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Viili extracellular polysaccharide and preparation method thereof

A technology of extracellular polysaccharides and polysaccharides, which is applied in the field of deep processing of dairy products, can solve the problems of no patents on extracellular polysaccharides, and achieve high purity

Inactive Publication Date: 2013-04-10
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, most of the patent reports on exopolysaccharides focus on fungal polysaccharides and some bacterial polysaccharides, and there is no patent on exopolysaccharides in Viili yoghurt

Method used

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  • Viili extracellular polysaccharide and preparation method thereof
  • Viili extracellular polysaccharide and preparation method thereof
  • Viili extracellular polysaccharide and preparation method thereof

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Embodiment Construction

[0031] Below in conjunction with embodiment, the present invention will be further described, and following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0032] The Viili exopolysaccharide of the present invention is obtained from commercial Viili inoculated fermented milk.

[0033] A preparation method of Viili exopolysaccharide, the steps are as follows:

[0034] (1) Fermentation: 500mL of fresh milk was sterilized at 115°C for a short time, then inserted into 10mL of Viili, placed in an incubator at 31°C, and incubated for 16 hours. After the incubation, it was taken out and placed in a refrigerator at 4°C for 8 hours;

[0035] (2) Casein removal by isoelectric point method: Take out the yogurt in the refrigerator, adjust the pH of the fermented milk to 8.0 with saturated NaOH solution, centrifuge at 4000r / min for 15min, take the supernatant, and adjust the pH of the supernatant to 4.6, centri...

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Abstract

The invention relates to a viili extracellular polysaccharide and a preparation method thereof. The extraction of the viili extracellular polysaccharide mainly comprises: enzymolysis, deproteinization, alcohol precipitation and other steps. The separation and purification of extracellular polysaccharide mainly comprises ion-exchange column chromatography and gel filtration chromatography. The invention also discloses the composition of the viili extracellular polysaccharide, which is that the viili extracellular polysaccharide mainly comprises rhamnose, L-arabinose, xylose, mannose, glucose and galactose in a molar ratio of 7.41:15:8.85:1:3.25:1.25. The monosaccharide residue of the viili extracellular polysaccharide exists in form of pyranoid ring and furan ring. The preparation method and product of the viili extracellular polysaccharide are suitable to be used as liposome nanometer materials and functional factors for functional foods and helps to develop some special medicine carrier materials and novel functional foods.

Description

technical field [0001] The invention belongs to the field of deep processing of dairy products, and relates to a method for extracting exopolysaccharides, in particular to a Viili exopolysaccharide and a preparation method thereof. Background technique [0002] Exopolysaccharides (Exopolysaccharides, EPS) are the mucus or capsular polysaccharides secreted by microorganisms outside the cell wall during growth and metabolism, which are easy to separate from the bacteria. Microbial polysaccharides can not only be used as gelling agents, preservatives, emulsifiers, film-forming agents and other additives in the food and pharmaceutical industries to change the viscosity and hardness of products, but also have many functions such as anti-oxidation, anti-aging, enhancing immunity and lowering cholesterol. function. Therefore, these polysaccharides have become an important source of substances with specific physiological activities, and the development and utilization of these exop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/08
Inventor 王昌禄韩晓梅陈勉华李风娟王玉荣
Owner TIANJIN UNIV OF SCI & TECH
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