Method for extracting, separating and purifying Astragaloside IV from Astragalus mongholicus

A technology for astragaloside IV and astragalus, which is applied in the field of extracting, separating and purifying astragaloside IV from astragalus, can solve the problems of low content, difficulty in separation and purification, etc., and achieve the effects of high purity, high yield, and simple and easy method.

Inactive Publication Date: 2010-07-14
NORTHEAST FORESTRY UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the problem that the content of astragaloside IV in plants is very low (about 0.04%) and the separation and purification are quite difficult, the purpose of the present invention is to provide a kind of traditional Chinese medicine Radix Astragali as raw material, through the original homogenate extraction-mixed enzyme induction Biotransformation technology, negative pressure cavitation mixing extraction technology, hydrolysis conversion technology of saponin derivatives, liquid-liquid extraction technology, macroporous adsorption resin enrichment technology, normal phase silica gel medium pressure column chromatography technology and low temperature crystallization and recrystallization technology A series of high-efficiency extraction, separation and purification technical means with independent intellectual property rights, so as to obtain a high-yield, high-purity astragaloside IV method simply and quickly

Method used

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  • Method for extracting, separating and purifying Astragaloside IV from Astragalus mongholicus
  • Method for extracting, separating and purifying Astragaloside IV from Astragalus mongholicus

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Embodiment 1

[0032] Accurately weigh 1000 g of traditional Chinese medicine Astragalus membranaceus, dissolve 0.2% solid weight of mixed enzymes (cellulase, pectinase, β-glucosidase) in 10 times the volume of warm water, and continuously homogenize and extract 3 times, each time for 1 min. Put the homogenate in a constant temperature shaker at 30°C for constant temperature enzymolysis for 12 hours, after suction filtration of the enzyme-inducing solution, add 70% ethanol to the filter residue for negative pressure cavitation extraction, the pressure is 0.05Mpa, and the solid-liquid ratio is 1:10 (g:mL ), extracted at room temperature 3 times, each time for 45min, the enzymatic hydrolyzate and the extract after suction filtration were combined, concentrated under reduced pressure to dryness, then dissolved in water, adjusted to pH 8 with ammonia water, hydrolyzed for 12h, and the hydrolyzed solution obtained was An equal volume of n-butanol was used as a solvent, extracted three times, and c...

Embodiment 2

[0034] Accurately weigh 1000 g of the traditional Chinese medicine Astragalus membranaceus, dissolve 0.5% solid weight of mixed enzymes (cellulase, pectinase, β-glucosidase) in 15 times the volume of warm water, and continuously homogenize and extract 3 times, each time for 2 minutes. Put the homogenate in a constant temperature shaker at 30°C for constant temperature enzymolysis for 24 hours. After the enzyme-inducing solution is suction-filtered, the filter residue is extracted by negative pressure cavitation with 80% ethanol. The pressure is 0.06Mpa, and the solid-liquid ratio is 1:15 (g:mL ), extraction at room temperature 3 times, 60min each time, the enzymolysis solution after suction filtration and the extraction solution were combined, concentrated under reduced pressure to dryness and then dissolved in water, adjusted to pH 9 with ammonia water, hydrolyzed for 24h, and the hydrolyzed solution obtained was An equal volume of n-butanol was used as a solvent, extracted 4 ...

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Abstract

The invention relates to a method for extracting, separating and purifying a functionality active component Astragaloside IV from a common used traditional Chinese medicine Astragalus mongholicus, and aims to provide a simple, safe, economic and effective method for extracting, separating and purifying the Astragaloside IV from the Astragalus mongholicus with high purity. The adopted technical scheme is as follows: taking traditional Chinese medicine Astragalus mongholicus as a raw material; and adopting a series of original and efficient technologies for extracting, separation and purifying such as homogenate extraction-mixing enzyme induction biotransformation technology, negative pressure cavitation suspension extraction technology, saponin derivant hydrolysis converting technology, liquid-liquid extraction technology, macroporous absorption resin enrichment technology, normal phase silica gel medium pressure column chromatography technology and devitrification at a low temperature, recrystallization technology and the like to obtain the Astragaloside IV with high purity and yield, wherein the yield thereof can be more than 0.08% and the purity can be more than 95%. The raw material used in the invention is the common used traditional Chinese medicine Astragalus mongholicus, and has abundant resources; the production process of the Astragaloside IV is simple and practicable; the target compound has high yield and purity; and the method is suitable for the industry production and application, has high additional value and excellent market application prospective.

Description

technical field [0001] The invention relates to a method for extracting, isolating and purifying functional active ingredients from plants, in particular to a method for extracting, isolating and purifying astragaloside IV from commonly used Chinese medicinal material Astragalus membranaceus. Background technique [0002] Astragalus is known as "the best of all qi-tonifying medicines". It is a precious Chinese herbal medicine. It was first recorded in the first herbal book "Shen Nong's Materia Medica" in ancient my country. The dry root of Bunge var. mongholicus (Bunge) Hsiao or Astragalus membranaceus (Fisch.) Bunge. Mongolian Astragalus membranaceus is distributed in some provinces in Northeast China and North China; Astragalus membranaceus is distributed in North China, Northwest China, Northeast China and some provinces in Southwest China. Astragalus is mainly distributed in temperate and warm temperate regions in my country, and is a deep-rooted plant. In the northern ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J53/00
Inventor 付玉杰祖元刚闫明明刘威罗猛李庆勇陈彩云
Owner NORTHEAST FORESTRY UNIVERSITY
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