Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle

A molecular marker and muscle technology, which can be used in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. It can solve the problems of pH difference and affecting the speed of glycolysis.

Inactive Publication Date: 2015-04-01
HUAZHONG AGRI UNIV
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Problems solved by technology

Therefore, the activity of lactate dehydrogenase will directly affect the rate of glycolysis, resulting in the difference in pH

Method used

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  • Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle
  • Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle
  • Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle

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Embodiment 1

[0022] (1) Pig Genomic DNA Extraction

[0023] The pig breed for extracting the pig genome DNA in the present invention is Large White pig (Hubei Jinlin Original Breeding Animal Husbandry Co., Ltd.). Pig population DNA samples were all extracted using the Genomic DNA Kit (TIANamp Genomic DNA Kit) produced by Tiangen Biochemical (Beijing) Technology Co., Ltd. The specific operation steps are as follows:

[0024] 1. Put the ear sample of Large White pig into a 2mL EP tube, wipe clean ophthalmic surgical scissors with alcohol cotton and cut into a paste, then add 200 μL buffer GA (included with the kit).

[0025] 2. Add 20 μL proteinase K solution (included with the kit), mix well and place in a 56°C water bath for overnight digestion.

[0026] 3. Add 200 μL buffer GB, mix thoroughly by inversion, place at 70°C for 10 minutes, the solution should become clear, and centrifuge briefly to remove water droplets on the inner wall of the tube.

[0027] 4. Add 200 μL of absolute ethan...

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Abstract

The invention belongs to the technical field of preparation of livestock molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to a pH (Potential of Hydrogen) value character of pig muscle. The molecular marker is obtained by LDHA (Lactate DehydrogenaseHydroxyacyl) gene cloning and has a nucleotide sequence as shown in Figure 4.In the sequence as shown in Figure 4, the 495th position has allele G or A base substitution, and the 635th position of the sequence has allele C or T base substitution. The new molecular marker is provided for assistant selection of pig markers.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to the application of a molecular marker related to the pH value traits of pork. Background technique [0002] The main source of meat products in my country is pork. With the development of pig breeding industry, its output has been able to meet people's needs, so the improvement of pork quality has become a hot issue in the study of pig breeding. The improvement of meat quality can be considered from many aspects such as genetics, nutrition and disease prevention and treatment, among which genetic factors are the internal reasons. Breeding by means of molecular genetics can effectively improve the quality of pork from the source. [0003] Meat quality evaluation indicators include meat color, pH value, water resistance, drip loss, marbling, etc. , and the pH value is closely related to the meat color and water strength as well as the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 赵书红黄济贺佳玮曹建华李世军李长春李新云
Owner HUAZHONG AGRI UNIV
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