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Method for extracting and separating vitexin xyloside from natural product

A technology of vitexin xyloside and natural product, applied in chemical instruments and methods, sugar derivatives, sugar derivatives and other directions, to achieve the effects of low cost, simple process and safe solvent

Active Publication Date: 2015-10-14
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although it has been reported in the literature that pineapple leaves contain flavonoids, so far, there has been no literature report that pineapple leaves contain vitexin xyloside, nor has any literature reported the extraction and separation of high-purity vitexin xyloside from pineapple leaves. Preparation

Method used

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  • Method for extracting and separating vitexin xyloside from natural product
  • Method for extracting and separating vitexin xyloside from natural product

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Take 1kg of wood pineapple leaves (tested by high performance liquid chromatography, the content of vitexin xyloside in this batch of wood pineapple leaves is 2.5mg / g, and 1 kg of wood pineapple leaves contain 2.5g of vitexin xyloside in total) and crushed into coarse powder, Add 15L of 30% ethanol for reflux extraction for 60 minutes, filter, add 10L of 30% ethanol for reflux extraction for 30 minutes, filter, combine the two extracts, recover ethanol under reduced pressure, and concentrate the extract to 0.5 L, the extract was allowed to stand at room temperature for 12 hours and filtered to obtain a concentrated solution. The concentrated solution was passed through a D101 macroporous adsorption resin column with a column volume of 5L for saturated adsorption, followed by elution with 20L of pure water and 20L of 10% ethanol to remove impurities, followed by 20L of 30% ethanol solution to elute the vitexin xyloside to remove 30% Ethanol eluate was recovered under redu...

Embodiment 2

[0027] Take 1kg of wood pineapple leaves (tested by high performance liquid chromatography, the content of vitexin xyloside in this batch of wood pineapple leaves is 2.5mg / g, and 1 kg of wood pineapple leaves contain 2.5g of vitexin xyloside in total) and crushed into coarse powder, Add 15L of 50% ethanol for ultrasonic extraction for 60 minutes, filter, add 10L of 50% ethanol for ultrasonic extraction for 30 minutes, filter, combine the two extracts, recover the ethanol under reduced pressure, and concentrate the extract to 0.8 L, the extract was allowed to stand at room temperature for 24 hours and filtered to obtain a concentrated solution. The concentrated solution was passed through an X-5 macroporous adsorption resin column with a column volume of 6.5L for saturated adsorption, followed by elution with 30L pure water and 30L 20% ethanol to remove impurities, and then 30L 50% ethanol solution to elute vitexin xyloside. The 50% ethanol eluent was decompressed to recover eth...

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Abstract

The invention relates to a method which is easy for industrial separation and purification of vitexin xyloside. The method has high extraction efficiency. The technology comprises steps as follows: extraction of ethanol, macroporous resin column chromatography and preparation of high performance liquid chromatography for separation and purification. Purity of vitexin xyloside prepared by the method reaches more than 90%. The preparation method is safe, simple and low-cost, and is suitable for industrial production.

Description

Technical field [0001] The invention relates to a method for extracting and separating vitexin xyloside from natural products, in particular to a method for extracting and separating vitexin xyloside from pineapple leaves. Background technique [0002] Vitexin xyloside or vitexin xyloside, its English name is vitexin-2″-O-xyloside or vitexin-2-O-xyloside, molecular formula C 26 H 28 O 14 , Molecular weight: 564, structural formula is as follows: [0003] [0004] Artocarpus heterophyllus lam (Artocarpus heterophyllus lam) is a plant belonging to the genus Mooraceae. Its fruit is also called jackfruit, tree pineapple, honey melon, cow belly fruit, etc. It is a world-famous tropical fruit native to tropical Asia and is widely cultivated in tropical humid regions It has been introduced into my country for more than 1,000 years. The flesh of the wood pineapple is flat, sweet and slightly sour, non-toxic, and has the effects of producing body fluid, quenching thirst and irritability, so...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 潘小姣邓家刚韦海红李耀华梁臣艳梁洁
Owner GUANGXI UNIV OF CHINESE MEDICINE
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