconopsis entire margin, applied in chemical instruments and methods, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problem of undiscovered chemical components of Meconopsis entire margin , to achieve the effect of fast separation speed

Inactive Publication Date: 2018-04-03
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is recorded in Tibetan medical books that Meconopsis whole-leaves uses the flowers as medicine, but no literature has been found to study the chemical constituents of Meconopsis whole-leaf flowers.
At present, there is no study on the separation and purification of flavonoids in Meconopsis lentilis by HSCCC method

Method used

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  • Method for Simultaneously Separating and Purifying Multiple Flavonoids from Meconopsis whole-leaf Meconopsis
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  • Method for Simultaneously Separating and Purifying Multiple Flavonoids from Meconopsis whole-leaf Meconopsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of 4 kinds of flavonoid components

[0032] (1) Preparation of crude extract

[0033] Weigh 10g of Meconopsis whole-leaf, pulverize with a powder machine, the powder is not larger than 0.3mm (basically all pass through No. 3 sieve), add 70% ethanol (v / v) 200mL, ultrasonic (45kHz, 300w) extraction 3 times, The filtrates were combined for 45 minutes each time, and the ethanol was recovered under reduced pressure to obtain a concentrated solution. The concentrated solution was placed in a -80°C refrigerator overnight, and then freeze-dried to obtain 3.11 g of dry extract, with a dry extract yield of 31.1%.

[0034] Take about 150mg of the extract, dissolve it in 50mL of water, transfer it to a separatory funnel, extract it with ethyl acetate and n-butanol in sequence, combine the ethyl acetate and n-butanol extracts, recover the solvent under reduced pressure at 45°C, and obtain the crude extract for later use .

[0035] (2) Separation and purifi...

Embodiment 2

[0055] Example 2 Preparation of 2 known flavonoid components

[0056] (1) Preparation of crude extract

[0057] Weigh 10g of Meconopsis whole-leaf, pulverize with a powder machine, the powder is not larger than 0.3mm (basically all pass through No. 3 sieve), add 70% ethanol (v / v) 200mL, ultrasonic (45kHz, 300w) extraction 3 times, The filtrates were combined for 45 minutes each time, and the ethanol was recovered under reduced pressure to obtain a concentrated solution. The concentrated solution was placed in a -80°C refrigerator overnight, and then freeze-dried to obtain 3.11 g of dry extract, with a dry extract yield of 31.1%.

[0058] Take about 150mg of the extract, dissolve it in 50mL of water, transfer it to a separatory funnel, extract it with ethyl acetate and n-butanol in sequence, combine the ethyl acetate and n-butanol extracts, recover the solvent under reduced pressure at 45°C, and obtain the crude extract for later use .

[0059] (2) Separation and purification...

Embodiment 3

[0066] Example 3 Research on Antioxidant Activity of Compounds of the Present Invention

[0067] 1 material

[0068] General Analysis TU-1950 UV Spectrophotometer (Beijing General Analysis Company); METTLER AE240 Electronic Balance (Mettler-Toledo (Shanghai) Co., Ltd.), KQ-250B Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.) .

[0069] DPPH (Tixiai (Shanghai) Chemical Industry Development Co., Ltd.); ascorbic acid (Guangdong Guanghua Science and Technology Co., Ltd.); BHT (Chengdu Kelong Chemical Reagent Factory), other reagents were of analytical grade, and water was distilled water. Experimental sample: compound 1quercetin-3-O-β-D-glucopyrannosy-(1→6)-β-D-glucopyranoside, compound 2quercetin3-O-[2”'-O-acetyl-β-D-glucopyranosyl-(1 →6)-β-D-glucopyranoside, compound 3quercetin 3-O-[6"'-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside, compound 4quercetin 3-O-[ 3"'-O-acetyl-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside. The above experimental samples were sep...

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Abstract

The invention provides a method for simultaneously separating and purifying four flavonoid components from Meconopsis whole-leaf flowers. The invention also provides a method for simultaneously separating and purifying two known flavonoid components. The present invention successfully separates and purifies four kinds of flavonoids from Meconopsis whole leaf by HSCCC method, and the separation speed is fast, which lays a foundation for the research on the material basis of Meconopsis whole leaf. It has been found through research that the above-mentioned isolated known compounds or new compounds have good free radical scavenging activities, and can be used as anti-oxidation drugs or health products.

Description

technical field [0001] The invention relates to a method for simultaneously separating and purifying multiple flavonoid components from Meconopsis whole margin leaves. Background technique [0002] Meconopsis integrifolia (Maxim.) Franch. is a plant of the genus Meconopsis in the family Papaveraceae. It is a traditional Tibetan medicinal material. It is mainly distributed in Tibet, Sichuan, Yunnan, Qinghai, Gansu and other provinces in my country. Area. "Jingzhu Materia Medica" records that Meconopsis whole-leaf is used as medicine to treat hepatitis, pneumonia and edema [1-2] . Modern chemical composition research has systematically studied the chemical composition of the above-ground parts of Meconopsis entire margin, using traditional separation methods to separate compounds such as alkaloids and flavonoids [3-5] . However, Tibetan medical books record that Meconopsis lentilis uses flowers as medicine, but no literature reports have been found on the chemical constitue...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P39/06
Inventor 刘圆黄艳菲韩亚涛李伯超
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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