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RT-LAMP detection kit and detection method of infectious myonecrosis viruses

An RT-LAMP and detection kit technology, applied in the biological field, can solve problems such as inconvenient promotion, achieve the effects of rapid and accurate identification, broad promotion and application prospects, and low requirements for experimental instruments

Inactive Publication Date: 2016-11-09
山东拜尔检测股份有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the above detection methods are only suitable for operation in the laboratory, and are not convenient for large-scale promotion.

Method used

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  • RT-LAMP detection kit and detection method of infectious myonecrosis viruses
  • RT-LAMP detection kit and detection method of infectious myonecrosis viruses
  • RT-LAMP detection kit and detection method of infectious myonecrosis viruses

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Embodiment Construction

[0049] The present invention will be described in detail through specific examples below. These embodiments are provided for a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0050] "Includes" or "comprises" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is a preferred implementation mode for implementing the present invention, but the description is for the purpose of the general principles of the specification, and is not intended to limit the scope of the present invention. The scope of protection of the present invention should be defined by the appended claims.

[0051] The various reagents and instruments used in the present invention are purchased from the market unless otherwise specified.

[0052] 1. RT-LAMP primer design

[0053] First carry out ...

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Abstract

The invention discloses an RT-LAMP detection kit and a detection method of infectious myonecrosis viruses, wherein the kit consists of a mixed solution of outer primers F3 and B3 and inner primers FIP and BIP; and nucleotide sequences of the primers F3, B3, FIP and BIP are separately shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. According to the detection method, an RT-LAMP detection system is used for detecting whether a sample contains the infectious myonecrosis viruses or not. The method is simple and convenient to operate and is relatively low in requirement on experimental instruments, and the method can be completed in a normal constant-temperature water bath kettle within 60min; moreover, by virtue of a method of adding a dye to a final product and by directly observing a result with naked eyes, the method can rapidly and accurately identify the infectious myonecrosis viruses; and the method has a broad popularization and application prospect in prawn aquaculture.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an RT-LAMP detection kit and a detection method for infectious myonecrosis virus. Background technique [0002] Shrimp infectious myonecrosis (infectious myonecrosis, IMN) first broke out in August 2002 in the Piaui state of Brazil's Litopenaeus vannamei farm, and soon spread in the northeastern coast of Brazil, and has now spread to Asia. Muscle necrosis occurs in the distal body segment and tail fan of the shrimp infected by the disease, and the muscle is whitish and opaque, and the appearance is scorched. Histological observation shows that the disease mainly infects the muscle tissue and lymphoid organs of prawns, and is often accompanied by edema and blood cell fibrosis. There is often fluid accumulation between necrotic muscle fibers and fibrotic blood cells. Dark basophilic inclusion bodies. Because it can be infected at all stages in the breeding process, the cumulative mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/6844C12Q1/701C12Q2531/119C12Q2563/107
Inventor 刘清亮代玲玲孙艳丽张瑗孙琳琳
Owner 山东拜尔检测股份有限公司
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