Micro ventilation lime wood antrodia camphorate cultivation method
A kind of cultivation method and technique of basswood, applied in the field of micro-breathable basswood cultivation of Antrodia camphorata
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[0046] 1 Materials and methods
[0047] 1.1 Materials
[0048] 1.1.1 Tested strains: Antrodia Camphorata: It is collected by the Provincial Key Laboratory of Applied Fungi of Qingdao Agricultural University and can be released to the public. This experiment is not limited to this strain, but also applicable to other strains.
[0049] 1.1.2 Mother medium: glucose 2%, banana powder 0.5%, yeast extract 0.5%, peptone 0.5%, MgSO4 0.3%, KH2PO4 0.3%, citric acid 0.05%, (NH4)2SO4 0.05%, agar 2%, Camphor wood extract, pH4.5
[0050] 1.1.3 Liquid fermentation medium: glucose 2%, banana powder 0.5%, yeast extract 0.5%, peptone 0.5%, MgSO4 0.3%, KH2PO4 0.3%, citric acid 0.05%, (NH4)2SO4 0.05%, camphor wood extract 1. Preparation of pH4.5 camphor wood extract: Take 500g of camphor wood debris, add 5000mL of water, cook until concentrated to 1 / 10 of the original volume, the resulting liquid is camphor wood extract, and the dosage is 100mL of liquid fermentation medium Add 50mL camphor w...
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