Method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials

A determination method and technology of clavus, applied in the field of content determination of ellagic acid components in clavus medicinal materials, can solve problems such as less research on clavus medicinal materials, weak basic research, lack of qualitative and quantitative method reference substances, etc.

Active Publication Date: 2010-07-28
贵州益康制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the literature survey finds that there are few studies on clavus medicinal materials, weak basic research, lack of reliable qualitative and quantitative methods and available contrast substances, and only its simple thin layer identification
Therefore, the preparation Xuemaitong Capsules developed with clavus as the main drug has relatively simple quality control standards, especially the detection of content determination and other items for the main drug of the prescription, clavus, which cannot well ensure the effectiveness of its clinical medication. and safety, further research is needed to improve its quality control standards

Method used

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  • Method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials
  • Method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials
  • Method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0128] Embodiment 1 of the present invention: the content determination method of ellagic acid composition in clavus medicinal material is:

[0129] Adopt ultra-high performance liquid chromatography, specifically comprise the following steps:

[0130] (1) Chromatographic conditions and system suitability test:

[0131] Instrument: Waters ACQUITY UPLC chromatograph (including binary gradient pump, vacuum degasser, autosampler, column oven, diode array detector, Epower 2 workstation);

[0132] Chromatographic column: ACQUITY UPLC BEH C18 column (2.1mm×100mm, 1.7μm);

[0133] Mobile phase: A is acetonitrile, B is 0.1% H 3 PO4, carry out gradient elution according to A:B=6~100:94~0; during the gradient elution process of the mobile phase, the phase A and phase B of the eluent change as follows: at the beginning, phase A is 6%, phase B is 94% , stable for 1 minute; at the 19th minute, phase A was 35%, and phase B was 65%; at the 22nd minute, phase A was 100%, and phase B was 0;...

Embodiment 2

[0144] Embodiment 2 of the present invention: the method for determining the content of ellagic acid components in clavus medicinal materials can also be:

[0145] Adopt ultra-high performance liquid chromatography, specifically comprise the following steps:

[0146] (1) Chromatographic conditions and system suitability test:

[0147] Instrument: Waters ACQUITY UPLC chromatograph;

[0148] Chromatographic column: ACQUITY UPLC BEH C18 column;

[0149] Mobile phase: A is acetonitrile, B is 1% H 3 PO4, carry out gradient elution according to A:B=6~100:94~0; during the gradient elution process of the mobile phase, the phase A and phase B of the eluent change as follows: at the beginning, phase A is 6%, phase B is 94% , stable for 1 minute; at 19 minutes, phase A is 35%, and phase B is 65%; at 22 minutes, phase A is 100%, and phase B is 0.

[0150] Flow rate: 0.2mL / min;

[0151] Column temperature: 35°C;

[0152] Detection wavelength: 235~250nm;

[0153] Injection volume: 1μ...

Embodiment 3

[0158] Embodiment 3 of the present invention: the method for determining the content of ellagic acid components in clavus medicinal materials can also be:

[0159] Adopt ultra-high performance liquid chromatography, specifically comprise the following steps:

[0160] (1) Chromatographic conditions and system suitability test:

[0161] Instrument: Waters ACQUITY UPLC chromatograph;

[0162] Chromatographic column: ACQUITY UPLC BEH C18 column;

[0163] Mobile phase: A is acetonitrile, B is 0.5% H3PO4, gradient elution is carried out according to A:B=6~100:94~0; during the gradient elution process of the mobile phase, the phase changes of A and B of the eluent are: start At 22 minutes, phase A is 100% and phase B is 0.

[0164] Flow rate: 0.2mL / min;

[0165] Column temperature: 45°C;

[0166] Detection wavelength: 240~260nm;

[0167] Injection volume: 1μL;

[0168] The number of theoretical plates is calculated to be above 160,000.

[0169] (2) Preparation of reference solu...

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Abstract

The invention provides a method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials, which synchronously measures the contents of at least two of five ingredients of ellagic acid, 3,3'-dimethoxy ellagic acid, 3,3'-dimethoxy ellagic acid-4'-O-beta-D-xyloside, 3,3'-dimethoxy ellagic acid-4'-O-beta-D-glucoside and 3,3'-dimethoxy ellagic acid-4'-O-alpha-D-arabinoside contained in the euscaphis japonica medicinal materials by using a high efficiency liquid chromatography or an ultra-high efficiency liquid chromatography under the same chromatogram condition. In the invention, the contents of the multiple ingredients in the euscaphis japonica medicinal materials are measured under the same chromatogram condition so that the method for measuring the content of the ellagic acid ingredient in the euscaphis japonica medicinal materials has better integrity, characteristics and stability as well as high precision, favorable repeatability and high recovery rate and capability of effectively controlling the quality of the euscaphis japonica medicinal materials, thereby ensuring the effectiveness and the safety of clinical medication and laying the foundation for redevelopment and application of the euscaphis japonica medicinal materials.

Description

Technical field: [0001] The invention relates to a content determination method of ellagic acid components in clavus medicinal materials, belonging to the technical field of quantitative detection of traditional Chinese medicinal materials. Background technique: [0002] Chicken eyes are the branches and leaves of Euscaphis japonica (Thunb.) Dippel with flowers or fruits of the oil family plant Euscaphis japonica (Thunb.) Dippel. Its original plants are distributed in various provinces in the south of the Yangtze River and northeastern Yunnan. (2003 edition), "Sichuan Traditional Chinese Medicine Annals" uses its fruit or seeds. Corn eyes are used by ethnic minorities in Guizhou. It is pungent, sweet, and flat in nature. 2002 (Trial) One of the main medicinal materials in Xuemaitong capsules. [0003] Xuemaitong Capsule (WS-10108(ZD-0108)-2002) has the functions of promoting blood circulation and removing blood stasis, dredging meridians and activating collaterals. It is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/36A61K36/185
Inventor 王爱民张秋生高翔刘智兰燕宇李勇军
Owner 贵州益康制药有限公司
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