Viili extracellular polysaccharide and preparation method thereof
A technology of exopolysaccharide and polysaccharide, applied in the field of deep processing of dairy products, can solve the problem of no patent for exopolysaccharide, and achieve the effect of high purity
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[0031] Below in conjunction with embodiment, the present invention will be further described, and following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.
[0032] The Viili exopolysaccharide of the present invention is obtained from commercial Viili inoculated fermented milk.
[0033] A preparation method of Viili exopolysaccharide, the steps are as follows:
[0034] (1) Fermentation: 500mL of fresh milk was sterilized at 115°C for a short time, then inserted into 10mL of Viili, placed in an incubator at 31°C, and incubated for 16 hours. After the incubation, it was taken out and placed in a refrigerator at 4°C for 8 hours;
[0035] (2) Casein removal by isoelectric point method: Take out the yogurt in the refrigerator, adjust the pH of the fermented milk to 8.0 with saturated NaOH solution, centrifuge at 4000r / min for 15min, take the supernatant, and adjust the pH of the supernatant to 4.6, centri...
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