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Preparation method of Hainan green plum stem extractive with function of scavenging free radicals

A stem extract and free radical technology, applied in the field of extraction and separation of plant active components, can solve problems such as chemical active components of Hainan green plum stem extract, etc., and achieve excellent free radical scavenging activity, clear chemical composition, and technological process. simple effect

Inactive Publication Date: 2014-08-06
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the chemically active components of Hainan green plum stem extract at home and abroad.

Method used

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  • Preparation method of Hainan green plum stem extractive with function of scavenging free radicals
  • Preparation method of Hainan green plum stem extractive with function of scavenging free radicals

Examples

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preparation example Construction

[0028] The preparation method steps of Hainan green plum stem extract are as follows:

[0029] 1) Take the stem of Hainan green plum, dry it in the shade at room temperature, crush it, pass through a 20-80 mesh sieve, then add methanol, acetone or 50-95% ethanol, soak at room temperature, heat or ultrasonically extract, and the extraction time is 60 minutes to 7 days , the ratio of solid to liquid is 1:1~1:10;

[0030] 2), filter the extract, recover under reduced pressure to obtain the total extract, add water to suspend, sequentially extract with petroleum ether and ethyl acetate, recover under reduced pressure to obtain the petroleum ether part and ethyl acetate part respectively; the ethyl acetate part is treated with chloroform Mixed solvent system with methanol (gradients are 100:0, 100:5, 100:30, 100:40, 100:60 and 100:100, volume ratio) for gradient elution, and thin-layer chromatography detects and merges the same components to obtain 6 components A-F (A, 100:0; B, 100...

example 1

[0034] Example 1 The preparation of the effective part of Hainan green plum stem

[0035] Hainan green plum stems (10Kg), dried in the shade at room temperature, pulverized, soaked at room temperature with 75% ethanol for 3 times, 7 days / time, combined the extracts, concentrated under reduced pressure to obtain the total extract (1.0Kg), added water to suspend and disperse, and used Petroleum ether and ethyl acetate were extracted to obtain a petroleum ether part (50 g) and an ethyl acetate part (400 g).

[0036] The ethyl acetate part was separated by silica gel column chromatography, and chloroform:methanol (100:0, 100:5, 100:30, 100:40, 100:60 and 100:100, volume ratio) was used for gradient elution, and the chloroform : Methanol (100:30) eluted the obtained components through repeated silica gel column chromatography and Sephdex LH-20 column chromatography to obtain 16 resveratrol oligomer compounds, respectively For repeated chloroform: ethyl acetate (1:2) silica gel col...

Embodiment 2

[0038] Example 2 Study on the activity of scavenging DPPH free radicals by the polar components of the ethyl acetate extraction parts of Hainan green plum stems

[0039] 1. Preparation of test solution

[0040] 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) was formulated into a 65 μM absolute ethanol solution. Precisely weigh the test samples and dissolve them in absolute ethanol for later use.

[0041] 2. Experimental method

[0042] Pipette 50 μL of the test sample and add it to 3 mL of 65 μM DPPH solution, and use an equal amount of absolute ethanol for the blank control, mix well, place it at room temperature for 30 minutes, then place it in a cuvette, and measure the absorbance of the mixed solution of the sample and DPPH at 517 nm. Each absorbance was measured 3 times in parallel, and the average value was taken to calculate the clearance rate of the series of concentrations to obtain the IC 50 value. The clearance rate (Y) is calculated according to the following fo...

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Abstract

The invention discloses a Hainan green plum stem extractive with a function of scavenging free radicals and a preparation method thereof. The preparation method comprises the following steps: adding an organic solvent to Hainan green plum stem drying powder, and extracting; filtering an extracting solution, concentrating the filtered extracting solution under reduced pressure so as to obtain total extract, adding water, suspending, extracting sequentially by using petroleum ether and ethyl acetate, recovering under reduced pressure so as to respectively obtain a petroleum ether part and an ethyl acetate part, carrying out gradient elution on the obtained ethyl acetate part by using a chloroform and methyl alcohol mixed solvent system, wherein the chloroform and methyl alcohol component with the volume ratio of chloroform to methyl alcohol being 100: 30 has excellent free radical scavenging activity and comprises 16 resveratrol oligomers compounds. The Hainan green plum stem extractive prepared by the method is high in yield, simple in technological process, low in cost and suitable for large-scale preparation; and the obtained extractive has excellent free radical scavenging activity and can be applied to healthcare food, medicines and food with functions of resisting oxidation and scavenging free radicals.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of plant active components, and relates to a method for extracting and separating plant active components and their active ingredients, in particular to a preparation method of Hainan green plum stem extract with the function of scavenging free radicals. Background technique [0002] Vatica is the largest subgenus in Dipterocarpaceae, with about 65 species. It is mainly distributed in tropical Asia and is a dominant tree species in tropical rain forests. There are three kinds of green plums in my country, Hainan green plums, namely green plums (V. mangachapoi Blanco ssp. hainanensis), Guangxi green plums (V. guangxiensis X.L.) and Banna green plums (V. xishuangbannaensis G.D.). Among them, the world distribution of Hainan green plum is mainly in Vietnam, Thailand, the Philippines, Indonesia and other places. In my country, Hainan green plum is only distributed in Hainan Province....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A23L1/29A61P39/06A61K135/00A23L33/00
Inventor 陈光英韩长日陈文豪宋小平惠阳
Owner HAINAN NORMAL UNIV
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