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Application of adiponcetin gene expression level in beef cattle muscle marbling grade identification

A technology of gene expression level and adiponectin, applied in the field of level identification, can solve the problem of low sensitivity and achieve the effect of accurate and reliable comparison value, good sensitivity and repeatability

Inactive Publication Date: 2012-05-30
JILIN AGRI SCI & TECH COLLEGE
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AI Technical Summary

Problems solved by technology

Using competitive quantitative PCR, the main problem is that it needs to construct a competitive template, which is less sensitive than real-time fluorescence quantitative technology

Method used

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  • Application of adiponcetin gene expression level in beef cattle muscle marbling grade identification
  • Application of adiponcetin gene expression level in beef cattle muscle marbling grade identification
  • Application of adiponcetin gene expression level in beef cattle muscle marbling grade identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This experiment mainly observed the ADPN mRNA level in the fat tissue of Yanbian Yellow Cattle and Grassland Red Cattle at different fattening stages and the content of intramuscular fat in the main muscle mass, and further observed the relationship with the formation of muscle marbling in beef cattle. The relationship between the formation of beef cattle muscle marbling provides a theoretical basis for exploring genes related to intramuscular fat deposition in beef cattle.

[0057] 1. Materials and Methods

[0058] 1.1 Selection and handling of test cattle

[0059] 54 Yanbian Yellow Cattle and 54 Grassland Red Cattle with moderate body condition, healthy and disease-free were purchased and randomly divided into three parallel groups, with 18 cattle in each repetition group, and each cattle was fed in a single circle and in a trough. The average body weight was about 300kg, and the average age was 24 months. There was no significant difference in body weight between g...

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Abstract

The invention relates to an application of an adiponcetin gene expression level in beef cattle muscle marbling grade identification, which is characterized in that a cloned adiponcetin gene cDNA complete sequence is used to establish a real-time fluorescence quantitative detection method of adiponcetin mRNA; and the application of the adiponcetin gene expression level in beef cattle muscle marbling grade identification is determined. The method which adopts fluorescence quantitative RT-PCT to detect ADPNmRNA has very good sensitivity and repeatability. In the detection results, a copy number is used to express the content of an initial template, which is more accurate and reliable than a comparison value in semi-quantitative RT-PCR; the ADPNmRNA level is negatively correlated with the muscle marbling of fattening cattle (Yanbian cattle r=-0.87; P<0.001, n=9) and (red steppe cattle r=-0.79; P<0.001, n=9). The accuracy rate is up to 95%.

Description

technical field [0001] The invention relates to the application of adiponectin gene expression level in beef cattle muscle marbling grade identification, which is used for monitoring the grade of beef cattle and determining the most suitable slaughtering time. Background technique [0002] The research on the molecular level of livestock and poultry meat quality in my country’s animal husbandry industry is still in its infancy. The growth and development, metabolism, genetic variation, immunity and disease of livestock and poultry are essentially the result of changes in the expression and regulation of animal genes. The thorough elucidation of the phenomenon ultimately needs to be explained at the gene level, so it is necessary to explain various genes and animal functions, the mechanism of action on the body, and other issues at the molecular level. With the continuous development of molecular biology techniques, more and more animal genes related to metabolism have been cl...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 张辉常维毅丛立新李鹏陈晓霞
Owner JILIN AGRI SCI & TECH COLLEGE
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