Method for producing Anrodia camphorata mycelia based on solid-state surface culture
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A solid surface, culture method technology, applied in the field of bioengineering, can solve problems such as high energy consumption, achieve the effect of maintaining content and shortening production cycle
Inactive Publication Date: 2010-09-08
JIANGNAN UNIV
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Freeze-drying works well, but consumes a lot of energy and requires expensive freeze-drying equipment
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Embodiment 1
[0044] Example 1: (Based on solid surface culture method in culture flask)
[0045] Strain: Anrodia camphorata.
[0046] Seed medium (g / L): glucose 20, Na 2 HPO 4 0.5, MgSO 4 0.5, citric acid 0.5; corn steep liquor 40mL / L, soybean hydrolysate 20mL / L.
[0047] Seed culture conditions: culture temperature 28°C, shaker speed 120r / m, pH natural, culture time 4d.
[0048] Solid medium (g / L): Use food-grade grains (rice, millet, glutinous rice, etc.) to ferment. The filling amount is 60~180g / L, based on the dry weight of the added materials, the ratio of rice to nutrient solution is 6:1 to 1:1, nutrient solution (g / L): glucose 10-30, Na 2 HPO 4 0.1~5, MgSO 4 0.1~5, soybean hydrolysate 10~100mL / L, corn steep liquor 10~80mL / L, pH 1.0~7.0. Sterilize at 121℃ for 20min.
[0049] Solid-state culture conditions: inoculation amount: 0.05-4mL / g, placed in a culture room with a temperature of 20-35°C and a humidity of 60%-90%, and the fermentation time is 15-120 days;
[0050] Manual spin bottle me...
Embodiment 2
[0053] Example 2 (Based on the method of culturing the reactor on the surface and adding solid culture medium):
[0054] Strain: Anrodia camphorata.
[0055] Seed medium (g / L): glucose 10-30, Na 2 HPO 4 0.5, MgSO 4 0.5, citric acid 0.5; corn steep liquor 40mL / L, soybean hydrolyzate 20mL / L.
[0056] Seed culture conditions: culture temperature is 28°C, shaker speed is 120r / m, pH is natural, culture time is 4d.
[0057] Solid medium (g / L): Mix different grains (rice, millet, glutinous rice, etc.) according to the most and least weight ratio of 1:4 to 4:1. Based on the dry weight of the added materials, the ratio of rice to nutrient solution is 6:1~1:1, nutrient solution (g / L): glucose 10~30, Na 2 HPO 4 0.1~5, MgSO 4 0.1~5, soybean hydrolysate 10~100mL / L, corn steep liquor 10~80mL / L, pH 1.0~7.0.
[0058] Solid-state culture conditions: the 0.05-4mL / g seed solution and 0.05-4mL / g nutrient solution are connected to the sterilized and cooled culture medium, and placed in a culture room w...
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Abstract
The invention discloses a method for producing Anrodia camphorata mycelia based on solid-state surface culture, and belongs to the field of bioengineering. The method comprises the following steps of: screening components of a culture medium of the Anrodia camphorate and optimizing the proportion of the cereal raw material in the culture medium so that the culture medium is firmly adsorbed to the inner wall of a culture vessel or the culture surface to bring convenience to the surface culture of the Anrodia camphorata mycelia; inoculating the Anrodia camphorata mycelia in the culture surface of a reactor and culturing the Anrodia camphorata mycelia in the environment of certain temperature and moisture; feeding the culture medium every a certain time in batch during the surface culture so as to timely replenish the nutrient components required by the growth of the Anrodia camphorata mycelia and making the growth environment of the Anrodia camphorata mycelia close to the natural culture environment until a large amount of mycelia or large colony is formed; and quickly drying the Anrodia camphorata culture at a low temperature after the culture. The method of the invention makes the growth environment of the Anrodia camphorata mycelia close to that of the wild Anrodia camphorate and improves the productivity of the Anrodia camphorata culture by timely feeding the culture medium in batch, so that the obtained Anrodia camphorata culture has high content of active ingredient and closer efficacy to the wild fruit body.
Description
Technical field [0001] The invention provides a rapid surface culturing method for Antrodia camphorata mycelium, which belongs to the field of bioengineering. Background technique [0002] Anrodia camphorata, also known as Antrodia camphorata, Antrodia camphorata, Antrodia camphorata or Antrodia camphorata, Antrodia camphorata. Antrodia camphorata has magical effects such as detoxification, blood-clearing oil, detoxification and liver protection, nourishing the kidney and strengthening the heart, invigorating the stomach and intestines, analgesic and antibacterial, strengthening immunity, de-allergic, anti-cancer, and eliminating tumors. [0003] Antrodia is a fungus that grows on Cinnamomum camphora trees unique to Taiwan. It belongs to the order Apophyllum and Polyporaceae. Antrodia camphorata grows very slowly, and its growth period is limited to June to October each year. It takes a long time to form and produce fruit bodies, so natural production is scarce. The fruiting bodi...
Claims
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