A kind of preparation method and application of kunlun chalcone chalcone compound

A compound and technology, applied in the field of kunlun chalcone chalcone compound and its preparation, can solve the problems of underestimating the total active ingredient content and not considering the binding compound, etc., and achieve the effect of strong free radical scavenging activity

Active Publication Date: 2016-05-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, existing studies on the phytochemical constituents of snow chrysanthemum underestimated the content of the total active ingredients by not taking into account the conjugated compounds

Method used

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  • A kind of preparation method and application of kunlun chalcone chalcone compound
  • A kind of preparation method and application of kunlun chalcone chalcone compound
  • A kind of preparation method and application of kunlun chalcone chalcone compound

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 compound

[0025] Weigh 5 kg of dried Kunlun snow chrysanthemum flower heads, mash it with a homogenizer mixer, put it in a 20L volumetric flask, add 8-15L methanol, and repeat the extraction three times. Use a Buchner funnel to suction filter, place the filter residue in a 20L volumetric flask, and use 2mol·L -1 The NaOH solution was degraded in a water bath at 50°C for 2 hours, neutralized to neutral with hydrochloric acid, then extracted by adding 3-5 times (volume ratio) n-butanol, left for 24 hours, and extracted three times. Use a rotary evaporator to concentrate in vacuo at 55°C to obtain n-butanol extract.

[0026] The n-butanol extract (152 g) was mixed with 100 g of silica gel in a dry method, then mounted on an atmospheric column equipped with 300 g of silica gel (300-400 mesh), and separated by petroleum ether-acetone-methanol gradient elution. The eluted components were concentrated in vacuum and then detected by TLC. The T...

Embodiment 2

[0033] The test of embodiment 2 antioxidant activity

[0034] 1. Experimental samples and experimental methods

[0035] 1.1 DPPH method.

[0036] Preparation of DPPH solution: Accurately weigh 0.0197g DPPH, and adjust the volume to 250ml with absolute ethanol to obtain a DPPH solution with a concentration of 2×10-4mol·L-1.

[0037] Measuring method: add 2ml DPPH solution to 2ml sample, shake well and keep it in the dark for 30min, measure the absorbance value AI at 517nm, replace the blank with 2ml absolute ethanol; add 2ml absolute ethanol to the 2ml sample, shake it well and keep it in the dark 30min, the absorbance value AJ was measured at 517nm; add 2ml DPPH solution to 2ml of absolute ethanol, shake well, and then stand in the dark for 30min, and the absorbance value AC was measured at 517nm; the clearance rate was [1-(AI-AJ) / AC ] × 100%. VE solution (20-100 μmol·L-1) was used as a positive control, and each sample was parallelized three times.

[0038] 1.6.3 ABTS met...

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Abstract

The invention discloses a Kunlun chrysanthemum chalcone compound as well as a preparation method and application thereof. A structural formula of the compound is as shown in a formula (I). Alpha,3,2'-trihydroxy-4'-O-beta-D-glucopyranose chalcone separated from Kunlun chrysanthemum is a compound which is obtained from a natural resource for the first time; the compound has high oxidative activity and very high radical-scavenging activity, and can be used as a representative anti-oxidative active ingredient and a natural radical-scavenging agent in the Kunlun chrysanthemum. The formula (I) is as shown in the specification.

Description

technical field [0001] The invention belongs to the field of extraction and separation of natural compounds, and in particular relates to a chalcone compound of chamomile chalcone and its preparation method and application. Background technique [0002] Epidemiological studies continue to show that oxidative stress caused by free radical imbalance is the main cause of various cardiovascular diseases in the human body, such as hypertension, hyperlipidemia and cancer. Therefore, the human body supplements a certain amount of antioxidants to remove Excess free radicals are very necessary to maintain human health. Synthetic antioxidants commonly used in the food industry, such as di-tert-butyl-p-cresol and tert-butylhydroquinone, have significant antioxidant effects, but the amount of use is strictly limited, and excessive use often has certain side effects. Therefore, searching for natural antioxidants with high efficiency and no side effects from various plants, in order to p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08A61P39/06A61Q19/00A23L33/105A23L33/125
Inventor 辛志宏陈伟冉火苗张俊楠王伟郭佳
Owner NANJING AGRICULTURAL UNIVERSITY
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