Polysiphonia urceolata bromophenol compound as well as preparation method and application thereof

A polytubular algae and compound technology, applied in the field of food additives, can solve the problems of reducing food nutritional value, affecting food quality, food fading, etc., achieving significant DPPH free radical scavenging activity, improving product quality, and extending shelf life.

Inactive Publication Date: 2008-05-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only that, the oxidation reaction can also lead to food fading, browning, etc., which seriously affects the quality of food and reduces the nutritional value of food

Method used

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  • Polysiphonia urceolata bromophenol compound as well as preparation method and application thereof
  • Polysiphonia urceolata bromophenol compound as well as preparation method and application thereof
  • Polysiphonia urceolata bromophenol compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of bromophenol compound:

[0024] Collect fresh Polytubule algae and wash them with seawater to remove the sand and algae attached to the surface, and dry them in a cool place for later use. Take 6.1Kg of the dried sample, crush it to 60-80 mesh, extract it three times with 8.5 liters of 95% ethanol at room temperature, three days each time, combine the ethanol extract, concentrate under reduced pressure at a temperature of about 40°C, and recover ethanol , to obtain ethanol crude extract. Add distilled water twice the volume of the obtained crude extract to the crude extract to suspend it in water, and then extract three times each with petroleum ether, ethyl acetate, and n-butanol that are twice the volume of the distilled water of the above-mentioned suspended crude extract. , were concentrated to obtain the corresponding petroleum ether, ethyl acetate, n-butanol extracts. The ethyl acetate extract (60 g) was subjected to silica gel column ch...

Embodiment 2

[0031] The difference from Example 1 is:

[0032] The dried and pulverized Polytubella was extracted with 80% ethanol four times, each time for three days, the extracts were combined, concentrated under reduced pressure at room temperature to obtain a crude extract, and the ethanol was recovered at the same time. Suspend the crude extract in water with 3 times the volume of the crude extract, extract three times each with petroleum ether, ethyl acetate, and n-butanol that are 2 times the volume of the distilled water of the above-mentioned suspended crude extract, and respectively concentrate to obtain the corresponding Petroleum ether, ethyl acetate, n-butanol extract. The ethyl acetate extract was subjected to silica gel column (220 g, 200-300 mesh) chromatography, and gradient elution was performed with ethyl acetate-methanol with a gradient of 100:0 to 0:100, and the eluents were collected respectively. Detect with thin-layer chromatography (TLC) (using ferric chloride as...

Embodiment 3

[0041] DPPH free radical scavenging activity test:

[0042] DPPH is a stable free radical, which is purple in organic solvents and has a strong absorption near 517nm. After adding antioxidants, some free radicals are removed, which weakens the absorption, which can be used to evaluate its antioxidant activity and free radicals. The strength of scavenging activity. This method is easy to operate and has good reproducibility.

[0043] experimental method:

[0044] Add 2mL DPPH (methanol solution, 0.16mmol / L) to 2mL of the sample obtained in Example 1 and mix evenly. After placing it in a dark place at room temperature for 50min, measure its absorbance A at 517nm. sample .

[0045] Add 2mL methanol solution to measure its absorbance A at 517nm in the sample obtained in 2mL Example 1 sample blank . Add 2mL methanol chloroform mixed solvent to 2mLDPPH and measure its absorbance A at 517nm control . Add 2mL methanol chloroform mixed solvent to 2mL methanol and measure its abs...

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Abstract

The invention relates to the technical field of food additives, in particular to a bromphenol compound in polysiphonia urceolata and the preparation method and purpose. The invention is characterizedin that the bromphenol compound capable of eliminating the activity of free radical can be obtained from the polysiphonia urceolata of oceanic red alga by the method of extraction and separation, theconstitutional formula (I) or (II) is shown, wherein the R1 is H, Br, OH, OCH3 or OCH2CH3; the R2 is H, Br, OH, OCH3 or OCH2CH3; the R3is H, Br, OH, OCH3 or OCH2CH3, the preparation method is characterized in that the polysiphonia urceolata is washed by seawater; the gravels and miscellaneous algae on the surface of the polysiphonia urceolata are removed, and then the leaching, solvent distribution and repeated chromatographic separation are implemented after the polysiphonia urceolata is dried in the shade and crushed, so that a monomer compound is obtained, wherein the R1 is H, Br, OH, OCH3or OCH2CH3; the R2 is H, Br, OH, OCH3 or OCH2CH3; the R3is H, Br, OH, OCH3 or OCH2CH3. The bromphenol compound which is obtained by the preparation method of the invention has the advantages that: thebromphenol compound can be used as the food additives to delay or prevent the oxidation and metamorphosis of edible oil or oil-retaining food because of the inducement of the free radical.

Description

technical field [0001] The invention relates to a food additive, in particular to a bromophenol compound in the marine red alga Polytubella and its preparation method and application. Background technique [0002] Oxidation reactions induced by free radicals can lead to rancidity of foods, especially oily foods. Not only that, the oxidation reaction can also lead to food fading, browning, etc., which seriously affects the quality of food and reduces the nutritional value of food. On the other hand, many oxidation products and intermediate products caused by free radicals are recognized carcinogens, which can seriously damage the organism's biofilm, protein, enzyme and cell function, and directly or indirectly cause many diseases such as cancer, atherosclerosis, diabetes, etc. , neurodegenerative diseases, and aging of the body. [0003] One of the main ways to prevent the oxidative deterioration of edible oils and oily foods commonly used at present is to add antioxidants ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/42C07C37/68A23L1/03A23L3/349A23D7/06A23D9/06A23L29/00
Inventor 王斌贵李可李晓明
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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