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Preparation method for obtaining uniform xylan from corn cob

A technology of xylan and corn cob, which is applied to the preparation of sugar derivatives, oligosaccharides, chemical instruments and methods, etc., and can solve problems such as no patents yet

Inactive Publication Date: 2013-01-23
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method for obtaining uniform xylan from corn cob
  • Preparation method for obtaining uniform xylan from corn cob
  • Preparation method for obtaining uniform xylan from corn cob

Examples

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Embodiment 1

[0020] (1) Select fresh, moth-eaten, and mildew-free corn cobs, and after drying, smash them into small pieces with a hammer, dry them, and put them in a grinder to crush them. The crushed corn cob powder is passed through a 20-mesh sieve for subsequent use.

[0021] (2) Weigh 80 g of corncob powder (larger particles), add distilled water at a solid-to-liquid ratio of 1:8, heat-treat in a water bath at 90°C for 1 hour, and filter out the liquid with nylon gauze.

[0022] (3) Add 1200mL 10g / 100mL NaOH solution to the remaining solid, stir and extract at 80°C for 2h, after the solution is cooled, adjust the pH to 7.7 with HCL, centrifuge at 8000rpm / min for 10min, and obtain the precipitate and supernatant respectively.

[0023] (4) The supernatant was concentrated by rotary evaporation, and 95% ethanol was added to make the final concentration 80%, and alcohol precipitation was carried out overnight. Centrifuge at 8000rpm / min for 10min to collect the precipitate, and then redis...

Embodiment 2

[0029] (1) Select fresh, moth-eaten, and mildew-free corn cobs, and after drying, smash them into small pieces with a hammer, dry them, and then place them in a grinder for crushing. The crushed corn cob powder is passed through a 20-mesh sieve for subsequent use.

[0030] (2) Weigh 20 g of corncob powder, add distilled water at a solid-to-liquid ratio of 1:6, heat-treat in a water bath at 80° C. for 1 hour, and filter out the liquid with nylon gauze.

[0031] (3) Add 300mL 10g / 100mL NaOH solution to the remaining solid, stir and extract at 80°C for 2h, after the solution is cooled, filter it with nylon gauze, adjust the pH of the filtrate to 5.8 with anhydrous acetic acid, add 95% ethanol, the final concentration is 50%, alcohol precipitation overnight. Centrifuge at 8000rpm / min for 10min to collect the precipitate. After rehydration (solid-to-liquid ratio 1:15, 60°C, 2h), centrifuge at 8000rpm / min for 10min to obtain supernatant A and precipitate B.

[0032] (4) Precipitat...

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Abstract

The invention relates to the field of the preparation of xylan, and particularly relates to a method for preparing and purifying uniform xylan from a corn cob. A framework is formed by xylose repeating units through the connection of beta-D-1,4 xyloside bonds, and xylose residues account for 99% or above. In addition to xylose, the xylan prepared in the prior art also has other monosaccharide accounting for a certain percentage, such as galactose, glucose, arabinose, glucose and some uronic acids. According to the method, 99% (or above) of monosaccharide components of the xylan prepared through a special process are xylose. The method comprises the following specific steps: crushing the corn cob; sieving with a 20-mesh sieve; and performing alkaline extraction at high temperature, wherein the solid-to-liquid ratio is (1:2)-(1:20), the concentration of an alkaline solution is 2-20%, and the reaction time is 1-6 hours. According to the invention, the main chain of the prepared xylan is composed of 2-3 hundreds of xylose residues; and through the connection of beta-1,4 glycosidic bonds, the problem that other miscellaneous monosaccharide is contained when xylo-oligosaccharide is obtained through the degradation of xylan is well solved. Thus, the invention has good application prospects.

Description

1. Technical field [0001] The invention relates to the field of xylan preparation, in particular to a preparation method for obtaining homogeneous xylan from corn cobs. In particular, it relates to a method for preparing homogeneous xylan with simple process and suitable for large-scale industrial production. 2. Background technology [0002] At present, the xylans produced by general methods are heterogeneous xylans with branched chain structures. No matter whether it is produced by enzymatic hydrolysis or high temperature degradation, xylo-oligosaccharides contain other Sugar, under the current technical conditions, it is difficult to remove other monosaccharides except xylose, so the cost of producing real xylooligosaccharides is very high. The homogeneous xylan produced by the invention can be directly degraded to produce xylooligosaccharides, xylose and xylitol, and hardly contains other monosaccharides, and the purity is over 99%. It has a good prospect of commercial...

Claims

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Application Information

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IPC IPC(8): C08B37/14C07H3/06C07H1/08
Inventor 尹鸿萍王莹屈清慧李明皓陈书军倪孟祥
Owner CHINA PHARM UNIV
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