Cordyceps militaris peanut and preparing method thereof
A technology of Cordyceps militaris and peanuts, which can be applied to botany equipment and methods, fertilizer mixtures, gardening, etc., and can solve problems that have not been reported before
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Embodiment 1
[0022] 1) Peanut soaking
[0023] Weigh 250 g of peanuts of Huayu No. 23 variety, add 500 g of water, and soak them at room temperature for 12 hours, so that the moisture content of the soaked peanuts is controlled within the range of 100-120%.
[0024] 2) Sterilization
[0025] After the soaked peanuts were drained, they were divided into conical flasks, covered with stoppers, sterilized at a temperature of 121°C for 30 minutes, and then inoculated after cooling.
[0026] 3) Slant culture of Cordyceps militaris strains
[0027] The preserved Cordyceps militaris strain is inoculated on the slant medium, and cultivated for 7 to 9 days at a temperature of 23 ° C. The composition of the slant medium is: potato 18-22%, glucose 1-3%, peptone 0.1% ~1.0%, potassium dihydrogen phosphate 0.1~0.5%, magnesium sulfate 0.1~0.3%, vitamin B 1 0.008~ 0.02%, agar 1-3%. A specific formula is as follows: potato 20%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.3%, magnesium...
Embodiment 2
[0047] 1) Peanut soaking
[0048] Weigh 250 g of peanuts of Huayu No. 23 variety, add water at a ratio of 1:2 to 1:5, and soak them at room temperature for 10 to 16 hours to control the water content of peanuts at 100 to 120%.
[0049] 2) Peanut cellulase treatment
[0050] Drain the soaked peanuts, put them into a conical flask, add citric acid-disodium hydrogen phosphate buffer at a ratio of 1:3 to 5, and carry out cellulase treatment at 50°C for 1 to 3 hours. The amount of enzyme is 8000-12000U / g peanuts, rinsed with running water for 10-20 minutes, drained, and dried in a dry place for 5-8 hours. Cellulase is derived from green wood enzyme, and its activity is 11000U / mg.
[0051] 3) Sterilization
[0052] Peeled peanut granules of mesh 10 to 15 were divided into triangular flasks, covered with stoppers, sterilized at 121°C for 30 minutes, and then inoculated after cooling.
[0053] 4) Slant culture of Cordyceps militaris strains
[0054] The preserved Cordyceps milita...
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