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Application of apple residue and apple leaf extract to prevention and control of phytopathogen

A technology of plant pathogenic bacteria and apple pomace, applied in the fields of application, plant growth regulator, botanical equipment and methods, etc., can solve the problems of resource loss, low probability of effective use, and odor, and achieve significant antibacterial activity and good control effect of effect

Inactive Publication Date: 2018-12-21
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In these processing processes, a large amount of by-products of apple pomace will be produced, about several million tons per year, but the probability of its effective use is very low, and a small amount will be used to extract pectin and as feed, fertilizer, fuel, Most of the others are directly treated as garbage, causing a huge loss and waste of resources. At the same time, because the discarded apple pomace contains a lot of water, if it cannot be disposed of in time, it is easy to breed a large number of microorganisms, and then rot, sour, and smelly , causing serious pollution to the environment and seriously affecting the sustainable development of the apple processing industry
However, there is no report on the use of apple pomace extracts to prevent and treat plant diseases.

Method used

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  • Application of apple residue and apple leaf extract to prevention and control of phytopathogen
  • Application of apple residue and apple leaf extract to prevention and control of phytopathogen
  • Application of apple residue and apple leaf extract to prevention and control of phytopathogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Embodiment 1: Apple pomace ethanol extract prevents and treats tomato gray mold and canker

[0009] Taking tomato Botrytis cinerea and canker as objects, the control effect of apple pomace ethanol extract on the two diseases was evaluated by artificial inoculation technique in potted plants. Test crops: tomato. Pathogens tested: tomato gray mold (Botrytis cinerea), tomato canker (Clavibacter michiganensis subsp. Michiganensis).

[0010] Experimental design: the concentration of ethanol extract of apple pomace was diluted 200 times, the control agent for tomato gray mold was 50% procymidone wettable powder, and the control agent for canker was 500 million spores / g Pseudomonas fluorescens wettable powder , 1 water control (only inoculated with bacteria), 1 blank control. Each treatment was replicated 4 times, and each replicated 10 seedlings.

[0011] Test method: Spraying is used to apply pesticides, and after spraying treatment, pathogenic bacteria are artificially i...

Embodiment 2

[0016] Embodiment 2: The in vitro activity of apple pomace ethanol extract to different pathogenic bacteria

[0017] Using the method of measuring the inhibition zone of the drug-containing PDA medium (25°C, dark culture conditions), the six plant pathogenic fungi Fusarium graminearum, Rhizoctonia solani, Magnaporthe oryzae, Glososporum anthracnose, and Botrytis cinerea and tomato early blight inhibition test. The test results are as follows.

[0018]

Embodiment 3

[0019] Embodiment 3: the indoor inhibitory action of monomer compound of the present invention to Botrytis cinerea

[0020] Tested species: Botrytis cinerea

[0021] Experimental method: the test bacteria were activated on PDA (potato dextrose agar medium) for 72 hours. The polyphenol compound obtained by separation and extraction was prepared into a dilution solution with a concentration of 62.5 µg / ml to 2000 µg / ml by 2-fold concentration dilution method, and then mixed evenly (v / v) with the drug solution and PDA medium at a ratio of 1:9 to obtain a concentration of 6.25 µg / ml~200µg / ml drug-containing medium, each treatment was poured into 4 flat plates (diameter 9cm), after cooling, 6mm bacteria cake was placed in the center of the plate, cultured at 25±1°C, and the blank control bacteria When the cake grew to the edge of the plate, the diameter of the colony of each treatment was measured by the cross method of vernier calipers, and the bacteriostatic rate of each treatmen...

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PUM

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Abstract

The invention relates to application of an apple residue and apple leaf extract to prevention and control of phytopathogen. The apple residue and apple leaf extract contains polyphenols of phlorizin,quercitrin, isoquercitrin, quercetin-3-D-xyloside and quercetin-3-D-glucosidase arabinofuranoside, and can be applied to prevention and control of diseases caused by fungi and diseases caused by bacteria for various grain crops, economic crops, vegetables, fruit trees, melons and the like. A biological sterilizing preparation prepared by extracting a mixture or a single substance is environmentally friendly, is safe to human beings and livestock, and can be used for effectively preventing and controlling various plant diseases.

Description

technical field [0001] The invention relates to a method for extracting bactericidal activity of apple pomace and apple leaf extract and its application in plant disease prevention and control, belonging to the field of plant agricultural protection. Background technique [0002] Apples are the fruit of plants of the genus Malus in the Rosaceae family. Apple is currently the largest fruit in my country, with the highest cultivation area and output. It is a rare agricultural product in my country that occupies a competitive advantage in the international market. Apples are mainly consumed fresh, followed by primary processing, such as concentrated apple juice, cider, and jam. In these processing processes, a large amount of by-products of apple pomace will be produced, about several million tons per year, but the probability of its effective use is very low, and a small amount will be used to extract pectin and as feed, fertilizer, fuel, Most of the others are directly trea...

Claims

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Application Information

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IPC IPC(8): A01N35/04A01N43/16A01P3/00
CPCA01N35/04A01N43/16
Inventor 魏芸倪汉文路艳珍
Owner BEIJING UNIV OF CHEM TECH
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